Effect of Sodium Chloride on Uptake of Substrate by Staphylococcus aureus 196E.

Sodium chloride inhibited a number of biochemical parameters in Staphylococcus aureus 196E. Induction of phospho-β-galactosidase, synthesis of staphylococcal enterotoxin A, enzyme activity (phospho-β-galactosidase) and glucose utilization were approximately four times more sensitive to the inhibitory effects of salt than was growth. Uptake of 14C-2-deoxyglucose and respiratory activity with a number of substrates were inhibited also. The breakdown of o-nitrophenyl-β-galactoside (ONPG) by lactose-grown S. aureus 196E was inhibited by NaCl as well as by other solutes (salts, carbohydrates, amino acids) which suggested that the inhibitory effect is a general one of solutes and not restricted to NaCl. Various ionophores (gramicidin, valinomycin, monensin, lasalocid, m-chlorophenylhydrazone), the H+-ATPase inhibitor (N,N',-dicyclohexylcarbodiimide), and ion channel blockers (quinine, quinidine, chlorpromazinc, tetracaine, verapamil) reversed the inhibitory action of salt on ONPG breakdown by lactose-grown cells; however, these compounds did not reverse NaCl inhibition of glucose utilization. The effects observed here suggest that NaCl (and probably other solutes) exerts an inhibitory effect on transport of substrates into the cells of S. aureus 196E.

[1]  N. Uden,et al.  Transport of fructose by a proton symport in a brewing yeast , 1986 .

[2]  R. Vink,et al.  Regulation of the glucose phosphotransferase system in Brochothrix thermosphacta by membrane energization , 1985, Journal of bacteriology.

[3]  H. Morris,et al.  Galactose transport in Streptococcus thermophilus , 1985, Applied and environmental microbiology.

[4]  JAMES L. Smith,et al.  A REVIEW OF METHODS FOR DETECTION OF STAPHYLOCOCCAL ENTEROTOXINS AND EVALUATION OF AN ENZYME‐LINKED IMMUNOSORBENT ASSAY APPLIED TO FOODS , 1985 .

[5]  C. Deutsch,et al.  Osmotic effects on bacterial transport and energetics , 1985, FEBS letters.

[6]  E. Kopetzki,et al.  The Use of Phenolic Glycosides for Studying the Aerobic or Anaerobic Transport of Disaccharides into Yeasts , 1984 .

[7]  J. Adams,et al.  Thermocouple grounding in retort pouches , 1984 .

[8]  J. Sofos.  ANTIMICROBIAL EFFECTS OF SODIUM AND OTHER IONS IN FOODS: A REVIEW , 1984 .

[9]  M. Kitada,et al.  Energy Coupling Reactions of the Active Transport System for α-Aminoisobutyric Acid in Alkalophilic Bacillus no. 8–1 , 1982 .

[10]  P. Marsh,et al.  Influence of Sodium and Potassium Ions on Acid Production by Washed Cells of Streptococcus mutans Ingbritt and Streptococcus sanguis NCTC 7865 Grown in a Chemostat , 1982, Infection and immunity.

[11]  J. Thompson.  Galactose transport systems in Streptococcus lactis , 1980, Journal of bacteriology.

[12]  J. Troller,et al.  Water Activity and Food , 1978 .

[13]  J. Troller,et al.  Influence of water activity on the production of extracellular enzymes by Staphylococcus aureus , 1978, Applied and environmental microbiology.

[14]  B. Prior.  The effect of water activity on the growth and respiration of Pseudomonas fluorescens. , 1978, The Journal of applied bacteriology.

[15]  T. R. Dutson,et al.  EFFECT OF THERMOCOUPLE WIRE SIZE ON THE COOKING TIMES OF MEAT SAMPLES , 1977 .

[16]  W. Shafer,et al.  Regulation of staphylococcal enterotoxin B , 1977, Infection and immunity.

[17]  J. Gamlen,et al.  CALCULATION OF TEMPERATURE MEASUREMENT ERRORS IN THERMOCOUPLES IN CONVECTION HEATING CANS , 1974 .

[18]  A. Singh,et al.  Effect of Dicyclohexylcarbodiimide on Growth and Membrane-Mediated Processes in Wild Type and Heptose-Deficient Mutants of Escherichia coli K-12 , 1974, Journal of bacteriology.

[19]  C. Genigeorgis,et al.  Effect of sodium chloride and pH on enterotoxin C production. , 1971, Applied microbiology.

[20]  G. Silverman,et al.  Factors affecting the secretion of staphylococcal enterotoxin A. , 1970, Applied microbiology.

[21]  J. A. Alford,et al.  Effects of Meat-curing Salts and Temperature on Production of Staphylococcal Enterotoxin B , 1968, Journal of bacteriology.

[22]  E. P. Kennedy,et al.  Mechanism of hydrolysis of O-nitrophenyl-beta-galactoside in Staphylococcus aureus and its significance for theories of sugar transport. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. Middlehurst,et al.  Electrochemical effects in thermocouples , 1964 .