Analysis of the inhibition of sporulation of Bacillus subtilis caused by increasing the number of copies of the spo0F gene.

The Bacillus subtilis spo0F gene was cloned on a 6.3 kbp Bg/II fragment. The effect on sporulation of amplification of the spo0F region was examined. Sporulation was inhibited to less than 5% of that of the parental strain when as few as four copies of the spo0F region were present. Subclones, constructed in autonomous or integrative vectors, were used to demonstrate that the region responsible for the copy-number-dependent asporogeny corresponded closely with the spo0F structural gene. A possible mechanism for this effect is discussed.

[1]  J. Hoch,et al.  Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[2]  J. Hoch,et al.  Revised genetic linkage map of Bacillus subtilis , 1985, Microbiological reviews.

[3]  M. F. Bennett,et al.  Amplification of a major membrane-bound DNA sequence of Bacillus subtilis , 1985, Journal of bacteriology.

[4]  J. Hoch,et al.  Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein , 1985, Journal of bacteriology.

[5]  P. Piggot,et al.  Use of integrational plasmid vectors to demonstrate the polycistronic nature of a transcriptional unit (spoIIA) required for sporulation of Bacillus subtilis. , 1984, Journal of general microbiology.

[6]  M. Young.  Gene amplification in Bacillus subtilis. , 1984, Journal of general microbiology.

[7]  D. Hanahan.  Studies on transformation of Escherichia coli with plasmids. , 1983, Journal of molecular biology.

[8]  J. Hoch,et al.  Construction and properties of an integrable plasmid for Bacillus subtilis , 1983, Journal of bacteriology.

[9]  J. Messing.  [2] New M13 vectors for cloning , 1983 .

[10]  R. Losick,et al.  Mapping a cloned gene under sporulation control by inserttion of a drug resistance marker into the Bacillus subtilis chromosome , 1980, Journal of bacteriology.

[11]  A. Jeffreys,et al.  A physical map of the DNA regions flanking the rabbit β-globin gene , 1977, Cell.

[12]  E. Southern.  Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[13]  S. Falkow,et al.  General Method for the Isolation of Plasmid Deoxyribonucleic Acid , 1973, Journal of bacteriology.

[14]  J. Hoch,et al.  Chromosomal location of pleiotropic negative sporulation mutations in Bacillus subtilis. , 1973, Genetics.

[15]  J. Mandelstam,et al.  Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. , 1969, The Biochemical journal.