Metal accumulation and transport in the ovary of the lizard Podarcis sicula

Abstract The molecular characterisation of a CTR protein is reported in the ovary of the lizard Podarcis sicula; this protein proves to be homologous with the mammalian high affinity copper transporter CTRL Gene expression assessed by Northern blot hybridisation of RNA from growing ovarian follicles and eggs demonstrated that the transcript accumulated during the oocyte growth and reached the highest levels in ovulated eggs. Analysis of the copper content paralleled the profile of CTR1 mRNA, with a rapid increase of the metal concentration during oocyte maturation. These data suggest that the P. sicula CTR1 protein may function in the uptake and storage of the copper to be used during embryonic development.

[1]  D. Thiele,et al.  Characterization of Mouse Embryonic Cells Deficient in the Ctr1 High Affinity Copper Transporter , 2002, The Journal of Biological Chemistry.

[2]  Liping Huang,et al.  Functional Characterization of a Novel Mammalian Zinc Transporter, ZnT6* , 2002, The Journal of Biological Chemistry.

[3]  D. Thiele,et al.  High affinity copper transport protein in the lizard Podarcis sicula: molecular cloning, functional characterization and expression in somatic tissues, follicular oocytes and eggs. , 2002, Biochimica et biophysica acta.

[4]  T. Iwanaga,et al.  Cloning and Characterization of a Novel Mammalian Zinc Transporter, Zinc Transporter 5, Abundantly Expressed in Pancreatic β Cells* , 2002, The Journal of Biological Chemistry.

[5]  H. Haase,et al.  Functions of zinc in signaling, proliferation and differentiation of mammalian cells , 2001, Biometals.

[6]  Dominique L. Cosco,et al.  The copper transporter CTR1 provides an essential function in mammalian embryonic development , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[7]  D. Thiele,et al.  Essential role for mammalian copper transporter Ctr1 in copper homeostasis and embryonic development , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[8]  D. Thiele,et al.  Isolation of a murine copper transporter gene, tissue specific expression and functional complementation of a yeast copper transport mutant. , 2000, Gene.

[9]  R. Palmiter,et al.  Seizures and neuronal damage in mice lacking vesicular zinc , 2000, Epilepsy Research.

[10]  D. Thiele,et al.  A Copper-sensing Transcription Factor Regulates Iron Uptake Genes in Schizosaccharomyces pombe * , 1999, The Journal of Biological Chemistry.

[11]  D. Thiele,et al.  A delicate balance: homeostatic control of copper uptake and distribution. , 1999, The Journal of nutrition.

[12]  Kenneth H. Falchuk,et al.  The molecular basis for the role of zinc in developmental biology , 1998, Molecular and Cellular Biochemistry.

[13]  D. Thiele,et al.  Dynamic Regulation of Copper Uptake and Detoxification Genes in Saccharomyces cerevisiae , 1998, Molecular and Cellular Biology.

[14]  Bing Zhou,et al.  hCTR1: a human gene for copper uptake identified by complementation in yeast. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[15]  D. Eide,et al.  The ZRT2 Gene Encodes the Low Affinity Zinc Transporter in Saccharomyces cerevisiae* , 1996, The Journal of Biological Chemistry.

[16]  M. Linder,et al.  Copper biochemistry and molecular biology. , 1996, The American journal of clinical nutrition.

[17]  D. Eide,et al.  The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[18]  W. Kunz,et al.  Increase of Flux Control of Cytochrome c Oxidase in Copper-deficient Mottled Brindled Mice (*) , 1996, The Journal of Biological Chemistry.

[19]  J. Prohaska,et al.  Alterations of Rat Brain Peptidylglycine α-Amidating Monooxygenase and Other Cuproenzyme Activities Following Perinatal Copper Deficiency , 1995, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[20]  A. Willems,et al.  Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure , 1995, Yeast.

[21]  R. Müller,et al.  Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. , 1995, Gene.

[22]  R. Palmiter,et al.  Cloning and functional characterization of a mammalian zinc transporter that confers resistance to zinc. , 1995, The EMBO journal.

[23]  B. Vallee,et al.  Zinc, iron, and copper contents of Xenopus laevis oocytes and embryos , 1993, Molecular reproduction and development.

[24]  W. Schumm,et al.  An Exploratory Study of Homeschooling in Kansas , 1993 .

[25]  M. Linder.  Biochemistry of Copper , 1991, Biochemistry of the Elements.

[26]  B. Vallee,et al.  Histone formation, gene expression, and zinc deficiency in Euglena gracilis. , 1984, Biochemistry.

[27]  C. Keen,et al.  Comparative aspects of dietary copper and zinc deficiencies in pregnant rats. , 1983, The Journal of nutrition.

[28]  M. Kirchgessner,et al.  [Contents and distribution of Fe, Cu, Zn, Ni, and Mn in fetuses, amniotic fluid, placenta, and uterus of rats]. , 1982, Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie.

[29]  M. Kirchgessner,et al.  Gehalte und Verteilung von Fe, Cu, Zn, Ni und Mn in Foeten, Fruchtwasser, Placenta und Uterus von Ratten , 1982 .

[30]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[31]  T. Iwanaga,et al.  Cloning and characterization of a novel mammalian zinc transporter, zinc transporter 5, abundantly expressed in pancreatic beta cells. , 2002, The Journal of biological chemistry.

[32]  B. Vallee,et al.  The biochemical basis of zinc physiology. , 1993, Physiological reviews.

[33]  C. Keen,et al.  Zinc and Reproduction: Effects of Deficiency on Foetal and Postnatal Development , 1989 .