Why Superoxide Imposes an Aromatic Amino Acid Auxotrophy onEscherichia coli

The lack of superoxide dismutase and the consequent elevation of [O2 −] imposes, onEscherichia coli, auxotrophies for branched chain, sulfur-containing, and aromatic amino acids. The former two classes of auxotrophies have already been explained, whereas the third is explained herein. Thus O2 − is shown to interfere with the production of erythrose-4-phosphate, which is essential for the first step of the aromatic biosynthetic pathway. It does so by oxidizing the 1,2-dihydroxyethyl thiamine pyrophosphate intermediate of transketolase and inactivating this enzyme.

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