New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. II. Inference of the precisely timed control system regulating glycolysis.

The dairy bacterium Lactococcus lactis has to master a complicated task. It must control its essentially linear glycolytic pathway in such a fashion that, when the substrate, glucose, runs out, it retains enough phosphoenolpyruvate and fructose-1,6-bisphosphate to be able to restart glycolysis as soon as new glucose becomes available. Although glycolysis is arguably the best-studied metabolic pathway, its details in L. lactis are still unclear, and it is, in particular, not understood how the bacterium manages the stop-and-start task. The primary purpose of this paper and its companion is a clarification of some of the details of the governing regulatory strategies with which L. lactis manages to retain the necessary metabolites for the restart of glycolysis after periods of starvation. The paper furthermore discusses how the bacterium changes these strategies when it is subjected to aerobic rather than anaerobic conditions.