Interspecific hybrids production between Lilium brownii var. colchesteri and its close relatives by ovary slice culture

Lilium brownii var. colchesteri (BRO) has a unique trait in its flower color. The color at anthesis is yellowish cream, and changes to white after one day. To introduce this trait and establish a sexually reproductive cultivation system for cut flower production in Lilium, interspecific crosses of L. × formolongi, L. formosanum, L. longiflorum (as seed parents) and BRO (as a pollen parent) were carried out. Capsules with unviable seeds were found in all crosses. To overcome the incongruity barrier, ovary slices excised from developing ovaries at 7 to 28 days after pollination (DAP) were cultured in Murashige and Skoog medium supplemented with 40 g·L−1 sucrose, 40 g·L−1 D-mannitol and 2.5 g·L−1 gellan gum under 24 h illumination. Hybrid plantlets were obtained from crosses of L. × formolongi × BRO and L. formosanum × BRO, but not from the cross of L. longiflorum × BRO. After acclimatization, 79 (44.1%) of 179 hybrid plants produced flowers within a year of cultivation. The hybrids developed flowers changing from yellowish cream to white during anthesis as does the pollen parent. Twenty-eight of 79 flowered plants sprouted two or more flower stalks, characteristics of L. formosanum. The hybrids exhibited relatively high pollen fertility and produced capsules, but no mature seed was obtained by self-pollination.

[1]  H. Okubo,et al.  Phylogenetic Relationship between Degree of Self-compatibility and Floral Traits in Lilium longiflorum Thunb. (Liliaceae) , 2012 .

[2]  Hiroshi Okubo,et al.  The global flower bulb industry: production, utilization, research. , 2010 .

[3]  H. Okubo,et al.  Interspecific lily hybrids with the ability to flower precociously and to produce multiple flower stalks from Lilium formosanum , 2008 .

[4]  H. Okubo.  History of Lilium brownii var. colchesteri in Japan. , 2006 .

[5]  K. Lim,et al.  Use of 2n Gametes for Inducing Intergenomic Recombination in Lily Hybrids , 2005 .

[6]  G. J. Dowrick,et al.  Abnormalities of endosperm development inLilium hybrids , 1970, Euphytica.

[7]  FukaiSeiichi FukaiSeiichi,et al.  Interspecific Hybrids between Lilium × formolongi and Some Asian Trumpet Species , 2004 .

[8]  K. Haghighi,et al.  Fertile, intermediate hybrids between Phaseolus vulgaris and P. acutifolius from congruity backcrossing , 1988, Sexual Plant Reproduction.

[9]  H. Okubo,et al.  Biogeography and origin of Lilium longiflorum and L. formosanum II - Intra- and interspecific variation in stem leaf morphology, flowering rate and individual net production during the first year seedling growth , 2002 .

[10]  Chi Wei Huang,et al.  Biogeography and origin of Lilium longiflorum and L. formosanum (Liliaceae) endemic to the Ryukyu Archipelago and Taiwan as determined by allozyme diversity. , 2001, American journal of botany.

[11]  T. Nagamine,et al.  Phylogenetic Analysis of Section Sinomartagon in Genus Lilium Using Sequences of the Internal Transcribed Spacer Region in Nuclear Ribosomal DNA , 2001 .

[12]  J. M. Tuyl,et al.  Application of four pollination techniques and of hormone treatment for bypassing interspecific crossing barriers in Lilium L. , 2000 .

[13]  E. Macrae.  Lilies: a guide for growers and collectors. , 1998 .

[14]  B. C. E. Ven,et al.  Interspecific lily hybrids: a promise for the future , 1997 .

[15]  A. M. Fernández,et al.  Development of diploid and triploid interspecific hybrids between Lilium longiflorum and L. concolor by ovary slice culture , 1996 .

[16]  H. Löffler,et al.  Segregation of Fusarium resistance in an interspecific cross between Lilium longiflorum and Lilium dauricum , 1996 .

[17]  T. Nikaido,et al.  Steroidal saponins from Smilax riparia and S. china. , 1992, Phytochemistry.

[18]  Y. Niimi,et al.  A Study on the Storage of Lilium Pollen , 1992 .

[19]  R. Bino,et al.  Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses , 1991 .

[20]  J. M. Tuyl,et al.  Research on polyploidy in interspecific hybridization of lily , 1990 .

[21]  L. Raamsdonk,et al.  Wide interspecific hybridization of Lilium: preliminary results of the application of pollination and embryo-rescue methods. , 1990 .

[22]  K. Kanoh,et al.  Production of Interspecific Hybrids between Lilium longiflorum and L.×elegance by Ovary Slice Culture , 1988 .

[23]  J. Franken,et al.  INTERSPECIFIC HYBRIDIZATION IN LILIUM , 1986 .

[24]  K. Kanoh,et al.  Ovary Slice Culture of Lilium formosanum WALLACE , 1986 .

[25]  Y. Asano.  Overcoming Interspecific Hybrid Sterility in Lilium , 1982 .

[26]  J. M. Tuyl.  Lily breeding research at IVT in Wageningen. , 1980 .

[27]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .