Hydrogen Donors for Reduction of Lipid Hydroperoxides with Heme-proteins

by horseradish peroxidase in the presence of a dye in the leuco form.3) Hemoglobin4'5) was measured using the formation of hematin, which catalyzes the conversion of hydrogen peroxide to water and oxygen. The measurement of lipid hydroperoxides with hemoglobin was also reported.6) Peroxidation reaction of metmyoglobin and metemoglobin has been extensively studied.7) Hemoglobin activity as a peroxidase was tested to oxidize thiocyanate.8) Cavallini et al.9) reported that NADH and NADPHinhibited rat liver microsomal lipid peroxidation promoted by hydroperoxides. They suggested that this may involve a reduction of the hydroperoxides mediated by cytochrome P-450. Lindstrom and Aust10) have