A high-performance micromixer using three-dimensional Tesla structures for bio-applications

Abstract Micromixers have been commonly utilized in the areas of biotechnical engineering, analytical chemistry, medical industry, and high-throughput synthesis because biological processes generally involve complicated chemical reactions that require useful reactant mixing for initiation. This paper reports the design, simulation, fabrication and test of a new high-performance micromixer via three-dimensional (3D) Tesla structures to realize the effective mixing process in the microfluidic devices for bio-applications. Both computational fluid dynamics (CFD) calculations and experiments were conducted to study the flow and mixing characteristics in the development of 3D Tesla micromixers. The predicted and measured results of the proposed micromixer have illustrated excellent mixing performance for Reynolds numbers (Re) ranging from 0.1 to 100 (0.015–15 μL/s) with its pressure drop less than 1054 Pa at Re = 100. We also showed the successful application of the proposed mixer to the immunofluorescence analysis of the EGF receptor L858R mutant specific rabbit mAb (as a cancer biomarker) recognized by anti-rabbit IgG-CFL555 and H1975 cells, revealing the mixing effectiveness of this microfluidic device in the binding reaction of antibodies for detecting antigens of lung cancer cellular surface.

[1]  Koji Sugioka,et al.  Rapid prototyping of three-dimensional microfluidic mixers in glass by femtosecond laser direct writing. , 2012, Lab on a chip.

[2]  Hamid Latifi,et al.  Simulation and experimental investigation of planar micromixers with short-mixing-length , 2013 .

[3]  A. Bhagat,et al.  Enhancing particle dispersion in a passive planar micromixer using rectangular obstacles , 2008 .

[4]  G. Whitesides,et al.  Microfabrication inside capillaries using multiphase laminar flow patterning , 1999, Science.

[5]  Adrian Wing-Keung Law,et al.  Measurement of mixing processes with combined digital particle image velocimetry and planar laser induced fluorescence , 2000 .

[6]  T. R. Shih,et al.  A high-efficiency planar micromixer with convection and diffusion mixing over a wide Reynolds number range , 2008 .

[7]  I. Mezić,et al.  Chaotic Mixer for Microchannels , 2002, Science.

[8]  Kwang-Yong Kim,et al.  Passive split and recombination micromixer with convergent–divergent walls , 2012 .

[9]  Jung Kyung Kim,et al.  Rapid three-dimensional passive rotation micromixer using the breakup process , 2004 .

[10]  Wolfgang Ehrfeld,et al.  Microreactors: New Technology for Modern Chemistry , 2000 .

[11]  Necati Kaval,et al.  Inertial microfluidics for sheath-less high-throughput flow cytometry , 2010, Biomedical microdevices.

[12]  J. P. V. Doormaal,et al.  ENHANCEMENTS OF THE SIMPLE METHOD FOR PREDICTING INCOMPRESSIBLE FLUID FLOWS , 1984 .

[13]  S. Acharya,et al.  Comparison of the Piso, Simpler, and Simplec Algorithms for the Treatment of the Pressure-Velocity Coupling in Steady Flow Problems , 1986 .

[14]  Jens Anders Branebjerg,et al.  Fast mixing by lamination , 1996, Proceedings of Ninth International Workshop on Micro Electromechanical Systems.

[15]  Zhengdong Wang,et al.  Design and evaluation of an easily fabricated micromixer with three-dimensional periodic perturbation , 2011 .

[16]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[17]  A Manz,et al.  Microfabricated devices for fluid mixing and their application for chemical synthesis. , 2001, Chemical record.

[18]  Kwang-Yong Kim,et al.  Analysis and optimization of a micromixer with a modified Tesla structure , 2010 .

[19]  Göran Stemme,et al.  A fast passive and planar liquid sample micromixer. , 2004, Lab on a chip.

[20]  Yong Jin,et al.  Visualization of micro-scale mixing in miscible liquids using μ-LIF technique and drug nano-particle preparation in T-shaped micro-channels , 2012 .

[21]  Erik T. K. Peterson,et al.  A passive planar micromixer with obstructions for mixing at low Reynolds numbers , 2007 .

[22]  F. Regnier,et al.  A picoliter-volume mixer for microfluidic analytical systems. , 2001, Analytical chemistry.

[23]  W. Malsche,et al.  Combined improved mixing and reduced energy dissipation by combining convective effects and lamination , 2012 .

[24]  Christopher J. Morris,et al.  Low-order modeling of resonance for fixed-valve micropumps based on first principles , 2003 .

[25]  K. Jensen,et al.  Design and fabrication of microfluidic devices for multiphase mixing and reaction , 2002 .

[26]  Alan G. R. Evans,et al.  Two simple micromixers based on silicon , 1998 .

[27]  Bernhard H. Weigl,et al.  Design and Rapid Prototyping of Thin-Film Laminate-Based Microfluidic Devices , 2001 .

[28]  Jin-Woo Choi,et al.  A novel in-plane passive microfluidic mixer with modified Tesla structures. , 2004, Lab on a chip.

[29]  Volker Hessel,et al.  Utilization of Micromixers for Extraction Processes , 2001 .

[30]  A. Manz,et al.  Miniaturized total chemical analysis systems: A novel concept for chemical sensing , 1990 .

[31]  Chienliu Chang,et al.  Experimental study of flow characteristics and mixing performance in a PZT self-pumping micromixer , 2007 .

[32]  Kazuhiro Mae,et al.  Design of confluence and bend geometry for rapid mixing in microchannels , 2013 .