Determination of Cyanogenic Compounds in Edible Plants by Ion Chromatography

Cyanogenic glycosides are HCN-producing phytotoxins; HCN is a powerful and a rapidly acting poison. It is not difficult to find plants containing these compounds in the food supply and/or in medicinal herb collections. The objective of this study was to investigate the distribution of total cyanide in nine genera (Dolichos, Ginkgo, Hordeum, Linum, Phaseolus, Prunus, Phyllostachys, Phytolacca , and Portulaca) of edible plants and the effect of the processing on cyanide concentration. Total cyanide content was measured by ion chromatography following acid hydrolysis and distillation. Kernels of Prunus genus are used medicinally, but they possess the highest level of total cyanide of up to 2259.81 CN7g dry weight. Trace amounts of cyanogenic compounds were detected in foodstuffs such as mungbeans and bamboo shoots. Currently, except for the WHO guideline for cassava, there is no global standard for the allowed amount of cyanogenic compounds in foodstuffs. However, our data emphasize the need for the guidelines if plants containing cyanogenic glycosidesare to be developed as dietary supplements.

[1]  J. Howard Bradbury,et al.  Processing of cassava roots to remove cyanogens , 2005 .

[2]  R. Fall,et al.  Simultaneous determination of cyanide and carbonyls in cyanogenic plants by gas chromatography-electron capture/photoionization detection. , 2002, The Analyst.

[3]  N. Grané-Teruel,et al.  Chromatographic determination of cyanoglycosides prunasin and amygdalin in plant extracts using a porous graphitic carbon column. , 2002, Journal of Agricultural and Food Chemistry.

[4]  J. Howard Bradbury,et al.  Total cyanide determination of plants and foods using the picrate and acid hydrolysis methods , 2002 .

[5]  J. Bradbury,et al.  Picrate paper kits for determination of total cyanogens in cassava roots and all forms of cyanogens in cassava products , 1999 .

[6]  G. Mazza,et al.  Determination of Cyanogenic Glycosides in Flaxseed by Barbituric Acid−Pyridine, Pyridine−Pyrazolone, and High-Performance Liquid Chromatography Methods† , 1996 .

[7]  D. Seigler,et al.  VARIATION OF CYANOGENESIS IN SOME PLANT SPECIES OF THE MIDWESTERN UNITED STATES , 1996 .

[8]  J. Bradbury,et al.  COMPARISON OF METHODS OF ANALYSIS OF CYANOGENS IN CASSAVA , 1994 .

[9]  T. Shibamoto,et al.  Introduction to Food Toxicology , 1993 .

[10]  G. Mazza,et al.  Cyanogenic compounds in flaxseed , 1992 .

[11]  M. Pinotti,et al.  Cyanogenic glycosides in plants , 2000 .

[12]  J. Vetter.  Plant cyanogenic glycosides. , 2000, Toxicon : official journal of the International Society on Toxinology.

[13]  H. Yeoh,et al.  Simple Picrate Paper Kit for Determination of the Cyanogenic Potential of Cassava Flour , 1998 .

[14]  J. Bradbury,et al.  Analysis of cyanide in cassava using acid hydrolysis of cyanogenic glucosides , 1991 .