Label-free electrochemical aptasensor constructed by layer-by-layer technology for sensitive and selective detection of cancer cells.

Here, a cytosensor was constructed with ferrocene-appended poly(allylamine hydrochloride) (Fc-PAH) functionalized graphene (Fc-PAH-G), poly(sodium-p-styrenesulfonate) (PSS) and aptamer (AS1411) by layer-by-layer assembly technology. The hybrid nanocomposite Fc-PAH-G not only brings probes on the electrode and also promotes electron transfer between the probes and the substrate electrode. Meanwhile, LBL technology provides more effective probes to enhance amplified signal for improving the sensitivity of the detection. While AS1411 forming G-quardruplex structure and binding cancer cells, the current response of the sensing electrode decreased due to the insulating properties of cellular membrane. Differential pulse voltammetry (DPV) was performed to investigate the electrochemical detection of HeLa cells attributing to its sensitivity of the current signal change. The as-prepared aptasensor showed a high sensitivity and good stability, a widely detection range from 10 to 10(6) cells/mL with a detection limit as low as 10 cells/mL for the detection of cancer cells.

[1]  Jose H. Hodak,et al.  Layer-by-Layer Self-Assembly of Glucose Oxidase with a Poly(allylamine)ferrocene Redox Mediator , 1997 .

[2]  Jing Li,et al.  High-sensitivity determination of lead and cadmium based on the Nafion-graphene composite film. , 2009, Analytica chimica acta.

[3]  K. Loh,et al.  High-throughput synthesis of graphene by intercalation-exfoliation of graphite oxide and study of ionic screening in graphene transistor. , 2009, ACS nano.

[4]  Yuehe Lin,et al.  Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells. , 2010, Journal of the American Chemical Society.

[5]  Jin Wang,et al.  A simple and rapid electrochemical strategy for non-invasive, sensitive and specific detection of cancerous cell. , 2013, Talanta.

[6]  Zhenxin Wang,et al.  Discrimination and detection of bacteria with a label-free impedimetric biosensor based on self-assembled lectin monolayer , 2011 .

[7]  R. Mahajan,et al.  A regenerative electrochemical sensor based on oligonucleotide for the selective determination of mercury(II). , 2009, The Analyst.

[8]  Min Su,et al.  Synthesis and characterization of graphene nanosheets attached to spiky MnO2 nanospheres and its application in ultrasensitive immunoassay , 2013 .

[9]  Xiaoru Zhang,et al.  A new photoelectrochemical aptasensor for the detection of thrombin based on functionalized graphene and CdSe nanoparticles multilayers. , 2011, Chemical communications.

[10]  Yanbin Li,et al.  Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. , 2004, Analytical chemistry.

[11]  E. Wang,et al.  An electrochemical aptasensor for chiral peptide detection using layer-by-layer assembly of polyelectrolyte-methylene blue/polyelectrolyte-graphene multilayer. , 2012, Analytica chimica acta.

[12]  X. Qu,et al.  A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells. , 2011, Biomaterials.

[13]  Weihong Tan,et al.  Molecular Recognition of Small‐Cell Lung Cancer Cells Using Aptamers , 2008, ChemMedChem.

[14]  Yinan Qin,et al.  Highly sensitive and selective detection of cancer cell with a label-free electrochemical cytosensor. , 2013, Biosensors & bioelectronics.

[15]  Caifeng Ding,et al.  Electrochemical and electrochemiluminescence determination of cancer cells based on aptamers and magnetic beads. , 2010, Chemistry.

[16]  Her-Hsiung Huang.  In situ surface electrochemical characterizations of Ti and Ti-6Al-4V alloy cultured with osteoblast-like cells. , 2004, Biochemical and biophysical research communications.

[17]  X. Qu,et al.  Cancer biomarker detection: recent achievements and challenges. , 2015, Chemical Society reviews.

[18]  Shana O Kelley,et al.  Rapid and specific electrochemical detection of prostate cancer cells using an aperture sensor array. , 2013, Lab on a chip.

[19]  Minghui Yang,et al.  Electroanalytical and surface plasmon resonance sensors for detection of breast cancer and Alzheimer's disease biomarkers in cells and body fluids. , 2014, The Analyst.

[20]  Peiping Zhu,et al.  Evaluation of x-ray diffraction enhanced imaging in the diagnosis of breast cancer , 2007, Physics in medicine and biology.

[21]  Feng Yan,et al.  Biomedical and clinical applications of immunoassays and immunosensors for tumor markers , 2007 .

[22]  Ashutosh Tiwari,et al.  Ultrasensitive detection of human liver hepatocellular carcinoma cells using a label-free aptasensor. , 2014, Analytical chemistry.

[23]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[24]  Kevin Brindle,et al.  New approaches for imaging tumour responses to treatment , 2008, Nature Reviews Cancer.

[25]  Hua Zhang,et al.  Graphene-based electrochemical sensors. , 2013, Small.

[26]  Bingling Li,et al.  "Fitting" makes "sensing" simple: label-free detection strategies based on nucleic acid aptamers. , 2013, Accounts of chemical research.

[27]  Chuangui Wang,et al.  Lectin-based biosensor strategy for electrochemical assay of glycan expression on living cancer cells. , 2010, Analytical chemistry.

[28]  Jing‐Juan Xu,et al.  Cytosensing and evaluation of cell surface glycoprotein based on a biocompatible poly(diallydimethylammonium) doped poly(dimethylsiloxane) film. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[29]  X. Qu,et al.  Ultrasensitive Telomerase Activity Detection in Circulating Tumor Cells Based on DNA Metallization and Sharp Solid‐State Electrochemical Techniques , 2014 .