Monitoring sarcomere structure changes in whole muscle using diffuse light reflectance.

Normal biomechanical and physiological functions of striated muscles are facilitated by the repeating sarcomere units. Light scattering technique has been used in studying single extracted muscle fibers. However, few studies, if any, have been conducted to investigate the possibility of using optical detection to examine sarcomere structure changes in whole muscles. We conducted a series of experiments to demonstrate that optical scattering properties measured in whole muscle are related to changes in sarcomere structure. These results suggest that photon migration technique has a potential for characterizing in vivo tissue ultrastructure changes in whole muscle.

[1]  J. S. Chen,et al.  Optical depolarization changes in single, skinned muscle fibers. Evidence for cross-bridge involvement. , 1986, Biophysical journal.

[2]  M. Cheung,et al.  Decrease in light diffraction intensity of contracting muscle fibres , 2004, European Biophysics Journal.

[3]  N. Laing,et al.  When contractile proteins go bad: the sarcomere and skeletal muscle disease , 2005, BioEssays : news and reviews in molecular, cellular and developmental biology.

[4]  J. Fridén,et al.  Implications of muscle design on surgical reconstruction of upper extremities. , 2004, Clinical orthopaedics and related research.

[5]  R. Cassens,et al.  Further Studies on Bovine Muscle Tenderness as Influenced by Carcass Position, Sarcomere Length, and Fiber Diameter , 1965 .

[6]  Richard L Lieber,et al.  Sarcomere strain and heterogeneity correlate with injury to frog skeletal muscle fiber bundles. , 2004, Journal of applied physiology.

[7]  R L Lieber,et al.  Theory of light diffraction by single skeletal muscle fibers. , 1980, Biophysical journal.

[8]  C. Hagyard,et al.  A cold shortening effect in beef muscles , 1963 .

[9]  H. J. Swatland,et al.  A Review of Meat Spectrophotometry (300 to 800 nm) , 1989 .

[10]  L V Wang,et al.  Anisotropy in the absorption and scattering spectra of chicken breast tissue. , 1998, Applied optics.

[11]  K. Sutoh,et al.  Effect of pH on the cross-bridge arrangement in synthetic myosin filaments. , 1978, Biochemistry.

[12]  R. G. Kauffman,et al.  Muscle protein changes post mortem in relation to pork quality traits. , 1997, Meat science.

[13]  S L Jacques,et al.  In vivo determination of optical properties of normal and tumor tissue with white light reflectance and an empirical light transport model during endoscopy. , 2005, Journal of biomedical optics.

[14]  R. Sabbadini,et al.  Calcium-induced structural changes in chemically skinned muscle fibers. Detection by optical diffractometry. , 1979, Biochimica et biophysica acta.

[15]  R. Baskin,et al.  Optical depolarization changes on the diffraction pattern in the transition of skinned muscle fibers from relaxed to rigor state. , 1983, Biophysical journal.