Reduced Porcine Islet Isolation Yield in the Presence of Hyperemic Islets

Abstract When studying histological characteristics of porcine pancreata in relation to islet isolation, a remarkably high number of hyperemic islets (HIs) was encountered. The abnormalities observed in these HIs ranged from a single dilated vessel to hemorrhages extending into the surrounding exocrine tissue. The aim of the present study was to compare pancreata with and without HI on islet isolation outcomes. This study involved a histological examination of 143 purebred (74 juvenile and 69 adult) and 47 crossbred (only juvenile) porcine pancreata. Islet isolation was performed in 48 purebred adult pigs and in 25 crossbred pigs. Tissue samples were stained with Aldehyde Fuchsine. The presence of HIs was scored semi-quantitatively (HI–, HI+). We observed HIs in 48% of the purebred and in 68% of the crossbred pigs. However, only 3.3±3.1% and 3.1±4.7% of all assessed islets was hyperemic in HI+ pancreata in purebred and crossbred pigs, respectively. In both groups, significantly higher endocrine cell mass was found in the HI+ pancreata (p<0.01). When the higher endocrine cell mass was taken into account, we found significantly lower yields in the HI+ pancreata in both purebred and crossbred pigs (p=0.03 in both groups). The presence of HIs occurs frequently in porcine donor-pancreata and is associated with reduced isolation outcomes.

[1]  P. J. Marang-van de Mheen,et al.  Presence of Hyperemic Islets in Human Donor-Pancreata Results in Reduced Islet Isolation Yield , 2011, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme.

[2]  H. Satoh,et al.  Age- and Sex-Related Differences in Spontaneous Hemorrhage and Fibrosis of the Pancreatic Islets in Sprague–Dawley Rats , 2007, Toxicologic pathology.

[3]  Camillo Ricordi,et al.  The unique cytoarchitecture of human pancreatic islets has implications for islet cell function , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Alvin C. Powers,et al.  Assessment of Human Pancreatic Islet Architecture and Composition by Laser Scanning Confocal Microscopy , 2005, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  P. Brunetti,et al.  Method for mass retrieval, morphologic, and functional characterization of adult porcine islets of Langerhans: a potential nonhuman pancreatic tissue resource for xenotransplantation in insulin-dependent diabetes mellitus. , 1995, Journal of investigative medicine : the official publication of the American Federation for Clinical Research.

[6]  R. van Schilfgaarde,et al.  Amount and Distribution of Collagen in Pancreatic Tissue of Different Species in the Perspective of Islet Isolation Procedures , 1995, Cell transplantation.

[7]  R. van Schilfgaarde,et al.  AMOUNT AND DISTRIBUTION OF COLLAGEN IN PANCREATIC TISSUE OF DIFFERENT SPECIES IN THE PERSPECTIVE OF ISLET ISOLATION PROCEDURES. , 1995, Cell transplantation.

[8]  H. Wacker,et al.  Histomorphological characteristics of the porcine pancreas as a basis for the isolation of islets of Langerhans , 1995 .

[9]  R. van Schilfgaarde,et al.  Distribution of collagens type I, type III and type V in the pancreas of rat, dog, pig and man , 1994, Cell and Tissue Research.

[10]  C. Ricordi,et al.  Selection of donors significantly improves pig islet isolation yield. , 1990, Hormone and metabolic research. Supplement series.

[11]  A. Siegfried,et al.  Morphology of the chronic toxicity of busulfan on the islets of Langerhans in the rat. , 1987, Hepato-Gastroenterology.

[12]  M. Hunt,et al.  Effects of Stunning Method and Time Interval from Stunning to Exsanguination on Blood Splashing in Pork1 , 1983 .

[13]  J. Findlay,et al.  Islet organ, blood glucose and glucose tolerance of lean and obese Mongolian gerbils , 1981, Cell and Tissue Research.

[14]  R. Coleman,et al.  Erthrocytes within pancreatic B-cells of corticosteroid-treated mice , 1978, Experientia.

[15]  C. Hulstaert,et al.  Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig, and man , 2004, Cell and Tissue Research.

[16]  S. White,et al.  An investigation into the distribution of different collagen types within adult and juvenile porcine pancreata , 1999, Journal of Molecular Medicine.

[17]  F. Brunicardi,et al.  Glucose-induced islet hyperemia is mediated by nitric oxide. , 1996, American journal of surgery.

[18]  D. Carr,et al.  The effect of different methods of electrical stunning of lambs on blood pressure and muscular activity. , 1986, Meat science.

[19]  T. Grandin.  Cardiac Arrest Stunning of Livestock and Poultry , 1986 .