Electrical properties of a light-addressable microelectrode chip with high electrode density for extracellular stimulation and recording of excitable cells.

A light-addressable microelectrode chip with 3600 TiN electrodes was fabricated. Amorphous silicon (a-Si:H) serves as a photo conductor. The electrodes on the chip are addressed by a laser spot and electrical properties of the system are determined. DC measurements show a dark to bright dynamic of 10(6)-10(7). The AC impedance dynamic @ 1 kHz/100 mV and thus the signal-to-noise-ratio is determined to 60. This value is quite sufficient for electrophysiological measurements. For the first time, recordings from cardiac myocytes are reported using the principle of light-addressing. Measurements were done with a standard laser scan microscope (Zeiss LSM 410).

[1]  Raymond P. Kesner,et al.  Electrical stimulation research techniques , 1981 .

[2]  R G Mark,et al.  Time delays in propagation of cardiac action potential. , 1990, The American journal of physiology.

[3]  J. Pine.  Recording action potentials from cultured neurons with extracellular microcircuit electrodes , 1980, Journal of Neuroscience Methods.

[4]  Gregory T. A. Kovacs,et al.  Planar electrode array systems for neural recording and impedance measurements , 1996, Proceedings of 18th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[5]  U. Egert,et al.  A thin film microelectrode array for monitoring extracellular neuronal activity in vitro. , 1994, Biosensors & bioelectronics.

[6]  Bernd K. Fleischmann,et al.  Action potential propagation failures in long-term recordings from embryonic stem cell-derived cardiomyocytes in tissue culture , 1999, Pflügers Archiv.

[7]  G. Gross.  Simultaneous Single Unit Recording in vitro with a Photoetched Laser Deinsulated Gold Multimicroelectrode Surface , 1979, IEEE Transactions on Biomedical Engineering.

[8]  Yu-Chong Tai,et al.  A cultured neuron probe , 1996, Proceedings of 18th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[9]  U. Egert,et al.  A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays. , 1998, Brain research. Brain research protocols.

[10]  G. H. Bauer,et al.  Raman-backscattering studies on amorphous hydrogenated silicon-germanium alloys , 1990 .