Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems

The application of hydrogels as a matrix for 3-dimensional cell cultures has become an indispensable tool in tissue engineering, biotechnology and biomedical research due to the improved functionality and viability of the in vitro biological system. The combination of 3-dimensional hydrogel cell cultures with microchip technology further allows (i) spatial and temporal control of cell growth, (ii) application of defined mechanical (e.g., shear, strain, stretch) and chemical (e.g., gradients) stimuli, as well as (iii) monitoring of dynamic cellular responses using integrated sensing strategies. The main advantage of hydrogels for microfluidic cell cultures, however, is their mimicry of extracellular matrix structures including adequate porosity for cellular organization, biocompatibility, and representative stiffness, all key parameters that promote native-like tissue function. This review focuses primarily on recent advances in biologically inspired microfluidic systems that are based on 3-dimensional hydrogel cell cultures and discusses advantages and current challenges, various applications of microfluidic hydrogel cell cultures and future perspectives. JM Rosser1, I Olmos Calvo2,3, M Schlager2, M Purtscher4, F Jenner1 and P Ertl2* 1University of Veterinary Medicine, Clinic for Equine Surgery, Vienna, Austria 2AIT Austrian Institute of Technology GmbH, Biosensor Technologies, Vienna, Austria 3Department of Medicine III, Division of Rheumatology, Medical University of Vienna, Austria 4Department of Biochemical Engineering, University of Applied Sciences Technikum Wien, Vienna, Austria JM Rosser and I Olmos Calvo equally contributed to this manuscript as joint first authors Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems Citation: Rosser JM, Olmos-Calvo I, Schlager M, Purtscher M, Jenner F, et al. (2015) Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems. J Cell Biol Cell Metab 2: 005.

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