Pineal Gland Free Amino Acids and Indoles During Postnatal Development of the Rat: Correlations in Individual Glands

Free amino acids and indoles were measured by HPLC in single pineal glands of 5‐, 10‐and 20‐day‐old rats sacrificed during mid‐light and mid‐dark at each age. Melatonin was detectable in neonates (5‐day‐old), but day vs night differences in indole constituents did not occur until 10 days of age. Free amino acid steady state levels were high in neonates and there was a tendency for reversal of day vs night differences coinciding with the onset of circadian rhythmicity in indole biosynthesis. High correlations (r > 0.85) existed for taurine vs. glutamate in individual glands regardless of age and time of sacrifice. These findings suggest that taurine and glutamate are biochemically interrelated and that developmental changes in amino acid metabolic pools reflect functional innervation of the gland.

[1]  M. Ebadi,et al.  The inhibition of pineal arylalkylamine n-acetyltransferase by glutamic acid and its analogues , 1988, Neurochemistry International.

[2]  J. Sturman.  Taurine in development. , 1988, The Journal of nutrition.

[3]  S. Lisco,et al.  Pinealocyte Dense‐Cored Vesicles and Synaptic Ribbons: A Correlative Ultrastructural‐Biochemical Investigation in Rats and Mice , 1987, Journal of pineal research.

[4]  Rand R. Wilcox,et al.  New designs in analysis of variance. , 1987 .

[5]  R. Matucci,et al.  The effect of taurine on excitatory receptor binding in rat hippocampal membranes. , 1987, Advances in experimental medicine and biology.

[6]  P. Foley,et al.  Pineal indoles: Significance and measurement , 1986, Neuroscience & Biobehavioral Reviews.

[7]  W. Simmons,et al.  Correlations of serotonin and its metabolites in individual rat pineal glands over light:dark cycles and after acute light exposure. , 1986, Life sciences.

[8]  M. Ebadi,et al.  Identification of a Cl−/Ca2+‐Dependent Glutamate (Quisqualate) Binding Site in Bovine Pineal Organ , 1986, Journal of pineal research.

[9]  H. Aihara,et al.  The action of taurine on the response to glutamate in the motoneuron of the isolated frog spinal cord , 1985, Neuropharmacology.

[10]  H. Meissl,et al.  Electrophysiological studies on neuronal transmission in the frog's photosensory pineal organ The effect of amino acids and biogenic amines , 1984, Vision Research.

[11]  J. Swedlow,et al.  Pineal N-acetyltransferase activity in 10-day-old rats: a paradigm for studying the developing circadian system. , 1984, Endocrinology.

[12]  H. Meissl,et al.  Photosensory properties of the pineal organ. Microiontophoretic application of excitatory amino acids onto pineal neurons. , 1984, Ophthalmic research.

[13]  R. Reiter,et al.  Concurrent determination of enzymatic activities and substrate concentrations in the melatonin synthetic pathway within the same rat pineal gland , 1984, Journal of neuroscience research.

[14]  D. Klein,et al.  Taurine in the pineal gland. , 1983, Progress in clinical and biological research.

[15]  K. Yoshihara,et al.  Effect of taurine on depolarizations induced by L-glutamate and other excitatory amino acids in the isolated spinal cord of the frog. , 1983, Japanese journal of pharmacology.

[16]  A. Patel,et al.  Effect of Age on Variables Influencing the Supply of Tryptophan to the Brain , 1982, Journal of neurochemistry.

[17]  A. Altar.  Development of the mammalian pineal gland. , 1982, Developmental neuroscience.

[18]  N. V. van Gelder.  Changed taurine-glutamic acid content and altered nervous tissue cytoarchitecture. , 1981, Advances in experimental medicine and biology.

[19]  D. Rassin.  Taurine, cysteinesulfinic acid decarboxylase and glutamic acid in brain. , 1981, Advances in experimental medicine and biology.

[20]  D. Klein,et al.  Taurine release from the pineal gland is stimulated via a β-adrenergic mechanism , 1980, Brain Research.

[21]  D. Klein,et al.  Taurine: Stimulation of pineal N-acetyltransferase activity and melatonin production via a beta-adrenergic mechanism , 1979, Brain Research.

[22]  V. Neuhoff,et al.  Pineal proteins and free amino acids during ontogenesis in rats. , 1974, Neuroendocrinology.

[23]  V. Neuhoff,et al.  Rat pineal free amino acids diurnal rhythm and effect of light. , 1973, Archives internationales de physiologie et de biochimie.

[24]  H. Agrawal,et al.  POSTNATAL CHANGES IN FREE AMINO ACID POOL OF RAT BRAIN , 1966, Journal of neurochemistry.