Phagocytosis, bacterial killing, and metabolism by purified human lung phagocytes.

Phagocytic and bactericidal activities of purified polymorphonuclear neutrophils (PMNs), monocytes (MNs), or alveolar macrophages (AMs) from the same individuals for Staphylococcus aureus strain 502A or Escherichia coli strain ON2 were compared in vitro. Factors that may influence these activities were evaluated. The bactericidal activity of S. aureus and E. coli of PMNs was greater than that of MNs or AMs. Two factors were identified that may account for this result. (1) The rate of phagocytosis of opsonized S. aureus and E. coli was greater by PMNs. (2) The nonmitochondrial oxygen burst after nonparticulate stimulation was much greater by PMNs. Qualitative differences in opsonic requirements for ingestion of S. aureus by PMNs, MNs, and AMs were also identified. In contrast to PMNs or MNs, phagocytosis of S. aureus by AMs was not opsonin-dependent. The results suggest that mononuclear phagocytes may adapt their opsonic requirements to facilitate uptake of S. aureus. Despite this adaptation, their bactericidal capability remains limited when compared with PMNs.

[1]  R. Reynolds,et al.  Leukocytic response to inhaled bacteria. , 2015, The American review of respiratory disease.

[2]  A. Schreiber,et al.  The role of antibody and complement in phagocytosis by rabbit alveolar macrophages. , 1979, The Journal of infectious diseases.

[3]  J. Repine,et al.  Defective oxidative metabolic responses in vitro of alveolar macrophages in chronic granulomatous disease. , 1979, The American review of respiratory disease.

[4]  D. Segal,et al.  Human leukocyte Fc (IgG) receptors: quantitation and affinity with radiolabeled affinity cross-linked rabbit IgG. , 1979, Journal of immunology.

[5]  R. Furth,et al.  Origin, Kinetics, and characteristics of pulmonary macrophages in the normal steady state , 1979, The Journal of experimental medicine.

[6]  J. White,et al.  Influence of cationic local anesthetics on the metabolism and ultrastructure of human alveolar macrophages. , 1979, The Journal of laboratory and clinical medicine.

[7]  J. White,et al.  The effect of phorbol myristate acetate on the metabolism and ultrastructure of human alveolar macrophages. , 1978, The American journal of pathology.

[8]  T. Stossel,et al.  Complement (C3)-activated phagocytosis by lung macrophages. , 1978, Journal of immunology.

[9]  S. Douglas,et al.  Purification of human monocytes on microexudate-coated surfaces. , 1978, Journal of immunology.

[10]  D. Roos,et al.  Differences in oxygen metabolism of phagocytosing monocytes and neutrophils. , 1978, The Journal of clinical investigation.

[11]  J. Verhoef,et al.  Kinetics of phagocytosis and bacterial killing by human polymorphonuclear leukocytes and monocytes. , 1977, The Journal of infectious diseases.

[12]  J. Verhoef,et al.  Effect of protein A on staphylococcal opsonization , 1977, Infection and immunity.

[13]  G. S. Davis,et al.  Defense mechanisms of the respiratory membrane. , 1977, The American review of respiratory disease.

[14]  J. Verhoef,et al.  Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria. , 1977, Journal of immunological methods.

[15]  L. Mcphail,et al.  Further characterization of NADPH oxidase activity of human polymorphonuclear leukocytes. , 1976, The Journal of clinical investigation.

[16]  William W. Cook,et al.  Enhancement of bactericidal capacity of alveolar macrophages by human alveolar lining material. , 1976, The Journal of clinical investigation.

[17]  R. Johnston,et al.  Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G , 1976, The Journal of experimental medicine.

[18]  H. Reynolds,et al.  Specificity of opsonic antibodies to enhance phagocytosis of Pseudomonas aeruginosa by human alveolar macrophages. , 1975, The Journal of clinical investigation.

[19]  S. Klebanoff.  Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. , 1975, Seminars in hematology.

[20]  A. Deinard,et al.  Metabolic and Functional Studies of Monocytes in Congenital Neutropenia , 1974, British journal of haematology.

[21]  H. Reynolds,et al.  Analysis of proteins and respiratory cells obtained from human lungs by bronchial lavage. , 1974, The Journal of laboratory and clinical medicine.

[22]  E. Goldstein,et al.  Pulmonary alveolar macrophage. Defender against bacterial infection of the lung. , 1974, The Journal of clinical investigation.

[23]  J. White,et al.  Effects of phorbol myristate acetate on the metabolism and ultrastructure of neutrophils in chronic granulomatous disease. , 1974, Journal of Clinical Investigation.

[24]  G. Huber,et al.  Inactivation of staphylococci by alveolar macrophages with preliminary observations on the importance of alveolar lining material. , 1973, The American review of respiratory disease.

[25]  G. Poste,et al.  The study of cellular "microexudates" by ellipsometry and their relationship to the cell coat. , 1973, Experimental cell research.

[26]  C. Li,et al.  ESTERASES IN HUMAN LEUKOCYTES , 1973, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[27]  R. Johnston,et al.  Monocyte function in children with neutropenia and chronic infections. , 1972, Blood.

[28]  A. Cohen,et al.  The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics. , 1971, The Journal of clinical investigation.

[29]  G. Youmans,et al.  Demonstration in Tissue Culture of Lymphocyte-Mediated Immunity to Tuberculosis , 1970, Infection and immunity.

[30]  R. Lehrer,et al.  Leukocyte myeloperoxidase deficiency and disseminated candidiasis: the role of myeloperoxidase in resistance to Candida infection. , 1969, The Journal of clinical investigation.

[31]  R. Lehrer,et al.  Phagocytosis by human monocytes. , 1968, Blood.

[32]  J. Worcester.  The statistical method. , 1966, The New England journal of medicine.

[33]  E. Kass,et al.  THE ROLE OF THE ALVEOLAR MACROPHAGE IN THE CLEARANCE OF BACTERIA FROM THE LUNG , 1964, The Journal of experimental medicine.

[34]  W. Wood,et al.  STUDIES ON THE MECHANISM OF RECOVERY IN PNEUMOCOCCAL PNEUMONIA , 1946, The Journal of experimental medicine.

[35]  C. G. Loosli.  THE HISTOGENESIS OF CELLS IN EXPERIMENTAL PNEUMONIA IN THE DOG , 1942, The Journal of experimental medicine.

[36]  J. Repine,et al.  Phagocytosis by human alveolar macrophages and neutrophils: qualitative differences in the opsonic requirements for uptake of Staphylococcus aureus and Streptococcus pneumoniae in vitro. , 1980, The American review of respiratory disease.

[37]  R. Rylander,et al.  Pulmonary cell response patterns after exposure to airborne bacteria. , 1975, Scandinavian journal of respiratory diseases.

[38]  R. Steigbigel,et al.  Phagocytic and Bactericidal Properties of Normal Human Monocytes , 1974 .

[39]  A. Böyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[40]  C. G. Loosli,et al.  ACUTE EXPERIMENTAL PNEUMOCOCCAL (TYPE I) PNEUMONIA IN THE MOUSE: THE MIGRATION OF LEUCOCYTES FROM THE PULMONARY CAPILLARIES INTO THE ALVEOLAR SPACES AS REVEALED BY THE ELECTRON MICROSCOPE. , 1962, Transactions of the American Clinical and Climatological Association.