Effect of insertional mutations in the pueA and pueB genes encoding two polyurethanases in Pseudomonas chlororaphis contained within a gene cluster

Aims:  To better understand the role of PueA and PueB from Pseudomonas chlororaphis in polyurethane degradation, the present study was conducted to create insertional mutants in their respective genes.

[1]  B. Crother,et al.  Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis , 2001 .

[2]  G. T. Howard,et al.  The polyester polyurethanase gene (pueA) from Pseudomonas chlororaphis encodes a lipase. , 2000, FEMS microbiology letters.

[3]  K. Jaeger,et al.  Bacterial lipolytic enzymes: classification and properties. , 1999, The Biochemical journal.

[4]  K. Omori,et al.  The ABC-exporter genes involved in the lipase secretion are clustered with the genes for lipase, alkaline protease, and serine protease homologues in Pseudomonas fluorescens no. 33. , 1999, Biochimica et biophysica acta.

[5]  J. Rhee,et al.  Identification of the tliDEF ABC Transporter Specific for Lipase in Pseudomonas fluorescensSIK W1 , 1999, Journal of bacteriology.

[6]  G. T. Howard,et al.  Growth of Pseudomonas chlororaphis on apolyester–polyurethane and the purification andcharacterization of a polyurethanase–esterase enzyme , 1999 .

[7]  K. Omori,et al.  Serratia marcescens S‐layer protein is secreted extracellularly via an ATP‐binding cassette exporter, the Lip system , 1998, Molecular microbiology.

[8]  D. Roop,et al.  Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. , 1995, Gene.

[9]  K. Omori,et al.  The three genes lipB, lipC, and lipD involved in the extracellular secretion of the Serratia marcescens lipase which lacks an N-terminal signal peptide , 1995, Journal of bacteriology.

[10]  C. Soscia,et al.  The Pseudomonas fluorescens lipase has a C‐terminal secretion signal and is secreted by a three‐component bacterial ABC‐exporter system , 1994, Molecular microbiology.

[11]  James R. Campbell,et al.  Biodegradation of a colloidal ester-based polyurethane by soil fungi , 1994 .

[12]  C Colson,et al.  Bacterial lipases. , 1994, FEMS microbiology reviews.

[13]  A. Pugsley.  The complete general secretory pathway in gram-negative bacteria. , 1993, Microbiological reviews.

[14]  Marc Reisch,et al.  Marine paint makers strive to meet environmental concerns , 1990 .

[15]  F. Winkler,et al.  Structure of human pancreatic lipase , 1990, Nature.

[16]  B. Persson,et al.  Structural features of lipoprotein lipase , 1989 .

[17]  B. Persson,et al.  Structural features of lipoprotein lipase. Lipase family relationships, binding interactions, non-equivalence of lipase cofactors, vitellogenin similarities and functional subdivision of lipoprotein lipase. , 1989, European journal of biochemistry.

[18]  D. Wales,et al.  Mechanistic Aspects of Polyurethane Biodeterioration , 1988 .

[19]  D. Jordan,et al.  Species variation in the specificity of ribulose biphosphate carboxylase/oxygenase , 1981, Nature.