Continuously phase-modulated standing surface acoustic waves for separation of particles and cells in microfluidic channels containing multiple pressure nodes

This paper describes continuously phase-modulated standing surface acoustic waves (CPM-SSAW) and its application for particle separation in multiple pressure nodes. A linear change of phase in CPM-SSAW applies a force to particles whose magnitude depends on their size and contrast factors. During continuous phase modulation, we demonstrate that particles with a target dimension are translated in the direction of moving pressure nodes, whereas smaller particles show oscillatory movements. The rate of phase modulation is optimized for separation of target particles from the relationship between mean particle velocity and period of oscillation. The developed technique is applied to separate particles of a target dimension from the particle mixture. Furthermore, we also demonstrate human keratinocyte cells can be separated in the cell and bead mixture. The separation technique is incorporated with a microfluidic channel spanning multiple pressure nodes, which is advantageous over separation in a single pressure node in terms of throughput.

[1]  Francesco Costanzo,et al.  Numerical study of acoustophoretic motion of particles in a PDMS microchannel driven by surface acoustic waves. , 2015, Lab on a chip.

[2]  David J. Collins,et al.  Batch process particle separation using surface acoustic waves (SAW): integration of travelling and standing SAW , 2016 .

[3]  Pierre Thibault,et al.  Fast acoustic tweezers for the two-dimensional manipulation of individual particles in microfluidic channels , 2012, 1211.2967.

[4]  Andrew G. Glen,et al.  APPL , 2001 .

[5]  Henrik Bruus,et al.  Acoustofluidics 7: The acoustic radiation force on small particles. , 2012, Lab on a chip.

[6]  Henrik Bruus,et al.  Acoustofluidics 1: Governing equations in microfluidics. , 2011, Lab on a chip.

[7]  R. W. Rambach,et al.  Surface acoustic wave controlled integrated band-pass filter , 2015 .

[8]  Fei Yan,et al.  Transportation of single cell and microbubbles by phase-shift introduced to standing leaky surface acoustic waves. , 2011, Biomicrofluidics.

[9]  Tony Jun Huang,et al.  A high-throughput acoustic cell sorter. , 2015, Lab on a chip.

[10]  James Friend,et al.  Direct visualization of surface acoustic waves along substrates using smoke particles , 2007 .

[11]  T. Huang,et al.  Acoustic separation of circulating tumor cells , 2015, Proceedings of the National Academy of Sciences.

[12]  Hakho Lee,et al.  Acoustic purification of extracellular microvesicles. , 2015, ACS nano.

[13]  Hyung Jin Sung,et al.  Acoustothermal heating of polydimethylsiloxane microfluidic system , 2015, Scientific Reports.

[14]  Jin Ho Jung,et al.  Microchannel anechoic corner for size-selective separation and medium exchange via traveling surface acoustic waves. , 2015, Analytical chemistry.

[15]  Sehyun Shin,et al.  Separation of platelets from whole blood using standing surface acoustic waves in a microchannel. , 2011, Lab on a chip.

[16]  P. Sajeesh,et al.  Particle separation and sorting in microfluidic devices: a review , 2014 .

[17]  Rasim Guldiken,et al.  Active density-based separation using standing surface acoustic waves , 2012 .

[18]  Thomas Laurell,et al.  Continuous separation of cells and particles in microfluidic systems. , 2010, Chemical Society reviews.

[19]  J. P. McCoy,et al.  Acoustofluidic Fluorescence Activated Cell Sorter. , 2015, Analytical chemistry.

[20]  Peng Li,et al.  Surface acoustic wave microfluidics. , 2013, Lab on a chip.

[21]  D. Ingber,et al.  Micromagnetic-microfluidic blood cleansing device. , 2009, Lab on a chip.

[22]  Anas Alazzam,et al.  Continuous separation of particles in a PDMS microfluidic channel via travelling surface acoustic waves (TSAW). , 2013, Lab on a chip.