Phenolic compounds in blackcurrant (Ribes nigrum L.) leaves relative to leaf position and harvest date.

Blackcurrant leaves are an essential source of phenolic compounds and this study investigated their variation relative to leaf positions and harvest date. The phenolic content varied between harvest dates, although leaf position on the shoot and interactions also played an important role. The contents of quercetin-malonyl-glucoside, kaempferol-malonyl-glucoside isomer and kaempferol-malonyl-glucoside were higher than that of the other identified phenolic compounds, whereas epigallocatechin was the lowest for all investigated leaf positions and harvest dates. The content of several of the compounds was highest in June, while quercetin-glucoside, kaempferol-glucoside and total phenols, increased towards the end of the season. Leaf position influenced the content of myricetin-malonyl-glucoside, myricetin-malonyl-glucoside isomer, quercetin-malonyl-glucoside and kaempferol-glucoside at the end of the season. Knowledge relating to the influence of ontogenetic and harvest time on the content of specific phenolic compounds might contribute in tailoring functional foods or pharmaceutical products using blackcurrant leaves as natural ingredients.

[1]  M. Matsuki Regulation of Plant Phenolic Synthesis: From Biochemistry to Ecology and Evolution , 1996 .

[2]  O. Laaksonen,et al.  Compositional differences of phenolic compounds between black currant (Ribes nigrum L.) cultivars and their response to latitude and weather conditions. , 2012, Journal of agricultural and food chemistry.

[3]  C. Ehrhardt,et al.  A Plant Extract of Ribes nigrum folium Possesses Anti-Influenza Virus Activity In Vitro and In Vivo by Preventing Virus Entry to Host Cells , 2013, PloS one.

[4]  R. Brennan,et al.  Improving Fruit Quality in Rubus and Ribes through Breeding , 2009 .

[5]  V. Ossipov,et al.  Variation of total phenolic content and individual low-molecular-weight phenolics in foliage of mountain birch trees (Betula pubescens ssp.tortuosa) , 1996, Journal of Chemical Ecology.

[6]  A. Wojdyło,et al.  Identification and characterization of low molecular weight polyphenols in berry leaf extracts by HPLC-DAD and LC-ESI/MS. , 2011, Journal of agricultural and food chemistry.

[7]  G. Rottinghaus,et al.  Occurrence of rutin and chlorogenic acid in elderberry leaf, flower, and stem in response to genotype, environment, and season , 2008 .

[8]  C. Tonelli,et al.  Diet and health. , 1962, Public health reports.

[9]  J. Koricheva,et al.  Seasonal changes in birch leaf chemistry: are there trade-offs between leaf growth and accumulation of phenolics? , 2002, Oecologia.

[10]  Laura R. Emery,et al.  Protein Phylogenetic Analysis of Ca2+/cation Antiporters and Insights into their Evolution in Plants , 2012, Front. Plant Sci..

[11]  K. Sutton,et al.  Understanding the health benefits of blackcurrants , 2002 .

[12]  V. Nour,et al.  Antioxidant capacity, phenolic compounds and minerals content of blackcurrant (Ribes nigrum L.) leaves as influenced by harvesting date and extraction method , 2014 .

[13]  R. Wrolstad,et al.  Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: vaccinium, rubus, and ribes. , 2002, Journal of agricultural and food chemistry.

[14]  S. Ou,et al.  Effects of latitude and weather conditions on contents of sugars, fruit acids, and ascorbic acid in black currant (Ribes nigrum L.) juice. , 2009, Journal of agricultural and food chemistry.

[15]  L. Jaakola,et al.  Phenolic Composition and Antioxidant Capacity of Bilberry (Vaccinium myrtillus) Leaves in Northern Europe Following Foliar Development and Along Environmental Gradients , 2010, Journal of Chemical Ecology.

[16]  A. Edreva The importance of non-photosynthetic pigments and cinnamic acid derivatives in photoprotection , 2005 .

[17]  D. Evers,et al.  Ascorbic acid, phenolic acid, flavonoid, and carotenoid profiles of selected extracts from Ribes nigrum. , 2011, Journal of agricultural and food chemistry.

[18]  J. Pincemail,et al.  Antioxidant capacity of black currant varies with organ, season, and cultivar. , 2006, Journal of agricultural and food chemistry.

[19]  J. Pincemail,et al.  Antioxidant and anti-inflammatory activities of Ribes nigrum extracts , 2012 .

[20]  L. Romero,et al.  Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. , 2001, Plant science : an international journal of experimental plant biology.

[21]  R. Gref,et al.  Plant-part specific and temporal variation in phenolic compounds of boreal bilberry (Vaccinium myrtillus) plants , 2003 .

[22]  E. Johansson,et al.  An optimized method for analysis of phenolic compounds in buds, leaves, and fruits of black currant ( Ribes nigrum L.). , 2012, Journal of agricultural and food chemistry.

[23]  Lack of UV radiation in Biosphere 2 — practical and theoretical effects on plants , 2000 .

[24]  P. Casati,et al.  Flavonoids: biosynthesis, biological functions, and biotechnological applications , 2012, Front. Plant Sci..

[25]  A. Törrönen,et al.  Flavonol content varies among black currant cultivars. , 2001, Journal of agricultural and food chemistry.

[26]  Sirpa Kärenlampi,et al.  Activation of flavonoid biosynthesis by solar radiation in bilberry (Vaccinium myrtillus L.) leaves , 2004, Planta.

[27]  S. Y. Wang,et al.  Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. , 2000, Journal of agricultural and food chemistry.

[28]  Jen-kun Lin,et al.  Composition of polyphenols in fresh tea leaves and associations of their oxygen-radical-absorbing capacity with antiproliferative actions in fibroblast cells , 1996 .

[29]  Baoru Yang,et al.  Flavonol glycosides and other phenolic compounds in buds and leaves of different varieties of black currant (Ribes nigrum L.) and changes during growing season. , 2014, Food chemistry.

[30]  G. Zdunić,et al.  Composition and antimicrobial activity of the essential oil of the leaves of black currant (Ribes nigrum L.) cultivar Čačanska crna , 2010 .

[31]  H. Dietrich,et al.  Effects of cultivar, yield, berry weight, temperature and ripening stage on bioactive compounds of black currants. , 2012 .

[32]  A. S. Darvesh,et al.  The health benefits of blackcurrants. , 2012, Food & function.

[33]  A. Szajdek,et al.  Bioactive Compounds and Health-Promoting Properties of Berry Fruits: A Review , 2008, Plant foods for human nutrition.

[34]  J. Koricheva,et al.  Low molecular mass phenolics in foliage of Betula pubescens Ehrh. in relation to aerial pollution , 1997 .

[35]  N. Garbacki,et al.  Proanthocyanidins, from Ribes nigrum leaves, reduce endothelial adhesion molecules ICAM-1 and VCAM-1 , 2005, Journal of Inflammation.

[36]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .

[37]  A. Kikas,et al.  Nutritional quality of berries and bioactive compounds in the leaves of black currant (Ribes nigrum L.) cultivars evaluated in Estonia , 2010 .

[38]  G. Costa,et al.  Composition of phenolic compounds in pear leaves as affected by genetics, ontogenesis and the environment , 2006 .

[39]  C. Declume Anti-inflammatory evaluation of a hydroalcoholic extract of black currant leaves (Ribes nigrum). , 1989, Journal of ethnopharmacology.

[40]  D. Stewart,et al.  A review on bioactive compounds in black currants (Ribes nigrum L.) and their potential health-promoting properties. , 2009 .