Calcium stoichiometry determination for calcium binding proteins by electrospray ionization mass spectrometry.

Electrospray ionization mass spectrometry was used for the determination of calcium-binding stoichiometry for calcium-binding proteins. Bovine calmodulin, bovine alpha-lactalbumin, and rabbit parvalbumin were found to bind specifically to 4, 1, and 2 Ca(2+) ions, respectively, in agreement with previously reported results obtained by using other physical methods. This mass spectrometry method could also be applied to proteins that bind more than one type of metal ion. The Zn(2+)- and Ca(2+)-binding stoichiometries for human stromelysin catalytic domain were determined to be 3 and 2, respectively.

[1]  H. V. Van Wart,et al.  Zinc content of promatrilysin, matrilysin and the stromelysin catalytic domain. , 1994, Biochemical and biophysical research communications.

[2]  Ivano Bertini,et al.  Nuclear magnetic resonance of paramagnetic metalloproteins , 1993 .

[3]  P. Andrews,et al.  Primary to quaternary protein structure determination with electrospray ionization and magnetic sector mass spectrometry , 1993 .

[4]  Bert L. Vallee,et al.  Zinc: biological functions and coordination motifs , 1993 .

[5]  Richard D. Smith,et al.  The observation of non‐covalent interactions in solution by electrospray ionization mass spectrometry: Promise, pitfalls and prognosis , 1993 .

[6]  Francis M. Wampler,et al.  Fourier-transform electrospray instrumentation for tandem high-resolution mass spectrometry of large molecules , 1993, Journal of the American Society for Mass Spectrometry.

[7]  C. Fenselau,et al.  Assessment of metals in reconstituted metallothioneins by electrospray mass spectrometry. , 1993, Analytical chemistry.

[8]  J. Loo,et al.  Observation of intact (heme‐bound) myoglobin by electrospray ionization on a double‐focusing mass spectrometer , 1993 .

[9]  D. Hupe,et al.  Purification and characterization of the human stromelysin catalytic domain expressed in Escherichia coli. , 1992, Biochemistry.

[10]  C. Fenselau,et al.  Electrospray analysis of proteins: A comparison of positive-ion and negative-ion mass spectra at high and low pH , 1992 .

[11]  T. Yip,et al.  Peptide-metal ion interactions in solution: Detection by laser desorption time-of-flight mass spectrometry and electrospray ionization mass spectrometry† , 1992 .

[12]  T. Yip,et al.  Recognition of transition metal ions by peptides Identification of specific metal‐binding peptides in proteolytic digest maps by UV laser desorption time‐of‐flight mass spectrometry , 1992, FEBS letters.

[13]  C. G. Edmonds,et al.  Multiply charged negative ions by electrospray ionization of polypeptides and proteins. , 1992, Analytical chemistry.

[14]  P. Kebarle,et al.  Electrospray mass spectrometry of methanol and water solutions suppression of electric discharge with SF6 gas , 1991, Journal of the American Society for Mass Spectrometry.

[15]  Richard D. Smith,et al.  Principles and practice of electrospray ionization—mass spectrometry for large polypeptides and proteins , 1991 .

[16]  B. Ganem,et al.  Observation of noncovalent enzyme-substrate and enzyme-product complexes by ion-spray mass spectrometry , 1991 .

[17]  M. Mann,et al.  Of protons or proteins , 1988 .