Applications and toxicity of graphene family nanomaterials and their composites.

Graphene has attracted much attention of scientific community due to its enormous potential in different fields, including medical sciences, agriculture, food safety, cancer research, and tissue engineering. The potential for widespread human exposure raises safety concerns about graphene and its derivatives, referred to as graphene family nanomaterials (GFNs). Due to their unique chemical and physical properties, graphene and its derivatives have found important places in their respective application fields, yet they are being found to have cytotoxic and genotoxic effects too. Since the discovery of graphene, a number of researches are being conducted to find out the toxic potential of GFNs to different cell and animal models, finding their suitability for being used in new and varied innovative fields. This paper presents a systematic review of the research done on GFNs and gives an insight into the mode and action of these nanosized moieties. The paper also emphasizes on the recent and up-to-date developments in research on GFNs and their nanocomposites for their toxic effects.

[1]  Omid Akhavan,et al.  Toxicity of graphene and graphene oxide nanowalls against bacteria. , 2010, ACS nano.

[2]  Hao Wang,et al.  Chlorotoxin-conjugated graphene oxide for targeted delivery of an anticancer drug , 2014, International journal of nanomedicine.

[3]  M. Costache,et al.  In vitro cytocompatibility evaluation of chitosan/graphene oxide 3D scaffold composites designed for bone tissue engineering. , 2014, Bio-medical materials and engineering.

[4]  R. Huo,et al.  Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring. , 2015, Materials science & engineering. C, Materials for biological applications.

[5]  Sai Bi,et al.  Magnetic graphene oxide-supported hemin as peroxidase probe for sensitive detection of thiols in extracts of cancer cells. , 2014, Biosensors & bioelectronics.

[6]  X. Cui,et al.  Electrically Controlled Drug Delivery from Graphene Oxide Nanocomposite Films , 2014, ACS nano.

[7]  Balaji Sitharaman,et al.  Synthesis, Characterization, In Vitro Phantom Imaging, and Cytotoxicity of A Novel Graphene-Based Multimodal Magnetic Resonance Imaging - X-Ray Computed Tomography Contrast Agent. , 2014, Journal of materials chemistry. B.

[8]  Intekhab Islam,et al.  Graphene: A Versatile Carbon-Based Material for Bone Tissue Engineering , 2015, Stem cells international.

[9]  Yi Cao,et al.  Hierarchical construction of a mechanically stable peptide-graphene oxide hybrid hydrogel for drug delivery and pulsatile triggered release in vivo. , 2015, Nanoscale.

[10]  L. Turng,et al.  The surface grafting of graphene oxide with poly(ethylene glycol) as a reinforcement for poly(lactic acid) nanocomposite scaffolds for potential tissue engineering applications. , 2016, Journal of the mechanical behavior of biomedical materials.

[11]  K. Hashem,et al.  Cerium oxide nanoparticles alleviate oxidative stress and decreases Nrf-2/HO-1 in D-GALN/LPS induced hepatotoxicity. , 2015, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie.

[12]  Xiaoyu Guo,et al.  Optimized core-shell Au@Ag nanoparticles for label-free Raman determination of trace Rhodamine B with cancer risk in food product. , 2015, Food chemistry.

[13]  Liang-Hong Guo,et al.  Single-walled carbon nanotubes and graphene oxides induce autophagosome accumulation and lysosome impairment in primarily cultured murine peritoneal macrophages. , 2013, Toxicology letters.

[14]  Hong Chen,et al.  A theranostic prodrug delivery system based on Pt(IV) conjugated nano-graphene oxide with synergistic effect to enhance the therapeutic efficacy of Pt drug. , 2015, Biomaterials.

[15]  C. Dimitrakopoulos,et al.  Wafer-Scale Graphene Integrated Circuit , 2011, Science.

[16]  Dirk Englund,et al.  High-Responsivity Graphene-Boron Nitride Photodetector and Autocorrelator in a Silicon Photonic Integrated Circuit. , 2015, Nano letters.

[17]  Ruo Yuan,et al.  Highly sensitive luminol electrochemiluminescence immunosensor based on ZnO nanoparticles and glucose oxidase decorated graphene for cancer biomarker detection. , 2012, Analytica chimica acta.

[18]  C. Fan,et al.  Uniform ultrasmall graphene oxide nanosheets with low cytotoxicity and high cellular uptake. , 2013, ACS applied materials & interfaces.

[19]  A. Nazarizadeh,et al.  Comparative Study of Antidiabetic Activity and Oxidative Stress Induced by Zinc Oxide Nanoparticles and Zinc Sulfate in Diabetic Rats , 2015, AAPS PharmSciTech.

[20]  M. Arshad,et al.  Zinc Oxide Nanoparticles for Revolutionizing Agriculture: Synthesis and Applications , 2014, TheScientificWorldJournal.

[21]  S. Haigh,et al.  Vertical field-effect transistor based on graphene-WS2 heterostructures for flexible and transparent electronics. , 2012, Nature nanotechnology.

[22]  Hilko van der Voet,et al.  Integrated probabilistic risk assessment for nanoparticles: the case of nanosilica in food , 2015, Journal of Nanoparticle Research.

[23]  K. Char,et al.  Delivery of bone morphogenetic protein-2 and substance P using graphene oxide for bone regeneration , 2014, International journal of nanomedicine.

[24]  Q. Qin,et al.  Fabrication and cyto-compatibility of Fe3O4/SiO2/graphene-CdTe QDs/CS nanocomposites for drug delivery. , 2014, Colloids and surfaces. B, Biointerfaces.

[25]  Li Du,et al.  Cytotoxicity of graphene oxide and graphene oxide loaded with doxorubicin on human multiple myeloma cells , 2014, International journal of nanomedicine.

[26]  Chi Bun Ching,et al.  Comparative protein profile of human hepatoma HepG2 cells treated with graphene and single-walled carbon nanotubes: an iTRAQ-coupled 2D LC-MS/MS proteome analysis. , 2011, Toxicology letters.

[27]  J. Dai,et al.  The in vitro and in vivo toxicity of graphene quantum dots. , 2014, Biomaterials.

[28]  Peng Huang,et al.  An aptamer-targeting photoresponsive drug delivery system using "off-on" graphene oxide wrapped mesoporous silica nanoparticles. , 2015, Nanoscale.

[29]  J. M. Navas,et al.  Internalization and cytotoxicity of graphene oxide and carboxyl graphene nanoplatelets in the human hepatocellular carcinoma cell line Hep G2 , 2013, Particle and Fibre Toxicology.

[30]  Jingyan Zhang,et al.  Insight into the Cellular Internalization and Cytotoxicity of Graphene Quantum Dots , 2013, Advanced healthcare materials.

[31]  Hung-Wei Yang,et al.  A reusable magnetic graphene oxide-modified biosensor for vascular endothelial growth factor detection in cancer diagnosis. , 2015, Biosensors & bioelectronics.

[32]  Y. Makita,et al.  Intracellular accumulation of indium ions released from nanoparticles induces oxidative stress, proinflammatory response and DNA damage. , 2016, Journal of biochemistry.

[33]  Yixiang Duan,et al.  A facile one-pot synthesis of starch functionalized graphene as nano-carrier for pH sensitive and starch-mediated drug delivery. , 2015, Colloids and surfaces. B, Biointerfaces.

[34]  Zhanfang Ma,et al.  Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites. , 2013, Biosensors & bioelectronics.

[35]  Hua He,et al.  Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery. , 2014, Colloids and surfaces. B, Biointerfaces.

[36]  Alberto Valdes Garcia,et al.  Graphene radio frequency receiver integrated circuit , 2014, Nature Communications.

[37]  Yongjun Li,et al.  Delivery of paclitaxel using PEGylated graphene oxide as a nanocarrier. , 2015, ACS applied materials & interfaces.

[38]  Azlin Mustapha,et al.  Toxicity of graphene oxide on intestinal bacteria and Caco-2 cells. , 2015, Journal of food protection.

[39]  Ruhong Zhou,et al.  Reduced Cytotoxicity of Graphene Nanosheets Mediated by Blood-Protein Coating. , 2015, ACS nano.

[40]  Mingsheng Chen,et al.  Biodegradable and pH-responsive nanoparticles designed for site-specific delivery in agriculture. , 2015, Biomacromolecules.

[41]  Yi Wang,et al.  Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria. , 2011, Biosensors & bioelectronics.

[42]  Yong Zhao,et al.  Electronic Detection of Bacteria Using Holey Reduced Graphene Oxide , 2014, ACS applied materials & interfaces.

[43]  I. Choi,et al.  Cytoprotective effects of graphene oxide for mammalian cells against internalization of exogenous materials. , 2013, Nanoscale.

[44]  L. Francis,et al.  Gravure Printing of Graphene for Large‐area Flexible Electronics , 2014, Advanced materials.

[45]  A. Striolo,et al.  Simulation insights for graphene-based water desalination membranes. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[46]  T. Seo,et al.  Quantitation of Oxidative Stress Gene Expression in Human Cell Lines Treated with Water-Dispersible MnO Nanoparticles. , 2015, Journal of nanoscience and nanotechnology.

[47]  Yachong Guo,et al.  Graphene Induces Formation of Pores That Kill Spherical and Rod-Shaped Bacteria. , 2015, ACS nano.

[48]  Dan Zhu,et al.  A graphene quantum dot-based FRET system for nuclear-targeted and real-time monitoring of drug delivery. , 2015, Nanoscale.

[49]  Rahul,et al.  Toxic Potential of Synthesized Graphene Zinc Oxide Nanocomposite in the Third Instar Larvae of Transgenic Drosophila melanogaster (hsp70-lacZ)Bg 9 , 2014, BioMed research international.

[50]  Kostas Kostarelos,et al.  Purified Graphene Oxide Dispersions Lack In Vitro Cytotoxicity and In Vivo Pathogenicity , 2013, Advanced healthcare materials.

[51]  S. S. Sinha,et al.  Bio-Conjugated CNT-Bridged 3D Porous Graphene Oxide Membrane for Highly Efficient Disinfection of Pathogenic Bacteria and Removal of Toxic Metals from Water. , 2015, ACS applied materials & interfaces.

[52]  Federica Chiellini,et al.  Ecotoxicity of pristine graphene to marine organisms. , 2014, Ecotoxicology and environmental safety.

[53]  N. Voelcker,et al.  Rhodamine-Functionalized Graphene Quantum Dots for Detection of Fe(3+) in Cancer Stem Cells. , 2015, ACS applied materials & interfaces.

[54]  N. Chatterjee,et al.  A systems toxicology approach to the surface functionality control of graphene-cell interactions. , 2014, Biomaterials.

[55]  Mei Jin,et al.  Cellular distribution and cytotoxicity of graphene quantum dots with different functional groups , 2014, Nanoscale Research Letters.

[56]  S. Faghihi,et al.  Synthesis and characterization of an octaarginine functionalized graphene oxide nano-carrier for gene delivery applications. , 2015, Physical chemistry chemical physics : PCCP.

[57]  M. Ahamed,et al.  Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells , 2015, Scientific Reports.

[58]  Seon Joo Park,et al.  High-performance flexible graphene aptasensor for mercury detection in mussels. , 2013, ACS nano.

[59]  Jun Liu,et al.  Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres. , 2010, Analytical chemistry.

[60]  Omid Akhavan,et al.  Size-dependent genotoxicity of graphene nanoplatelets in human stem cells. , 2012, Biomaterials.

[61]  F. Sharif,et al.  Graphene-oxide stabilization in electrolyte solutions using hydroxyethyl cellulose for drug delivery application. , 2015, International journal of pharmaceutics.

[62]  Yongsheng Zhou,et al.  Is graphene a promising nano-material for promoting surface modification of implants or scaffold materials in bone tissue engineering? , 2014, Tissue engineering. Part B, Reviews.

[63]  Yanli Zhao,et al.  Cancer cell detection and therapeutics using peroxidase-active nanohybrid of gold nanoparticle-loaded mesoporous silica-coated graphene. , 2015, ACS applied materials & interfaces.

[64]  S. Nanda,et al.  Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid , 2015, International journal of nanomedicine.

[65]  Woo Jin Hyun,et al.  High‐Resolution Patterning of Graphene by Screen Printing with a Silicon Stencil for Highly Flexible Printed Electronics , 2015, Advanced materials.

[66]  J. Dolatabadi,et al.  Nano graphene oxide: a novel carrier for oral delivery of flavonoids. , 2014, Colloids and surfaces. B, Biointerfaces.

[67]  W. Duan,et al.  Role of surface charge and oxidative stress in cytotoxicity and genotoxicity of graphene oxide towards human lung fibroblast cells , 2013, Journal of applied toxicology : JAT.

[68]  Sheng Dai,et al.  Water desalination using nanoporous single-layer graphene. , 2015, Nature nanotechnology.

[69]  G. Jiang,et al.  Cytotoxicity of quantum dots and graphene oxide to erythroid cells and macrophages , 2013, Nanoscale Research Letters.

[70]  J. Tour,et al.  Graphene oxide for effective radionuclide removal. , 2013, Physical chemistry chemical physics : PCCP.

[71]  Jing He,et al.  Electrochemical immunosensor with N-doped graphene-modified electrode for label-free detection of the breast cancer biomarker CA 15-3. , 2013, Biosensors & bioelectronics.

[72]  Zhen Gu,et al.  ATP-responsive DNA-graphene hybrid nanoaggregates for anticancer drug delivery. , 2015, Biomaterials.

[73]  K. Char,et al.  Delivery of a therapeutic protein for bone regeneration from a substrate coated with graphene oxide. , 2013, Small.

[74]  Li Mu,et al.  Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles. , 2015, Environmental science & technology.

[75]  E. Wang,et al.  Water desalination: Graphene cleans up water. , 2012, Nature nanotechnology.

[76]  D. García-Bernal,et al.  Effects of composite films of silk fibroin and graphene oxide on the proliferation, cell viability and mesenchymal phenotype of periodontal ligament stem cells , 2014, Journal of Materials Science: Materials in Medicine.

[77]  J. Grossman,et al.  Water desalination across nanoporous graphene. , 2012, Nano letters.

[78]  Shuai Wang,et al.  Hierarchical nanostructured noble metal/metal oxide/graphene-coated carbon fiber: in situ electrochemical synthesis and use as microelectrode for real-time molecular detection of cancer cells , 2015, Analytical and Bioanalytical Chemistry.

[79]  Prolonged Suppression of Neuropathic Pain by Sequential Delivery of Lidocaine and Thalidomide Drugs Using PEGylated Graphene Oxide. , 2015, Journal of pharmaceutical sciences.

[80]  J. Lan,et al.  Understanding the interactions of neptunium and plutonium ions with graphene oxide: scalar-relativistic DFT investigations. , 2014, The journal of physical chemistry. A.

[81]  Jinghua Yu,et al.  Magnetic graphene nanosheets based electrochemiluminescence immunoassay of cancer biomarker using CdTe quantum dots coated silica nanospheres as labels. , 2012, Talanta.

[82]  Jeong-Woo Choi,et al.  Cell chip to detect effects of graphene oxide nanopellet on human neural stem cell. , 2012, Journal of nanoscience and nanotechnology.

[83]  Tongsheng Chen,et al.  One-step reduction and PEGylation of graphene oxide for photothermally controlled drug delivery. , 2014, Biomaterials.

[84]  Shu-Pao Wu,et al.  Graphene Oxide Based Nanocarrier Combined with a pH-Sensitive Tracer: A Vehicle for Concurrent pH Sensing and pH-Responsive Oligonucleotide Delivery. , 2015, ACS applied materials & interfaces.

[85]  Agnes B Kane,et al.  Biological interactions of graphene-family nanomaterials: an interdisciplinary review. , 2012, Chemical research in toxicology.

[86]  Shun Mao,et al.  Ultrasonic-assisted self-assembly of monolayer graphene oxide for rapid detection of Escherichia coli bacteria. , 2013, Nanoscale.

[87]  Zhangqi Feng,et al.  Soft Graphene Nanofibers Designed for the Acceleration of Nerve Growth and Development , 2015, Advanced materials.

[88]  S. S. Sinha,et al.  Antimicrobial Peptide-Conjugated Graphene Oxide Membrane for Efficient Removal and Effective Killing of Multiple Drug Resistant Bacteria. , 2015, RSC advances.

[89]  Meng Li,et al.  Deciphering the underlying mechanisms of oxidation-state dependent cytotoxicity of graphene oxide on mammalian cells. , 2015, Toxicology letters.

[90]  O. Akhavan,et al.  Wrapping bacteria by graphene nanosheets for isolation from environment, reactivation by sonication, and inactivation by near-infrared irradiation. , 2011, The journal of physical chemistry. B.

[91]  K. Mallikarjuna,et al.  Bacopa monniera Stabilized Silver Nanoparticles Attenuates Oxidative Stress Induced by Aluminum in Albino Mice. , 2015, Journal of nanoscience and nanotechnology.

[92]  Yuezhou Wei,et al.  Understanding the bonding nature of uranyl ion and functionalized graphene: a theoretical study. , 2014, The journal of physical chemistry. A.

[93]  K. Chatterjee,et al.  Strontium eluting graphene hybrid nanoparticles augment osteogenesis in a 3D tissue scaffold. , 2015, Nanoscale.

[94]  Lei Zhang,et al.  Cytotoxicity effect of graphene oxide on human MDA-MB-231 cells , 2015, Toxicology mechanisms and methods.

[95]  Rahul,et al.  Evaluation of the Toxic Potential of Graphene Copper Nanocomposite (GCNC) in the Third Instar Larvae of Transgenic Drosophila melanogaster (hsp70-lacZ)Bg9 , 2013, PloS one.

[96]  Xingjiu Huang,et al.  Selective adsorption toward toxic metal ions results in selective response: electrochemical studies on a polypyrrole/reduced graphene oxide nanocomposite. , 2012, Chemical communications.

[97]  Jianguo Tian,et al.  Toward all-carbon electronics: fabrication of graphene-based flexible electronic circuits and memory cards using maskless laser direct writing. , 2010, ACS applied materials & interfaces.

[98]  T. Gao,et al.  Electrophoretically deposited graphene oxide and carbon nanotube composite for electrochemical capacitors , 2015, Nanotechnology.

[99]  I. Kymissis,et al.  Low-voltage organic electronics based on a gate-tunable injection barrier in vertical graphene-organic semiconductor heterostructures. , 2015, Nano letters.

[100]  Won Jong Kim,et al.  Photothermally controlled gene delivery by reduced graphene oxide-polyethylenimine nanocomposite. , 2014, Small.

[101]  Jason M Unrine,et al.  In Vivo Processing of Ceria Nanoparticles inside Liver: Impact on Free-Radical Scavenging Activity and Oxidative Stress. , 2014, ChemPlusChem.

[102]  Jong Seong Lee,et al.  Pulmonary Responses of Sprague-Dawley Rats in Single Inhalation Exposure to Graphene Oxide Nanomaterials , 2015, BioMed research international.

[103]  J. Robertson,et al.  Engineering high charge transfer n-doping of graphene electrodes and its application to organic electronics. , 2015, Nanoscale.

[104]  M. Dubey,et al.  Flexible integrated circuits and multifunctional electronics based on single atomic layers of MoS2 and graphene , 2015, Nanotechnology.

[105]  M. Mahmoudi,et al.  Hard corona composition and cellular toxicities of the graphene sheets. , 2013, Colloids and surfaces. B, Biointerfaces.

[106]  J. Riu,et al.  Graphene-based potentiometric biosensor for the immediate detection of living bacteria. , 2014, Biosensors & bioelectronics.

[107]  Baoan Chen,et al.  Layer-by-layer assembly of graphene, Au and poly(toluidine blue O) films sensor for evaluation of oxidative stress of tumor cells elicited by hydrogen peroxide. , 2013, Biosensors & bioelectronics.

[108]  Dong Yun Lee,et al.  In vivo biodistribution and toxicology of carboxylated graphene quantum dots. , 2013, ACS nano.

[109]  Chandre M. Willemse,et al.  Metallo-Graphene Nanocomposite Electrocatalytic Platform for the Determination of Toxic Metal Ions , 2011, Sensors.

[110]  A. Cao,et al.  Carbon Nanotube Network Embroidered Graphene Films for Monolithic All‐Carbon Electronics , 2015, Advanced materials.

[111]  Haifang Wang,et al.  Biological effect of food additive titanium dioxide nanoparticles on intestine: an in vitro study , 2015, Journal of applied toxicology : JAT.

[112]  Yang Song,et al.  Hyaluronic acid-decorated graphene oxide nanohybrids as nanocarriers for targeted and pH-responsive anticancer drug delivery. , 2014, ACS applied materials & interfaces.

[113]  Michael C. McAlpine,et al.  Graphene-based wireless bacteria detection on tooth enamel , 2012, Nature Communications.

[114]  Yang Xu,et al.  Single‐walled carbon nanotube and graphene nanodelivery of gambogic acid increases its cytotoxicity in breast and pancreatic cancer cells , 2014, Journal of applied toxicology : JAT.

[115]  Zhen Gu,et al.  Furin‐Mediated Sequential Delivery of Anticancer Cytokine and Small‐Molecule Drug Shuttled by Graphene , 2015, Advanced materials.

[116]  Liangzhu Feng,et al.  Graphene oxide-silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms. , 2013, ACS applied materials & interfaces.

[117]  Hua Sun,et al.  Toxicity of multi-walled carbon nanotubes, graphene oxide, and reduced graphene oxide to zebrafish embryos. , 2014, Biomedical and environmental sciences : BES.

[118]  Y. Niu,et al.  Detection of circulating tumor cells in prostate cancer based on carboxylated graphene oxide modified light addressable potentiometric sensor. , 2015, Biosensors & bioelectronics.

[119]  Jae Woong Han,et al.  Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa , 2012, International journal of nanomedicine.

[120]  Andre K. Geim,et al.  Electric Field Effect in Atomically Thin Carbon Films , 2004, Science.

[121]  Peng Xue,et al.  Paper-based microfluidic electrochemical immunodevice integrated with nanobioprobes onto graphene film for ultrasensitive multiplexed detection of cancer biomarkers. , 2013, Analytical chemistry.

[122]  Paula A A P Marques,et al.  Graphene oxide versus functionalized carbon nanotubes as a reinforcing agent in a PMMA/HA bone cement. , 2012, Nanoscale.

[123]  Homan Kang,et al.  Graphene oxide-encoded Ag nanoshells with single-particle detection sensitivity towards cancer cell imaging based on SERRS. , 2015, The Analyst.

[124]  Yang Xu,et al.  Cytotoxicity effects of graphene and single-wall carbon nanotubes in neural phaeochromocytoma-derived PC12 cells. , 2010, ACS nano.

[125]  Cheng Yang,et al.  Shape-Tailorable Graphene-Based Ultra-High-Rate Supercapacitor for Wearable Electronics. , 2015, ACS nano.

[126]  F. Sotgia,et al.  Graphene oxide selectively targets cancer stem cells, across multiple tumor types: Implications for non-toxic cancer treatment, via “differentiation-based nano-therapy” , 2015, Oncotarget.

[127]  Mingwei Wang,et al.  Graphene Oxide-Based Nanocarriers for Cancer Imaging and Drug Delivery. , 2015, Current pharmaceutical design.

[128]  Choon Nam Ong,et al.  Oxidative stress by inorganic nanoparticles. , 2016, Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology.

[129]  Jianping Gao,et al.  Porous graphene oxide/carboxymethyl cellulose monoliths, with high metal ion adsorption. , 2014, Carbohydrate polymers.

[130]  Jiali Zhang,et al.  DNA cleavage system of nanosized graphene oxide sheets and copper ions. , 2010, ACS nano.

[131]  Min Zhang,et al.  The effect of graphene oxide on conformation change, aggregation and cytotoxicity of HIV-1 regulatory protein (Vpr). , 2013, Biomaterials.

[132]  C. Zheng,et al.  Organic solvent-free cloud point extraction-like methodology using aggregation of graphene oxide. , 2014, Analytical chemistry.

[133]  Bin Du,et al.  Simultaneous electrochemical detection of cervical cancer markers using reduced graphene oxide-tetraethylene pentamine as electrode materials and distinguishable redox probes as labels. , 2014, Biosensors & bioelectronics.

[134]  P. Blom,et al.  Up-scaling graphene electronics by reproducible metal-graphene contacts. , 2015, ACS applied materials & interfaces.

[135]  Dongmei Wu,et al.  Graphene oxide/manganese ferrite nanohybrids for magnetic resonance imaging, photothermal therapy and drug delivery , 2016, Journal of biomaterials applications.

[136]  K. Sundar,et al.  Sodium selenite/selenium nanoparticles (SeNPs) protect cardiomyoblasts and zebrafish embryos against ethanol induced oxidative stress. , 2015, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[137]  Sang‐Woo Kim,et al.  Poly-4-vinylphenol and poly(melamine-co-formaldehyde)-based graphene passivation method for flexible, wearable and transparent electronics. , 2014, Nanoscale.

[138]  Nicolas H Voelcker,et al.  DNA melting and genotoxicity induced by silver nanoparticles and graphene. , 2015, Chemical research in toxicology.

[139]  Seunghwa Ryu,et al.  Ubiquitous Graphene Electronics on Scotch Tape , 2015, Scientific Reports.

[140]  Yanhui Li,et al.  Cytotoxicity of PEGylated graphene oxide on lymphoma cells. , 2014, Bio-medical materials and engineering.

[141]  Taihong Wang,et al.  Positive potential operation of a cathodic electrogenerated chemiluminescence immunosensor based on luminol and graphene for cancer biomarker detection. , 2011, Analytical chemistry.

[142]  Haifang Wang,et al.  Superior antibacterial activity of zinc oxide/graphene oxide composites originating from high zinc concentration localized around bacteria. , 2014, ACS applied materials & interfaces.

[143]  Yang Xu,et al.  Toxicity and efficacy of carbon nanotubes and graphene: the utility of carbon-based nanoparticles in nanomedicine , 2014, Drug metabolism reviews.

[144]  Z. Liao,et al.  Vertically architectured stack of multiple graphene field-effect transistors for flexible electronics. , 2015, Small.

[145]  M. Vila,et al.  Optimized graphene oxide foam with enhanced performance and high selectivity for mercury removal from water. , 2016, Journal of hazardous materials.

[146]  A. Tuantranont,et al.  Cytotoxicity assessment of MDA-MB-231 breast cancer cells on screen-printed graphene-carbon paste substrate. , 2014, Colloids and surfaces. B, Biointerfaces.

[147]  ZhiYong Qian,et al.  Biodegradable CSMA/PECA/Graphene Porous Hybrid Scaffold for Cartilage Tissue Engineering , 2015, Scientific Reports.

[148]  A. Lampen,et al.  Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells , 2015, Biological chemistry.

[149]  Gang Cheng,et al.  Cyclic RGD-modified chitosan/graphene oxide polymers for drug delivery and cellular imaging. , 2014, Colloids and surfaces. B, Biointerfaces.

[150]  Balaji Sitharaman,et al.  Cell specific cytotoxicity and uptake of graphene nanoribbons. , 2013, Biomaterials.

[151]  D. Gournis,et al.  Graphene oxide stabilized by PLA-PEG copolymers for the controlled delivery of paclitaxel. , 2015, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[152]  M. Plascencia-Jatomea,et al.  Controlled release matrices and micro/nanoparticles of chitosan with antimicrobial potential: development of new strategies for microbial control in agriculture. , 2013, Journal of the science of food and agriculture.

[153]  F. Ahmed,et al.  Investigation of acute effects of graphene oxide on wastewater microbial community: a case study. , 2013, Journal of hazardous materials.

[154]  Miguel Valcárcel,et al.  Graphene quantum dots in analytical science , 2015 .

[155]  Sihui Zhan,et al.  Highly efficient removal of pathogenic bacteria with magnetic graphene composite. , 2015, ACS applied materials & interfaces.

[156]  Q. Wei,et al.  Electrochemical immunosensor for detecting typical bladder cancer biomarker based on reduced graphene oxide-tetraethylene pentamine and trimetallic AuPdPt nanoparticles. , 2015, Talanta.

[157]  Miguel Valcárcel,et al.  Sulfonated nanocellulose for the efficient dispersive micro solid-phase extraction and determination of silver nanoparticles in food products. , 2016, Journal of chromatography. A.

[158]  Wenhao Dai,et al.  Multifunctional Poly(L-lactide)-Polyethylene Glycol-Grafted Graphene Quantum Dots for Intracellular MicroRNA Imaging and Combined Specific-Gene-Targeting Agents Delivery for Improved Therapeutics. , 2015, ACS applied materials & interfaces.

[159]  Christy L Haynes,et al.  Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. , 2011, ACS applied materials & interfaces.

[160]  M. Kamal,et al.  Role of Graphene Nano-Composites in Cancer Therapy: Theranostic Applications, Metabolic Fate and Toxicity Issues. , 2014, Current drug metabolism.

[161]  David Cohen-Tanugi,et al.  Water permeability of nanoporous graphene at realistic pressures for reverse osmosis desalination. , 2014, The Journal of chemical physics.

[162]  Sandhya Mishra,et al.  Biosynthesized silver nanoparticles as a nanoweapon against phytopathogens: exploring their scope and potential in agriculture , 2015, Applied Microbiology and Biotechnology.

[163]  J. White,et al.  Graphene in the aquatic environment: adsorption, dispersion, toxicity and transformation. , 2014, Environmental science & technology.

[164]  David Cohen-Tanugi,et al.  Mechanical strength of nanoporous graphene as a desalination membrane. , 2014, Nano letters.

[165]  Lin Zhao,et al.  Protein corona mitigates the cytotoxicity of graphene oxide by reducing its physical interaction with cell membrane. , 2015, Nanoscale.

[166]  Mostafa Azimzadeh,et al.  An electrochemical nanobiosensor for plasma miRNA-155, based on graphene oxide and gold nanorod, for early detection of breast cancer. , 2016, Biosensors & bioelectronics.

[167]  Balaji Sitharaman,et al.  Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme. , 2015, Nanomedicine : nanotechnology, biology, and medicine.

[168]  Ping Wang,et al.  Multi-nanomaterial electrochemical biosensor based on label-free graphene for detecting cancer biomarkers. , 2014, Biosensors & bioelectronics.

[169]  J. Li,et al.  Toxicity of Graphene Quantum Dots in Zebrafish Embryo. , 2015, Biomedical and environmental sciences : BES.

[170]  M. D. Ferreira,et al.  Polyethylene Films Containing Silver Nanoparticles for Applications in Food Packaging: Characterization of Physico-Chemical and Anti-Microbial Properties. , 2015, Journal of nanoscience and nanotechnology.

[171]  Wencheng Song,et al.  Plasma-induced grafting of polyacrylamide on graphene oxide nanosheets for simultaneous removal of radionuclides. , 2015, Physical chemistry chemical physics : PCCP.

[172]  S. Santucci,et al.  Flake size-dependent cyto and genotoxic evaluation of graphene oxide on in vitro A549, CaCo2 and vero cell lines. , 2014, Journal of biological regulators and homeostatic agents.

[173]  S. Yun,et al.  Mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes , 2015, Bioprocess and Biosystems Engineering.

[174]  A. Gao,et al.  Cross talk between poly(ADP-ribose) polymerase 1 methylation and oxidative stress involved in the toxic effect of anatase titanium dioxide nanoparticles , 2015, International journal of nanomedicine.

[175]  Liyuan Ma,et al.  Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide , 2014, Nanomaterials.

[176]  S. Abbasi,et al.  Synthesis and application of ion-imprinted polymer nanoparticles for the extraction and preconcentration of mercury in water and food samples employing cold vapor atomic absorption spectrometry , 2015, Environmental Monitoring and Assessment.

[177]  Bo Zhang,et al.  Towards intrinsic graphene biosensor: A label-free, suspended single crystalline graphene sensor for multiplex lung cancer tumor markers detection. , 2015, Biosensors & bioelectronics.

[178]  Kai Yang,et al.  Surface coating-dependent cytotoxicity and degradation of graphene derivatives: towards the design of non-toxic, degradable nano-graphene. , 2014, Small.

[179]  Deepthy Menon,et al.  Differential nano-bio interactions and toxicity effects of pristine versus functionalized graphene. , 2011, Nanoscale.

[180]  Bobby G. Sumpter,et al.  Tunable water desalination across graphene oxide framework membranes. , 2014, Physical chemistry chemical physics : PCCP.

[181]  Ning Yu,et al.  Reduced aggregation and cytotoxicity of amyloid peptides by graphene oxide/gold nanocomposites prepared by pulsed laser ablation in water. , 2014, Small.

[182]  J. Lewis,et al.  Rapid and Versatile Photonic Annealing of Graphene Inks for Flexible Printed Electronics , 2015, Advanced materials.

[183]  Zoraida P. Aguilar,et al.  Detection of the ovarian cancer biomarker CA-125 using chemiluminescence resonance energy transfer to graphene quantum dots. , 2014, Chemical communications.

[184]  W. N. Chen,et al.  Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human hepatoma HepG2 cells: an iTRAQ-coupled 2D LC-MS/MS proteome analysis. , 2012, Toxicological sciences : an official journal of the Society of Toxicology.

[185]  Jinglin Zhang,et al.  Sodium 1-naphthalenesulfonate-functionalized reduced graphene oxide stabilizes silver nanoparticles with lower cytotoxicity and long-term antibacterial activity. , 2012, Chemistry, an Asian journal.

[186]  Jaesung Park,et al.  Graphene‐Encapsulated Nanoparticle‐Based Biosensor for the Selective Detection of Cancer Biomarkers , 2011, Advanced materials.

[187]  J. Lackner,et al.  Thrombogenicity and biocompatibility studies of reduced graphene oxide modified acellular pulmonary valve tissue. , 2015, Materials science & engineering. C, Materials for biological applications.

[188]  S. Yao,et al.  Polyamidoamine dendrimer and oleic acid-functionalized graphene as biocompatible and efficient gene delivery vectors. , 2014, ACS applied materials & interfaces.

[189]  Waqar Ahmed,et al.  PEGylated graphene oxide for tumor-targeted delivery of paclitaxel. , 2015, Nanomedicine.

[190]  Jing Kong,et al.  Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress. , 2011, ACS nano.

[191]  D. Rodrigues,et al.  Toxicity of a polymer-graphene oxide composite against bacterial planktonic cells, biofilms, and mammalian cells. , 2012, Nanoscale.

[192]  L. Amelse,et al.  Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: potential for bone tissue engineering , 2015, Journal of applied toxicology : JAT.

[193]  Y. Agrawal,et al.  Curcumin loaded self assembled lipid-biopolymer nanoparticles for functional food applications , 2015, Journal of Food Science and Technology.

[194]  G. Neelgund,et al.  Hydroxyapatite grafted carbon nanotubes and graphene nanosheets: promising bone implant materials. , 2014, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.