Immunohistochemical study on the expression of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebellum of the nNOS knock-out(-/-) mice

Nitric Oxide (NO) actively participates in the regulation of neuronal intracellular Ca2+ levels by modulating the activity of various channels and receptors. To test the possibility that modulation of Ca2+ buffer protein expression level by NO participates in this regulatory effect, we examined expression of calbindin-D28k, calretinin, and parvalbumin in the cerebellum of neuronal NO synthase knock-out (nNOS(-/-)) mice using immunohistochemistry. We observed that in the cerebellar cortex of the nNOS(-/-) mice, expression of calbindin-D28k and parvalbumin were significantly increased while expression of calretinin was significantly decreased. These results suggest another mechanism by which NO can participate in the regulation of Ca2+ homeostasis.

[1]  S. Oda,et al.  Altered neuronal nitric oxide synthase expression in the cerebellum of calcium channel mutant mice , 2003, Brain Research.

[2]  S. Schiffmann,et al.  ‘New’ functions for ‘old’ proteins: The role of the calcium-binding proteins calbindin D-28k, calretinin and parvalbumin, in cerebellar physiology. Studies with knockout mice , 2002, The Cerebellum.

[3]  B. Schwaller,et al.  Calretinin and calbindin D-28k, but not parvalbumin protect against glutamate-induced delayed excitotoxicity in transfected N18–RE 105 neuroblastoma–retina hybrid cells , 2002, Brain Research.

[4]  J. Esplugues.  NO as a signalling molecule in the nervous system , 2002, British journal of pharmacology.

[5]  B. Schwaller,et al.  Calretinin and calbindin D-28k delay the onset of cell death after excitotoxic stimulation in transfected P19 cells , 2001, Brain Research.

[6]  W. C. Groat,et al.  Nitric Oxide Modulates Ca2+ Channels in Dorsal Root Ganglion Neurons Innervating Rat Urinary Bladder , 2001 .

[7]  M. Meyer,et al.  Cre recombinase expression in cerebellar Purkinje cells , 2000, Genesis.

[8]  Suk-Ho Lee,et al.  Kinetics of Ca2+ binding to parvalbumin in bovine chromaffin cells: implications for [Ca2+] transients of neuronal dendrites , 2000, The Journal of physiology.

[9]  J. Roder,et al.  Cyclic GMP-dependent feedback inhibition of AMPA receptors is independent of PKG , 2000, Nature Neuroscience.

[10]  Y. Chung,et al.  Immunohistochemical study on the distribution of neuronal voltage-gated calcium channels in the rat cerebellum , 2000, Brain Research.

[11]  B. Schwaller,et al.  Neurodegenerative and morphogenic changes in a mouse model of temporal lobe epilepsy do not depend on the expression of the calcium-binding proteins parvalbumin, calbindin, or calretinin , 2000, Neuroscience.

[12]  J. Fratkin,et al.  Relationship between ataxin-1 nuclear inclusions and Purkinje cell specific proteins in SCA-1 transgenic mice , 2000, Journal of the Neurological Sciences.

[13]  G. Cheron,et al.  Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[14]  R. Nelson,et al.  Nocturnal motor coordination deficits in neuronal nitric oxide synthase knock-out mice , 1999, Neuroscience.

[15]  O. Garaschuk,et al.  Ataxia and altered dendritic calcium signaling in mice carrying a targeted null mutation of the calbindin D28k gene. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[16]  H. Thoenen,et al.  Vulnerability of Midbrain Dopaminergic Neurons in Calbindin‐D28k‐deficient Mice: Lack of Evidence for a Neuroprotective Role of Endogenous Calbindin in MPTPtreated and Weaver Mice , 1997, The European journal of neuroscience.

[17]  G. Bertini,et al.  The chemical heterogeneity of cortical interneurons: Nitric oxide synthase vs. calbindin and parvalbumin immunoreactivity in the rat , 1996, Brain Research Bulletin.

[18]  D. Jacobowitz,et al.  The expression of calretinin in transfected PC12 cells provides no protection against Ca(2+)-overload or trophic factor deprivation. , 1996, Biochimica et biophysica acta.

[19]  T. Dawson,et al.  NITRIC OXIDE ACTIONS IN NEUROCHEMISTRY , 1996, Neurochemistry International.

[20]  E. Clementi,et al.  The Type 2 Ryanodine Receptor of Neurosecretory PC12 Cells Is Activated by Cyclic ADP-ribose , 1996, The Journal of Biological Chemistry.

[21]  S. Snyder,et al.  Behavioural abnormalities in male mice lacking neuronal nitric oxide synthase , 1995, Nature.

[22]  S. Snyder,et al.  Targeted disruption of the neuronal nitric oxide synthase gene , 1993, Cell.

[23]  R. Miller,et al.  Calcium buffering properties of calbindin D28k and parvalbumin in rat sensory neurones. , 1993, The Journal of physiology.

[24]  J. Aijón,et al.  Infrequent cellular coexistence of NADPH-diaphorase and calretinin in the neurosecretory nuclei and adjacent areas of the rat hypothalamus , 1993, Journal of Chemical Neuroanatomy.

[25]  Joseph Loscalzo,et al.  A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds , 1993, Nature.

[26]  C. Geula,et al.  Differential localization of nadph-diaphorase and calbindin-D28k within the cholinergic neurons of the basal forebrain, striatum and brainstem in the rat, monkey, baboon and human , 1993, Neuroscience.

[27]  F. Murad,et al.  Characterization and localization of nitric oxide synthase in non‐adrenergic non‐cholinergic nerves from bovine retractor penis muscles , 1992, British journal of pharmacology.

[28]  S. Lipton,et al.  Effect of nitric oxide production on the redox modulatory site of the NMDA receptor-channel complex , 1992, Neuron.

[29]  O. Manzoni,et al.  Nitric oxide-induced blockade of NMDA receptors , 1992, Neuron.

[30]  J. Rogers,et al.  Calretinin in rat brain: An immunohistochemical study , 1992, Neuroscience.

[31]  M. Celio,et al.  Calbindin D-28k and parvalbumin in the rat nervous system , 1990, Neuroscience.

[32]  Richard Graham Knowles,et al.  Formation of nitric oxide from L-arginine in the central nervous system: a transduction mechanism for stimulation of the soluble guanylate cyclase. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[33]  B. Alger,et al.  Cyclic GMP depresses hippocampal Ca2+ current through a mechanism independent of cGMP-dependent protein kinase , 1988, Neuron.

[34]  J. Farber,et al.  Calcium dependence of toxic cell death: a final common pathway. , 1979, Science.

[35]  L. Abbott,et al.  Neuronal nitric oxide synthase expression in cerebellar mutant mice , 2008, The Cerebellum.

[36]  G. Cheron,et al.  Electrophysiological behavior of Purkinje cells and motor coordination in calretinin knock-out mice. , 2000, Progress in brain research.