Chemically Specific Imaging of Cryptosporidium Oocysts Using Coherent Anti-stokes Raman Scattering (cars) Microscopy

We demonstrate the application of coherent anti‐Stokes Raman scattering microscopy for the rapid, label‐free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti‐Stokes Raman scattering microscopy, demonstrating its capability for the rapid detection of cryptosporidium at the single oocyst level. We discuss the applicability of such a technique in a near‐real time automated water testing system.

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