Following the formation of supported lipid bilayers on mica: a study combining AFM, QCM-D, and ellipsometry.

Supported lipid bilayers (SLBs) are popular models of cell membranes with potential biotechnological applications and an understanding of the mechanisms of SLB formation is now emerging. Here we characterize, by combining atomic force microscopy, quartz crystal microbalance with dissipation monitoring, and ellipsometry, the formation of SLBs on mica from sonicated unilamellar vesicles using mixtures of zwitterionic, negatively and positively charged lipids. The results are compared with those we reported previously on silica. As on silica, electrostatic interactions were found to determine the pathway of lipid deposition. However, fundamental differences in the stability of surface-bound vesicles and the mobility of SLB patches were observed, and point out the determining role of the solid support in the SLB-formation process. The presence of calcium was found to have a much more pronounced influence on the lipid deposition process on mica than on silica. Our results indicate a specific calcium-mediated interaction between dioleoylphosphatidylserine molecules and mica. In addition, we show that the use of PLL-g-PEG modified tips considerably improves the AFM imaging of surface-bound vesicles and bilayer patches and evaluate the effects of the AFM tip on the apparent size and shape of these soft structures.

[1]  R. Richter,et al.  On the effect of the solid support on the interleaflet distribution of lipids in supported lipid bilayers. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[2]  A. Benda,et al.  Surface-dependent transitions during self-assembly of phospholipid membranes on mica, silica, and glass. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[3]  Alain Brisson,et al.  QCM-D on mica for parallel QCM-D-AFM studies. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[4]  Xuemei Liang,et al.  Probing small unilamellar EggPC vesicles on mica surface by atomic force microscopy. , 2004, Colloids and surfaces. B, Biointerfaces.

[5]  Olivier Felix,et al.  In situ investigations of the formation of mixed supported lipid bilayers close to the phase transition temperature , 2004 .

[6]  R. Richter,et al.  Pathways of lipid vesicle deposition on solid surfaces: a combined QCM-D and AFM study. , 2003, Biophysical journal.

[7]  G. Feigenson,et al.  Atomic force microscopy of nanometric liposome adsorption and nanoscopic membrane domain formation. , 2003, Ultramicroscopy.

[8]  Fredrik Höök,et al.  Characterization of DNA immobilization and subsequent hybridization on a 2D arrangement of streptavidin on a biotin-modified lipid bilayer supported on SiO2. , 2003, Analytical chemistry.

[9]  Fredrik Höök,et al.  Intact Vesicle Adsorption and Supported Biomembrane Formation from Vesicles in Solution: Influence of Surface Chemistry, Vesicle Size, Temperature, and Osmotic Pressure† , 2003 .

[10]  Lance C. Kam,et al.  Spatially Selective Manipulation of Supported Lipid Bilayers by Laminar Flow: Steps Toward Biomembrane Microfluidics† , 2003 .

[11]  R. Richter,et al.  Characterization of Lipid Bilayers and Protein Assemblies Supported on Rough Surfaces by Atomic Force Microscopy , 2003 .

[12]  S. Boxer,et al.  Early steps of supported bilayer formation probed by single vesicle fluorescence assays. , 2002, Biophysical journal.

[13]  B. Kasemo,et al.  Temperature dependence of formation of a supported phospholipid bilayer from vesicles on SiO2. , 2002, Physical review. E, Statistical, nonlinear, and soft matter physics.

[14]  B. Kasemo,et al.  Vesicle adsorption on SiO2 and TiO2: Dependence on vesicle size , 2002 .

[15]  A. Offenhäusser,et al.  Photopolymerization of diacetylene lipid bilayers and its application to the construction of micropatterned biomimetic membranes , 2002 .

[16]  Christian Le Grimellec,et al.  Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts , 2002, EMBO reports.

[17]  C. Yip,et al.  Aβ42-Peptide Assembly on Lipid Bilayers , 2002 .

[18]  E. Staples,et al.  On the consequences of surface treatment on the adsorption of nonionic surfactants at the hydrophilic silica-solution interface , 2002 .

[19]  W. Hermens,et al.  Muscovite (Mica) Allows the Characterisation of Supported Bilayers by Ellipsometry and Confocal Fluorescence Correlation Spectroscopy , 2002, Biological chemistry.

[20]  M. Textor,et al.  Biotin-Derivatized Poly(L-lysine)-g-poly(ethylene glycol): A Novel Polymeric Interface for Bioaffinity Sensing , 2002 .

[21]  I. Reviakine,et al.  Streptavidin 2D crystals on supported phospholipid bilayers: Toward constructing anchored phospholipid bilayers , 2001 .

[22]  C. Drummond,et al.  Surface roughness and surface force measurement: A comparison of electrostatic potentials derived from atomic force microscopy and electrophoretic mobility measurements , 2001 .

[23]  B. Kasemo,et al.  Comments on Rupture of Adsorbed Vesicles , 2001 .

[24]  A. Brisson,et al.  Two-dimensional crystallization of annexin A5 on phospholipid bilayers and monolayers : A solid-solid phase transition between crystal forms , 2001 .

[25]  Ka Yee C. Lee,et al.  Shape Evolution of Lipid Bilayer Patches Adsorbed on Mica: an Atomic Force Microscopy Study , 2001 .

[26]  T. Tjärnhage,et al.  From liposomes to supported, planar bilayer structures on hydrophilic and hydrophobic surfaces: an atomic force microscopy study. , 2000, Biophysical journal.

[27]  S. Boxer.  Molecular transport and organization in supported lipid membranes. , 2000, Current opinion in chemical biology.

[28]  N. Thompson,et al.  Formation and Characterization of Planar Phospholipid Bilayers Supported on TiO2 and SrTiO3 Single Crystals , 2000 .

[29]  V. Zhdanov,et al.  Formation of supported membranes from vesicles. , 2000, Physical review letters.

[30]  I. Reviakine,et al.  Formation of Supported Phospholipid Bilayers from Unilamellar Vesicles Investigated by Atomic Force Microscopy , 2000 .

[31]  V. Zhdanov,et al.  Simulation of adsorption kinetics of lipid vesicles , 2000 .

[32]  K. Furusawa,et al.  Liposome Adhesion on Mica Surface Studied by Atomic Force Microscopy , 1999 .

[33]  B. Kasemo,et al.  Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance. , 1998, Biophysical journal.

[34]  A. Yaroslavov,et al.  Integrity of mixed liposomes contacting a polycation depends on the negatively charged lipid content , 1998 .

[35]  Brisson,et al.  Growth of Protein 2-D Crystals on Supported Planar Lipid Bilayers Imaged in Situ by AFM. , 1998, Journal of structural biology.

[36]  Hayes,et al.  Direct Measurement of Colloidal Forces between Mica and Silica in Aqueous Electrolyte , 1997, Journal of colloid and interface science.

[37]  Udo Seifert,et al.  Configurations of fluid membranes and vesicles , 1997 .

[38]  S. Boxer,et al.  Architecture and function of membrane proteins in planar supported bilayers: a study with photosynthetic reaction centers. , 1996, Biochemistry.

[39]  Håkan Wennerström,et al.  Role of hydration and water structure in biological and colloidal interactions , 1996, Nature.

[40]  E. Sackmann,et al.  Supported Membranes: Scientific and Practical Applications , 1996, Science.

[41]  E. Sackmann,et al.  Phenomenology and kinetics of lipid bilayer spreading on hydrophilic surfaces , 1995 .

[42]  P. Nollert,et al.  Lipid vesicle adsorption versus formation of planar bilayers on solid surfaces. , 1995, Biophysical journal.

[43]  Fredrik Höök,et al.  Quartz crystal microbalance setup for frequency and Q‐factor measurements in gaseous and liquid environments , 1995 .

[44]  J. Chapel.  Electrolyte Species Dependent Hydration Forces between Silica Surfaces , 1994 .

[45]  B. Baibakov,et al.  A polycation causes migration of negatively charged phospholipids from the inner to outer leaflet of the liposomal membrane , 1994, FEBS letters.

[46]  Harland G. Tompkins,et al.  A User's Guide to Ellipsometry , 1992 .

[47]  H. Mcconnell,et al.  Line tension between liquid domains in lipid monolayers , 1992 .

[48]  U. Seifert,et al.  Adhesion of Vesicles and Membranes , 1991 .

[49]  E. Sackmann,et al.  Structure of an adsorbed dimyristoylphosphatidylcholine bilayer measured with specular reflection of neutrons. , 1991, Biophysical journal.

[50]  Seifert,et al.  Adhesion of vesicles. , 1990, Physical review. A, Atomic, molecular, and optical physics.

[51]  M. Bloom,et al.  Physical properties of single phospholipid bilayers adsorbed to micro glass beads. A new vesicular model system studied by 2H-nuclear magnetic resonance. , 1990, Biophysical journal.

[52]  R. Weis,et al.  Supported planar membranes in studies of cell-cell recognition in the immune system. , 1986, Biochimica et biophysica acta.

[53]  J. Kop,et al.  The role of intrinsic binding rate and transport rate in the adsorption of prothrombin, albumin, and fibrinogen to phospholipid bilayers , 1986 .

[54]  P. Marrack,et al.  Antigen presentation by supported planar membranes containing affinity-purified I-Ad. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[55]  H C Hemker,et al.  The adsorption of prothrombin to phosphatidylserine multilayers quantitated by ellipsometry. , 1983, The Journal of biological chemistry.

[56]  Richard M. Pashley,et al.  DLVO and hydration forces between mica surfaces in Li+, Na+, K+, and Cs+ electrolyte solutions: A correlation of double-layer and hydration forces with surface cation exchange properties , 1981 .

[57]  Richard M. Pashley,et al.  Hydration forces between mica surfaces in aqueous electrolyte solutions , 1981 .

[58]  G. Sauerbrey.  Verwendung von Schwingquarzen zur Wägung dünner Schichten und zur Mikrowägung , 1959 .

[59]  G. Sauerbrey,et al.  Use of quartz vibration for weighing thin films on a microbalance , 1959 .

[60]  G. Bolt.  Determination of the Charge Density of Silica Sols , 1957 .