Inactivation Kinetics of Tomato Juice Lipoxygenase by Pulsed Electric Fields

ABSTRACT: Pulsed electric field (PEF) inactivation models for tomato juice lipoxygenase (LOX) were studied. Tomato juice was treated by PEF with the combinations of electric field strength (0, 10, 15, 20, 30, 35 kV/cm), PEF treatment time (20, 30, 50, 60, 70 μs), and PEF treatment temperature (10, 20, 30, 40, 50 °C). The first-order inactivation models, Hulsheger's model, Fermi's model, and the 2nd-order polynomial equation adequately described the LOX inactivation. Calculated D values were 161.0, 112.9, 101.0, and 74.8 μs at 15, 20, 30, and 35 kV/cm, respectively, at 30 °C. The activation energy for the inactivation of LOX by PEF was 35.7 kJ/mol. Applied electric field strength was the primary variable for the inactivation of LOX.

[1]  Gauri S. Mittal,et al.  Effects of high field electric pulses on the activity of selected enzymes , 1997 .

[2]  L. Cisneros-Zevallos,et al.  Thermal Inactivation Kinetics of Peroxidase and Lipoxygenase from Broccoli, Green Asparagus and Carrots , 2002 .

[3]  H. Hülsheger,et al.  Killing of bacteria with electric pulses of high field strength , 1981, Radiation and environmental biophysics.

[4]  T. Galliard,et al.  The enzymic breakdown of lipids to volatile and non‐volatile carbonyl fragments in disrupted tomato fruits , 1977 .

[5]  A. Yousef,et al.  Inactivation of Listeria monocytogenes in milk by pulsed electric field. , 1998, Journal of food protection.

[6]  C. Dunne,et al.  Inactivation of papain by pulsed electric fields in a continuous system , 1999 .

[7]  R. Rouseff,et al.  Fruit Flavors: Biogenesis, Characterization, and Authentication , 1995 .

[8]  Micha Peleg,et al.  A model of microbial survival after exposure to pulsed electric fields , 1995 .

[9]  Indrawati,et al.  Single, Combined, or Sequential Action of Pressure and Temperature on Lipoxygenase in Green Beans (Phaseolus vulgaris L.) : A Kinetic Inactivation Study , 1999, Biotechnology progress.

[10]  G. Evrendilek,et al.  Microbial safety and shelf-life of apple juice and cider processed by bench and pilot scale PEF systems , 2000 .

[11]  Gustavo V. Barbosa-Cánovas,et al.  Plasmin Inactivation with Pulsed Electric Fields , 1995 .

[12]  Q. Zhang,et al.  An integrated PEF pilot plant for continuous nonthermal pasteurization of fresh orange juice , 1998 .

[13]  H W Yeom,et al.  Effects of pulsed electric fields on the quality of orange juice and comparison with heat pasteurization. , 2000, Journal of agricultural and food chemistry.

[14]  G. Chism,et al.  Inactivation of Pectin Methyl Esterase in Orange Juice by Pulsed Electric Fields , 2002 .

[15]  Dietrich Knorr,et al.  DEVELOPMENTS OF NONTHERMAL PROCESSES FOR FOOD PRESERVATION , 1992 .

[16]  Argyrios Margaritis,et al.  Kinetics of sterilization of Lactobacillus brevis cells by the application of high voltage pulses , 1992, Biotechnology and bioengineering.

[17]  Q. Zhang,et al.  PULSED ELECTRIC FIELD INACTIVATION of MICROORGANISMS and PRESERVATION of QUALITY of CRANBERRY JUICE , 1999 .

[18]  Gustavo V. Barbosa-Cánovas,et al.  Inhibition of tomato (Licopersicon esculentum Mill.) pectin methylesterase by pulsed electric fields , 2000 .

[19]  Qinghua Zhang,et al.  Engineering aspects of pulsed electric field pasteurization , 1995 .