Relationship between conformationally sensitive probe binding sites on phosphorylase b.

Studies with three different paramagnetic probes indicate that the allosteric and active sites on Phosphorylase b are relatively close and the conformational change is confined to a small region.

[1]  F. Perrin.  Polarisation de la lumière de fluorescence. Vie moyenne des molécules dans l'etat excité , 1926 .

[2]  I. Solomon.  Relaxation Processes in a System of Two Spins , 1955 .

[3]  O. Jardetzky,et al.  Investigation of the Structure of Purines, Pyrimidines, Ribose Nucleosides and Nucleotides by Proton Magnetic Resonance. II1 , 1960 .

[4]  H. Mcconnell,et al.  Spin-labeled biomolecules. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Hedrick,et al.  An optical rotary dispersion study of glycogen phosphorylase. , 1966, Archives of biochemistry and biophysics.

[6]  D. Graves,et al.  Circular dichroism and optical rotatory dispersion of glycogen phosphorylase. , 1966, Biochemistry.

[7]  D. Koshland,et al.  The catalytic and regulatory properties of enzymes. , 1968, Annual review of biochemistry.

[8]  J. Taylor,et al.  Magnetic resonance studies of spin-labeled creatine kinase system and interaction of two paramagnetic probes. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Leigh.  ESR Rigid-Lattice Line Shape in a System of Two Interacting Spins , 1970 .

[10]  N. C. Price,et al.  The reactivity of SH groups with a fluorogenic reagent , 1970, FEBS letters.

[11]  M. Cohn.  Magnetic resonance studies of enzymesubstrate complexes with paramagnetic probes as illustrated by creatine kinase , 1970, Quarterly Reviews of Biophysics.

[12]  G K Radda,et al.  Enzyme and membrane conformation in biochemical control. , 1971, The Biochemical journal.

[13]  R. Dwek,et al.  Probes for the conformational transitions of phosphorylase b. Effect of ligands studied by proton relaxation enhancement, fluorescence and chemical reactivities. , 1971, European journal of biochemistry.