Determination of fumonisins B1 and B2 in maize-based baby food products by HPLC with fluorimetric detection after immunoaffinity column clean-up.

A method for the determination of fumonisin B1 (FB1) and B2 (FB2) in different commercial maize-based products for infants and young children was developed and tested in a limited validation study involving 3 laboratories. The method used extraction at 55 °C with an acidic mixture of methanol-acetonitrile-phosphate/citrate buffer, clean-up through immunoaffinity column and fumonisin determination by high performance liquid chromatography with automated pre-column derivatisation with o-phthaldialdehyde. Recovery experiments were performed at five spiking levels in the ranges of 80-800 µg/kg FB1 and 20-200 µg/kg FB2. Mean recoveries ranged from 83 to 97% for FB1 and from 61 to 78% for FB2. Relative standard deviations for within-laboratory repeatability (RSDr) ranged from 5 to 12% for FB1 and from 8 to 13% for FB2, whereas relative standard deviation for between-laboratory reproducibility (RSDR) ranged from 6 to 10% for FB1 and from 9 to 16% for FB2. The limit of quantification of the method (signal to nois...

[1]  G. Font,et al.  Survey of fumonisins B1, B2 and B3 in conventional and organic retail corn products in Spain and Italy and estimated dietary exposure , 2009, Food additives & contaminants. Part B, Surveillance.

[2]  R. Marchelli,et al.  Difficulties in fumonisin determination: the issue of hidden fumonisins , 2009, Analytical and bioanalytical chemistry.

[3]  S. Chung,et al.  Incomplete recoveries of fumonisins present in naturally contaminated corn foods from an immunoaffinity column. , 2009, Journal of AOAC International.

[4]  G. Font,et al.  Analysis of fumonisins B(1), B(2) and B(3) in corn-based baby food by pressurized liquid extraction and liquid chromatography/tandem mass spectrometry. , 2008, Journal of chromatography. A.

[5]  M. Hanna,et al.  Reduction of fumonisin B1 in corn grits by single-screw extrusion. , 2008, Journal of agricultural and food chemistry.

[6]  P. Scott,et al.  Studies on extraction of fumonisins from rice, corn-based foods and beans , 1999, Mycotoxin Research.

[7]  S. van Calenbergh,et al.  Development of a liquid chromatography/tandem mass spectrometry method for the quantification of fumonisin B1, B2 and B3 in cornflakes. , 2005, Rapid communications in mass spectrometry : RCM.

[8]  R. Schothorst,et al.  Report from SCOOP task 3.2.10 "collection of occurrence data of Fusarium toxins in food and assessment of dietary intake by the population of EU member states". Subtask: trichothecenes. , 2004, Toxicology letters.

[9]  H. Humpf,et al.  Effects of thermal food processing on the chemical structure and toxicity of fumonisin mycotoxins. , 2004, Molecular nutrition & food research.

[10]  G. Shephard,et al.  Fumonisins in Brazilian corn-based foods for infant consumption , 2004, Food additives and contaminants.

[11]  F. Galvano,et al.  Natural co-occurrence of deoxynivalenol and fumonisins B1 and B2 in Italian marketed foodstuffs , 2003, Food additives and contaminants.

[12]  W. Marasas,et al.  Improving extraction of fumonisin mycotoxins from Brazilian corn-based infant foods. , 2003, Journal of food protection.

[13]  P. Scott,et al.  Mycotoxins in infant cereal foods from the Canadian retail market , 2003, Food additives and contaminants.

[14]  B. Lau,et al.  Extraction of fumonisins B1 and B2 from white rice flour and their stability in white rice flour, cornstarch, cornmeal, and glucose. , 2002, Journal of agricultural and food chemistry.

[15]  A. De Girolamo,et al.  Determination of fumonisins B1 and B2 in corn and corn flakes by liquid chromatography with immunoaffinity column cleanup: collaborative study. , 2001, Journal of AOAC International.

[16]  A. De Girolamo,et al.  Determination of fumonisins B1 and B2 in cornflakes by high performance liquid chromatography and immunoaffinity clean-up , 2001, Food additives and contaminants.

[17]  A. De Girolamo,et al.  Comparison of different extraction and clean-up procedures for the determination of fumonisins in maize and maizebased food products , 2001, Food additives and contaminants.

[18]  M. Machinski Junior,et al.  Fumonisins B1 and B2 in Brazilian corn-based food products. , 2000, Food additives and contaminants.

[19]  J. F. Lawrence,et al.  Effect of temperature and solvent composition on extraction of fumonisins B1 and B2 from corn products. , 2000, Journal of AOAC International.

[20]  L. M. Valente Soares,et al.  Fumonisin levels in commercial corn products in Buenos Aires, Argentina , 2000, Food additives and contaminants.

[21]  P. Schreier,et al.  Identification and determination of fumonisin FB1 and FB2 in corn and corn products by high-performance liquid chromatography-electrospray-ionization tandem mass spectrometry (HPLC-ESI-MS-MS) , 1996 .

[22]  P. G. Thiel,et al.  Liquid chromatographic determination of fumonisins B1, B2, and B3 in corn: AOAC-IUPAC Collaborative Study. , 1996, Journal of AOAC International.

[23]  M. Trucksess,et al.  Immunoaffinity column coupled with liquid chromatography for determination of fumonisin B1 in canned and frozen sweet corn. , 1995, Journal of AOAC International.

[24]  P. Scott,et al.  Stability and problems in recovery of fumonisins added to corn-based foods. , 1994, Journal of AOAC International.