Determination of dimethylamine and other low-molecular-mass amines using capillary electrophoresis with laser-induced fluorescence detection.

The potential of capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection for the separation and determination of dimethylamine (DMA) and other low-molecular-mass amines involving precolumn derivatization with fluorescein isothiocyanate isomer I (FITC) was investigated. Different variables that affect derivatization (pH, FITC concentration, reaction time and temperature) and separation (buffer concentration, addition of various organic modifiers, applied voltage and length of capillary) were studied. The linearity, reproducibility and reliability of the method were evaluated. The estimated instrumental detection limit for a 2-s pressure injection of the FITC-DMA derivative was 50 pg/ml (10(-9) M), using LIF detection with excitation and emission wavelengths of 488 nm and 520 nm, respectively. However, for practical reasons, a minimum of 5 ng/ml DMA should be subjected to the derivatization. The applicability of the described method to the extract of atmospheric aerosol samples was demonstrated.

[1]  P. Edman,et al.  A protein sequenator. , 1967, European journal of biochemistry.

[2]  P. Canal,et al.  Child cerebrospinal fluid analysis by capillary electrophoresis and laser-induced fluorescence detection. , 1995, Journal of Chromatography A.

[3]  M. Novotny,et al.  2-Methyl-3-oxo-4-phenyl-2,3-dihydrofuran-2-yl acetate: a fluorogenic reagent for detection and analysis of primary amines. , 1997, Analytical chemistry.

[4]  P. Rada,et al.  Capillary electrophoresis-laser-induced fluorescence detection of amphetamine in the brain. , 1996, Journal of chromatography. A.

[5]  Sam F. Y. Li,et al.  Separation of biogenic amines by micellar electrokinetic chromatography , 1996 .

[6]  C. Osterroht.  Extraction of dissolved fatty acids from sea water , 1993 .

[7]  P. Prognon,et al.  Luminarin 9: A new fluorescent reagent for the precolumn derivatizatioin of main volatile and non-volatile N-nitrosamines in RP-HPLC , 1994 .

[8]  N. Dovichi,et al.  Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence. , 1988, Science.

[9]  J. Pawliszyn,et al.  Determination of amines in air and water using derivatization combined with solid-phase microextraction , 1997 .

[10]  B. Renaud,et al.  Enhanced temporal resolution for the microdialysis monitoring of catecholamines and excitatory amino acids using capillary electrophoresis with laser-induced fluorescence detection. Analytical developments and in vitro validations. , 1996, Journal of chromatography. A.

[11]  N. Dovichi,et al.  Attomole amino acid determination by capillary zone electrophoresis with thermooptical absorbance detection. , 1989, Analytical chemistry.

[12]  V. S. Gaind,et al.  Quantification of Solid Sorbent-Sampled Airborne Aliphatic Polyamines on HPLC using a Common Calibration Standard -Application of the Concept of Isolation of a Selected n-System of a Derivative for Specific Detection , 1992 .

[13]  O. Busto,et al.  Improvement of a solid-phase extraction method for determining biogenic amines in wines , 1995 .

[14]  W. Grellner,et al.  Multiple cardiac rhabdomyoma with exclusively histological manifestation. , 1996, Forensic science international.

[15]  Maria Rosa Miracle,et al.  Determination of biogenic amines in wines by high-performance liquid chromatography with on-column fluorescence derivatization , 1997 .

[16]  F. Couderc,et al.  Determination of twenty eight biogenic amines and amino acids during wine aging by micellar electrokinetic chromatography and laser-induced fluorescence detection , 1997 .

[17]  M. Albin,et al.  Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques , 1991 .

[18]  C. Fu,et al.  High-performance liquid chromatography with post-column chemiluminescence detection for simultaneous determination of trace N-nitrosamines and corresponding secondary amines in groundwater , 1995 .

[19]  R. Wennrich,et al.  Sensitive Laser induced fluorescence detection of Polyamine-Fluoresceinisothiocyanate-derivatives after capillary zone electrophoretic separation , 1995 .

[20]  R. Lindahl,et al.  Determination of volatile amines in air by diffusive sampling, thiourea formation and high-performance liquid chromatography , 1993 .

[21]  P. Blanco,et al.  Determination of dimethylamine in groundwater by liquid chromatography and precolumn derivatization with 9-fluorenylmethylchloroformate , 1996 .

[22]  P. Leroy,et al.  CHIRAL DERIVATIZATION FOR SEPARATION OF RACEMIC AMINO AND THIOL DRUGS BY LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS , 1995 .

[23]  Zuliang Chen,et al.  Potentiometric detection of aliphatic amines by flow injection analysis and ion-interaction chromatography with a metallic copper electrode , 1997 .

[24]  H. Schulz,et al.  Determination of polyamines in biological materials by HPLC with 9-fluorenylmethyl chloroformate precolumn derivatization , 1995 .

[25]  P. Sandra,et al.  Practical and quantitative aspects in the analysis of FITC and DTAF amino acid derivatives by capillary electrophoresis and LIF detection , 1995 .

[26]  F. Sacher,et al.  Analysis of primary and secondary aliphatic amines in waste water and surface water by gas chromatography-mass spectrometry after derivatization with 2,4-dinitrofluorobenzene or benzenesulfonyl chloride , 1997 .

[27]  O. Busto,et al.  Determination of biogenic amines in wine after precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate , 1996 .

[28]  P. Jandera,et al.  Comparison of Various Sorbents for the Enrichment of Samples of Aliphatic Amines Using Solid-Phase Extraction Prior to the Determination by HPLC with Fluorimetric Detection , 1994 .

[29]  H. Swerdlow,et al.  Low-cost, high-sensitivity laser-induced fluorescence detection for DNA sequencing by capillary gel electrophoresis. , 1991, Journal of chromatography.

[30]  F. Couderc,et al.  Ball-lens laser-induced fluorescence detector as an easy-to-use highly sensitive detector for capillary electrophoresis application to the identification of biogenic amines in dairy products , 1995 .

[31]  W. C. Brumley,et al.  Determination of Aliphatic Amines in Water Using Derivatization with Fluorescein Isothiocyanate and Capillary Electrophoresis/Laser-Induced Fluorescence Detection , 1997 .

[32]  L. Monser,et al.  Liquid chromatographic determination of methylamines. Determination of trimethylamine in fish samples using a porous graphitic carbon stationary phase , 1996 .

[33]  H. Kawauchi,et al.  The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides. , 1984, Analytical biochemistry.

[34]  N. Guzman,et al.  Laser-induced fluorescence and fluorescence microscopy for capillary electrophoresis zone detection☆ , 1991 .

[35]  Yi-ming Liu,et al.  Determination of alcohols and amines, labelled with 4-(N,N-dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,3,1-benzoxadiazole, by liquid chromatography with conventional and laser-induced fluorescence detection , 1994 .

[36]  H. Kataoka,et al.  Derivatization reactions for the determination of amines by gas chromatography and their applications in environmental analysis. , 1996, Journal of chromatography. A.

[37]  J. F. Dlouhy,et al.  Automatic simultaneous determination of anions and cations in atmospheric aerosols by ion chromatography , 1993 .

[38]  H. Shintani,et al.  Handbook of Capillary Electrophoresis Applications , 1996 .

[39]  R. Kennedy,et al.  Quantitative in vivo monitoring of primary amines in rat caudate nucleus using microdialysis coupled by a flow-gated interface to capillary electrophoresis with laser-induced fluorescence detection. , 1996, Analytical chemistry.

[40]  M. I. Saleh,et al.  Separation of primary and secondary amines as their sulfonamide derivatives by reversed-phase high-performance liquid chromatography , 1997 .