The effect of colour light on production of zooids in 10 strains of the green chlorococcal alga Scenedesmus obliquus

The effect of lights of different spectral composition on the life cycle of 10 strains of Scenedesmus(Acutodesmus) obliquus (Turp.)Kutz. was studied. Subcultures from synchronous mother culture grown under standard conditions were withdrawn at 0, 4, 8, and 16 hours intervals and the dense cultures exposed in nitrogen free medium, temperature 15°C and various intensities of white, blue, red, or green light. Motile cells were observed only at cultures grown under blue or green light. Their number was independent on light intensity in tested span of irradiances (5, 25 and 50 μmol.m-2) that indicates signal role of blue and green light rather than a source of energy. The concentration of motile cells was dependent on developmental stage of cells shifted into the gametogenic conditions. It was proven that production of motile cells is strain specific because they were observed only in the two strains of ten tested.

[1]  V. Zachleder,et al.  The effect of cadmium ions on the cell cycle of the green flagellate Chlamydomonas noctigama , 2002 .

[2]  J. Lukavský,et al.  Behavior of chloroplast nucleoids in Scenedesmus obliquus during the vegetative cycle and under nitrogen starved conditions , 1998 .

[3]  J. Lukavský,et al.  Motile cells in outdoor mass culture of Scenedesmus obliquus II , 1998 .

[4]  F. R. Trainor,et al.  PRODUCING SCENEDESMUS OBLIQUUS GAMETES , 1995 .

[5]  E. Armbrust,et al.  A mating type-linked gene cluster expressed in Chlamydomonas zygotes participates in the uniparental inheritance of the chloroplast genome , 1993, Cell.

[6]  H. Weissig,et al.  Action Spectrum for the Light-Dependent Step in Gametic Differentiation of Chlamydomonas reinhardtii. , 1991, Plant physiology.

[7]  V. Zachleder,et al.  Relationship between gametic differentiation and the cell cycle in the green alga Chlamydomonas eugametos , 1991 .

[8]  J. R. Cain,et al.  REGULATION OF GAMETOGENESIS IN SCENEDESMUS OBLIQUUS (CHLOROPHYCEAE) 1 , 1976 .

[9]  C. A. Burg,et al.  Scenedesmus obliquus Sexuality , 1965, Science.

[10]  F. R. Trainor,et al.  Zoospores in Scenedesmus obliquus , 1963, Science.

[11]  Robert Staub Ernährungsphysiologisch-autökologische Untersuchungen an der planktischen Blaualge Oscillatoria rubescens DC. , 1961 .

[12]  E. Serrão,et al.  Blue- and green-light signals for gamete release in the brown alga, Silvetia compressa , 2003, Oecologia.

[13]  M. Haring,et al.  Gametic differentiation of Chlamydomonas , 1996 .

[14]  J. Lukavský Motile cells in Scenedesmus obliquus in outdoor mass culture , 1991 .

[15]  T. Kuroiwa,et al.  An Improved Method for the Demonstration of the in situ Chloroplast Nuclei in Higher Plants , 1980 .