SensApp: a FET-open project for developing a supersensor able to detect Alzheimer's disease biomarkers in blood

The goal of the SensApp FET-Open project is to develop an innovative super-sensor that will be able to detect Alzheimer’s disease (AD) biomarkers (β-amyloid, Tau and pTAU) in peripheral blood. Considering that nowadays an accurate diagnosis of AD requires the highly invasive withdrawal and analysis of cerebrospinal fluid, SensApp will represent a breakthrough in the field of AD diagnosis thanks to the ability to detect the early stage of the disease by a simple blood collection. We call Droplet-Split-and-Stack (DSS) the new technology that will emerge from SensApp. The achievement of SensApp goal is enabled by the interdisciplinary cooperation between different research institutions and one company involved in the key fields of the project, Vrije Universiteit Brussels, VTT Technical Research Centre of Finland, University of Linz, Ginolis Ltd, IRCCS Centre “Bonino Pulejo”, under the coordination of CNR-Institute of Applied Sciences and Intelligent Systems. This communication will illustrate the progress of the activities.

[1]  Pietro Ferraro,et al.  Simple and Rapid Bioink Jet Printing for Multiscale Cell Adhesion Islands. , 2017, Macromolecular bioscience.

[2]  R. Rega,et al.  A pyroelectric-based system for sensing low abundant lactose molecules , 2019, Optical Metrology.

[3]  P. Maddalena,et al.  Direct Evidence of Polar Ordering and Investigation on Cytophilic Properties of Pyroelectrified Polymer Films by Optical Second Harmonic Generation Analysis , 2017 .

[4]  Romina Rega,et al.  Spiral formation at microscale by μ-pyro-electrospinning , 2016 .

[5]  C. Rowe,et al.  High performance plasma amyloid-β biomarkers for Alzheimer’s disease , 2018, Nature.

[6]  Brian A. Gordon,et al.  Influence of tau PET, amyloid PET, and hippocampal volume on cognition in Alzheimer disease , 2018, Neurology.

[7]  P. Ferraro,et al.  Pyro-electrification of polymer membranes for cell patterning , 2016 .

[8]  Martina Mugnano,et al.  Highly sensitive detection of low abundant molecules by pyro-electrohydro-dynamic jetting , 2020, Photonics Europe.

[9]  A. Irace,et al.  Investigation of pyroelectric fields generated by lithium niobate crystals through integrated microheaters , 2017 .

[10]  Martina Mugnano,et al.  Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation , 2020, Sensors.

[11]  K. Marcus,et al.  Amyloid-β as a biomarker for Alzheimer’s disease: quantification methods in body fluids , 2015, Expert review of proteomics.

[12]  Pietro Ferraro,et al.  Bipolar Patterning of Polymer Membranes by Pyroelectrification. , 2016, Advanced materials.

[13]  P. Ferraro,et al.  A skin-over-liquid platform with compliant microbumps actuated by pyro-EHD pressure , 2019, NPG Asia Materials.

[14]  P. Ferraro,et al.  Maskless Arrayed Nanofiber Mats by Bipolar Pyroelectrospinning. , 2019, ACS applied materials & interfaces.

[15]  M. Vidali,et al.  Diagnostic accuracy of cerebrospinal fluid biomarkers measured by chemiluminescent enzyme immunoassay for Alzheimer disease diagnosis , 2020, Scandinavian journal of clinical and laboratory investigation.

[16]  D. Selkoe.  Alzheimer's disease. , 2011, Cold Spring Harbor perspectives in biology.

[17]  L. Lannfelt,et al.  Quantification of Alzheimer Amyloid Peptides Ending at Residues 40 and 42 by Novel ELISA Systems , 1999 .

[18]  Yanchun Han,et al.  Inkjet printing of viscoelastic polymer inks , 2017 .

[19]  A. Fagan,et al.  Clinical utility of cerebrospinal fluid biomarkers in the diagnosis of early Alzheimer's disease , 2015, Alzheimer's & Dementia.

[20]  P. Ferraro,et al.  Easy Printing of High Viscous Microdots by Spontaneous Breakup of Thin Fibers. , 2018, ACS applied materials & interfaces.

[21]  A. Abbott,et al.  Mechanics of nozzle clogging during direct ink writing of fiber-reinforced composites , 2020 .

[22]  Anders Wallin,et al.  Evaluation of plasma Aβ40 and Aβ42 as predictors of conversion to Alzheimer's disease in patients with mild cognitive impairment , 2010, Neurobiology of Aging.

[23]  P. Ferraro,et al.  Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro , 2019, Front. Chem..

[24]  V Marchesano,et al.  Pyroelectric Effect Enables Simple and Rapid Evaluation of Biofilm Formation. , 2018, ACS applied materials & interfaces.

[25]  P. Ferraro,et al.  Twice electric field poling for engineering multiperiodic Hex-PPLN microstructures , 2017 .