Genetic Identification of Hatchery Stocks of Brown Trout Using Mitochondrial DNA Polymorphism

Abstract Molecular genetics techniques provide an accurate and cost-effective way to identify hatchery strains and to measure genetic variation in fish populations. In this study, we sought to identify genetic differences at the mitochondrial DNA (mtDNA) level in two of the three hatchery strains of Michigan brown trout Salmo trutta by using restriction fragment length polymorphisms (RFLPs) of segments of mtDNA amplified by polymerase chain reaction. We show that the RFLP approach can provide valuable information about the genetic composition of Michigan brown trout strains and a method of population analysis that can be used to enhance short- and long-term fisheries management studies.

[1]  Todd C. Wills.  Comparative Abundance, Survival, and Growth of One Wild and Two Domestic Brown Trout Strains Stocked in Michigan Rivers , 2006 .

[2]  Nicolas Galtier,et al.  Population Size Does Not Influence Mitochondrial Genetic Diversity in Animals , 2006, Science.

[3]  J. Rubin,et al.  Spatial and temporal population structure of sea trout at the Island of Gotland, Sweden, delineated from mitochondrial DNA , 2002 .

[4]  L. Bernatchez.  THE EVOLUTIONARY HISTORY OF BROWN TROUT (SALMO TRUTTA L.) INFERRED FROM PHYLOGEOGRAPHIC, NESTED CLADE, AND MISMATCH ANALYSES OF MITOCHONDRIAL DNA VARIATION , 2001, Evolution; international journal of organic evolution.

[5]  E. Nielsen,et al.  Microsatellite and mitochondrial DNA polymorphism reveals life‐history dependent interbreeding between hatchery and wild brown trout (Salmo trutta L.) , 2000, Molecular ecology.

[6]  P. Janvier,et al.  The complete nucleotide sequence of the mitochondrial DNA of the dogfish, Scyliorhinus canicula. , 1998, Genetics.

[7]  E. Nielsen,et al.  Improved primer sequences for the mitochondrial ND1, ND3/4 and ND5/6 segments in salmonid fishes : application to RFLP analysis of Atlantic salmon , 1998 .

[8]  V. Loeschcke,et al.  Genetic differentiation among Danish brown trout populations, as detected RFLP analysis of PCR amplified mitochondrial DNA segments , 1996 .

[9]  R. Woodruff,et al.  Molecular Evidence for Divergent Breeding Groups of Walleye (Stizostedion vitreum) in Tributaries to Western Lake Erie , 1996 .

[10]  H. Hall,et al.  A rapid method for detecting mitochondrial DNA variation in the brown trout, Salmo trutta , 1995 .

[11]  A. Ferguson.  Genetic differences among brown trout, Salmo trutta, stocks and their importance for the conservation and management of the species , 1989 .

[12]  R. Wydoski,et al.  Tagging and marking , 1983 .