A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization

Egr-1 is an early growth response gene that displays fos-like induction kinetics in fibroblasts, epithelial cells, and lymphocytes following mitogenic stimulation. Sequence analysis of murine Egr-1 cDNA predicts a protein with three DNA binding zinc fingers. The human EGR1 gene maps to chromosome 5 (bands 5q23-31). Egr-1 mRNA increases dramatically during cardiac and neural cell differentiation, and following membrane depolarization both in vitro and in vivo. Thus, Egr-1 and c-fos are often coregulated with strikingly similar kinetics. These results, in conjunction with the Egr-1 primary structure, suggest that Egr-1 may function as a transcriptional regulator in diverse biological processes.

[1]  J. Rowley,et al.  Evidence for the involvement of GM-CSF and FMS in the deletion (5q) in myeloid disorders. , 1986, Science.

[2]  I. Verma,et al.  Tissue and cell type-specific expression of two human c-onc genes , 1983, Nature.

[3]  A. Klug,et al.  An underlying repeat in some transcriptional control sequences corresponding to half a double helical turn of DNA , 1986, Cell.

[4]  M. McBurney,et al.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line , 1982, Nature.

[5]  L. Lau,et al.  Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells. , 1985, The EMBO journal.

[6]  M. Greenberg,et al.  Nerve growth factor and epidermal growth factor induce rapid transient changes in proto-oncogene transcription in PC12 cells. , 1985, The Journal of biological chemistry.

[7]  J. Shine,et al.  Sequence and expression of human estrogen receptor complementary DNA. , 1986, Science.

[8]  D. Melton,et al.  Xfin: an embryonic gene encoding a multifingered protein in Xenopus. , 1987, The EMBO journal.

[9]  P Argos,et al.  The primary structure of transcription factor TFIIIA has 12 consecutive repeats , 1985, FEBS letters.

[10]  M. Wickens,et al.  Role of the conserved AAUAAA sequence: four AAUAAA point mutants prevent messenger RNA 3' end formation. , 1984, Science.

[11]  E. Adamson,et al.  Induction of c-fos and AFP expression in a differentiating teratocarcinoma cell line. , 1986, Experimental cell research.

[12]  A. Lassar,et al.  Transcription of class III genes: formation of preinitiation complexes. , 1983, Science.

[13]  G. Tricot,et al.  The 5q-anomaly. , 1985, Cancer genetics and cytogenetics.

[14]  T. Curran,et al.  The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose. , 1986, Science.

[15]  J. Rowley,et al.  Evidence for two distinct c-src loci on human chromosomes 1 and 20 , 1984, Nature.

[16]  R. Evans,et al.  The c-erb-A gene encodes a thyroid hormone receptor , 1986, Nature.

[17]  V. Sukhatme,et al.  A novel early growth response gene rapidly induced by fibroblast, epithelial cell and lymphocyte mitogens. , 1987, Oncogene research.

[18]  H. Pelham,et al.  A specific transcription factor that can bind either the 5S RNA gene or 5S RNA. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[19]  L. Lau,et al.  Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. O'fallon,et al.  Clinical characteristics and prognosis of 50 patients with a myeloproliferative syndrome and deletion of part of the long arm of chromosome 5. , 1985, Blood.

[21]  P. Gruss,et al.  A multigene family encoding several “finger” structures is present and differentially active in mammalian genomes , 1987, Cell.

[22]  R. Larson,et al.  Clinical and cytogenetic correlations in 63 patients with therapy-related myelodysplastic syndromes and acute nonlymphocytic leukemia: further evidence for characteristic abnormalities of chromosomes no. 5 and 7. , 1986, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[23]  H. Blumberg,et al.  Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA , 1986, Nature.

[24]  I. Verma,et al.  Induction of the proto-oncogene fos by nerve growth factor. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[25]  Brent H. Cochran,et al.  Molecular cloning of gene sequences regulated by platelet-derived growth factor , 1983, Cell.

[26]  A. Eisen,et al.  Two zinc fingers of a yeast regulatory protein shown by genetic evidence to be essential for its function , 1987, Nature.

[27]  G. Shaw,et al.  A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation , 1986, Cell.

[28]  M. Mudryj,et al.  Transcriptional activation encoded by the v-fos gene. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[29]  G. Guroff.  PC12 Cells as a Model of Neuronal Differentiation , 1985 .

[30]  H. Jäckle,et al.  A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene , 1986, Cell.

[31]  T. Curran,et al.  Superinduction of c-fos by nerve growth factor in the presence of peripherally active benzodiazepines. , 1985, Science.

[32]  J. Milbrandt,et al.  A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor. , 1987, Science.

[33]  J. Bishop.  The molecular genetics of cancer. , 1987, Science.

[34]  A Klug,et al.  Repetitive zinc‐binding domains in the protein transcription factor IIIA from Xenopus oocytes. , 1985, The EMBO journal.

[35]  I. Verma,et al.  The fos oncogene. , 1987, Advances in cancer research.

[36]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[37]  P. Argos,et al.  Fingers and helices , 1986, Nature.

[38]  J. Berg,et al.  Potential metal-binding domains in nucleic acid binding proteins. , 1986, Science.

[39]  Robert Tjian,et al.  A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication , 1987, Cell.

[40]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[41]  M. Kozak.  An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. , 1987, Nucleic acids research.

[42]  H. Jäckle,et al.  Structural homology of the product of the Drosophila Krüppel gene with Xenopus transcription factor IIIA , 1986, Nature.

[43]  T. Curran,et al.  Mapping patterns of c-fos expression in the central nervous system after seizure. , 1987, Science.