The photogenotoxicity of titanium dioxide particles.

We employed a series of in vitro genotoxicity assays--a single cell gel (SCG) assay with mouse lymphoma L5178Y cells, a microbial mutation assay with Salmonella typhimurium, a mammalian cell mutation assay with L5178Y cells, and a chromosomal aberration assay with Chinese hamster CHL/IU cells--to evaluate the photogenotoxicity of titanium dioxide (TiO2) particles. Without UV/visible light irradiation, TiO2 particles exhibited no or weak genotoxicity. With irradiation, however, TiO2 particles exhibited significant genotoxicity in the SCG and chromosomal aberration assays. Therefore, we concluded that TiO2 particles are photogenotoxic.

[1]  M Liebsch,et al.  EEC/COLIPA project on in vitro phototoxicity testing: First results obtained with a Balb/c 3T3 cell phototoxicity assay. , 1994, Toxicology in vitro : an international journal published in association with BIBRA.

[2]  A. Fujishima,et al.  Induction of cytotoxicity by photoexcited TiO2 particles. , 1992, Cancer research.

[3]  Z. Zainal,et al.  Bactericidal Activity of TiO2 Photocatalyst in Aqueous Media: Toward a Solar-Assisted Water Disinfection System. , 1994, Environmental science & technology.

[4]  M. Fujihira,et al.  Heterogeneous photocatalytic oxidation of aromatic compounds on TiO2 , 1981, Nature.

[5]  A. Fujishima,et al.  Photokilling of T-24 human bladder cancer cells with titanium dioxide. , 1994, British Journal of Cancer.

[6]  B. Bernard,et al.  Toxicology and carcinogenesis studies of dietary titanium dioxide-coated mica in male and female Fischer 344 rats. , 1989, Journal of toxicology and environmental health.

[7]  M. Shelby,et al.  Evaluation of a three‐exposure mouse bone marrow micronucleus protocol: Results with 49 chemicals , 1993, Environmental and molecular mutagenesis.

[8]  E. Gocke,et al.  Evaluation of the micronucleus test in vitro using chinese hamster cells: Results of four chemicals weakly positive in the in vivo micronucleus test , 1995, Environmental and molecular mutagenesis.

[9]  R. Tice,et al.  A simple technique for quantitation of low levels of DNA damage in individual cells. , 1988, Experimental cell research.

[10]  C. Dawson,et al.  Hydroxyl radical production and lung injury in the rat following silica or titanium dioxide instillation in vivo. , 1995, American journal of respiratory cell and molecular biology.

[11]  K. P. Lee,et al.  Pulmonary response of rats exposed to titanium dioxide (TiO2) by inhalation for two years. , 1985, Toxicology and applied pharmacology.

[12]  Ebtisam Wilkins,et al.  Photocatalytically‐mediated disinfection of water using tio2 as a catalyst and spore‐forming Bacillus pumilus as a model , 1995 .

[13]  B. Ames,et al.  Revised methods for the Salmonella mutagenicity test. , 1983, Mutation research.

[14]  B H Margolin,et al.  Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays. , 1987, Science.

[15]  G. Speit,et al.  Comparative investigations of the genotoxic effects of metals in the single cell gel (SCG) assay and the sister chromatid exchange (SCE) test , 1994, Environmental and molecular mutagenesis.

[16]  W. Caspary,et al.  Chemical mutagenesis at the thymidine kinase locus in L5178Y mouse lymphoma cells: Results for 31 coded compounds in the national toxicology program , 1991, Environmental and molecular mutagenesis.

[17]  G. M. Ridder,et al.  Pulmonary response to silica or titanium dioxide: inflammatory cells, alveolar macrophage-derived cytokines, and histopathology. , 1990, American journal of respiratory cell and molecular biology.