WT1 and WT1‐AS genes are inactivated by promoter methylation in ovarian clear cell adenocarcinoma

Ovarian clear cell adenocarcinoma is associated with one of the poorest prognoses among human epithelial ovarian cancers. The authors hypothesized that Wilms tumor suppressor 1 gene (WT1) sense and antisense (WT1‐AS) expression and their promoter methylation status could characterize ovarian clear cell adenocarcinoma from ovarian serous adenocarcinoma.

[1]  D. Lubahn,et al.  Identification of DNA methylation markers for human breast carcinomas using the methylation-sensitive restriction fingerprinting technique. , 1997, Cancer research.

[2]  K. Malik,et al.  The molecular biology of Wilms' tumour , 2001, Expert Reviews in Molecular Medicine.

[3]  D. Lubahn,et al.  Hypermethylation of the Wilms' tumor suppressor gene CpG island in human breast carcinomas , 1999, Breast Cancer Research and Treatment.

[4]  M. McKay,et al.  Cancer of the ovary. , 1994, The New England journal of medicine.

[5]  D. Haber,et al.  Wilms tumor and the WT1 gene. , 2001, Experimental cell research.

[6]  I. Konishi,et al.  Immunohistochemical Detection of the Wilms' Tumor Gene (WT1) in Epithelial Ovarian Tumors , 2000, International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists.

[7]  C. B. Patel,et al.  Wilms' tumor suppressor gene (WT1) is expressed in primary breast tumors despite tumor-specific promoter methylation. , 2001, Cancer research.

[8]  B. Williams,et al.  Antisense transcripts and protein binding motifs within the Wilms tumour (WT1) locus. , 1994, Oncogene.

[9]  Kathleen R. Cho,et al.  Gene expression in ovarian cancer reflects both morphology and biological behavior, distinguishing clear cell from other poor-prognosis ovarian carcinomas. , 2002, Cancer research.

[10]  S. Cannistra Medical progress : cancer of the ovary , 1993 .

[11]  Osamu Ogawa,et al.  Cloning of novel Wilms tumor gene (WT1) cDNAs; evidence for antisense transcription of WT1. , 1994, Oncogene.

[12]  W. Hofmann,et al.  Characterization of the transcriptional regulatory region of the human WT1 gene. , 1993, Oncogene.

[13]  K. Malik,et al.  Identification of an antisense WT1 promoter in intron 1: implications for WT1 gene regulation. , 1995, Oncogene.

[14]  C. Walker,et al.  Identification of a tumor-specific methylation site in the Wilms tumor suppressor gene , 1998, Oncogene.

[15]  R. Dahiya,et al.  MULTIPLE PROMOTERS OF CATECHOL-O-METHYLTRANSFERASE GENE ARE SELECTIVELY INACTIVATED BY CPG HYPERMETHYLATION IN ENDOMETRIAL CANCER , 2003, International Journal of Gynecologic Cancer.

[16]  V. Kosma,et al.  Hypermethylation of the WT1 and calcitonin gene promoter regions at chromosome 11p in human colorectal cancer. , 1997, British Journal of Cancer.

[17]  Trevor Hastie,et al.  Gene expression patterns in ovarian carcinomas. , 2003, Molecular biology of the cell.

[18]  M. Gessler,et al.  Sequence of the WT1 upstream region including the Wit-1 gene. , 1993, Genomics.

[19]  James B. Mitchell,et al.  Distinct effects on gene expression of chemical and genetic manipulation of the cancer epigenome revealed by a multimodality approach. , 2004, Cancer cell.

[20]  A. Feinberg,et al.  Tissue, developmental, and tumor-specific expression of divergent transcripts in Wilms tumor. , 1990, Science.