Lipopolysaccharide induces retroviral antigen expression in 129/J mouse lymphocytes: evidence for assembly of a defective viral particle

In contrast to those of many other mouse strains, spleen cell cultures of 129/J mice do not release reverse transcriptase activity into the supernatant upon stimulation with bacterial lipopolysaccharide. We report here that lipopolysaccharide induced the expression of intracellular viral proteins in 129/J spleen cells. Furthermore, we found that stimulated spleen cells released retroviral particles. We conclude that 129/J mice are inducible with lipopolysaccharide but that the virus produced is a defective particle deficient in reverse transcriptase activity.

[1]  R. Monckton,et al.  Foetal calf serum acts as an inducer of endogenous C-type virus in mouse lymphoid cells. , 1980, The Journal of general virology.

[2]  S. Aaronson,et al.  Immunological relationships of an endogenous guinea pig retrovirus with prototype mammalian type B and type D retroviruses , 1980, Journal of virology.

[3]  J. Jongstra,et al.  Normal B-cell activation involves endogenous retroviral antigen expression: implications for leukemogenesis. , 1980, Cold Spring Harbor symposia on quantitative biology.

[4]  R. Weinberg,et al.  Identification of DNA fragments carrying ecotropic proviruses of AKR mice. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Levy,et al.  Genetics of xenotropic virus expression in mice. I. Evidence for a single locus regulating spontaneous production of infectious virus in crosses involving NZB/B1NJ and 129/J strains of mice , 1979, Journal of virology.

[6]  A. Coutinho,et al.  Receptor interactions on the membrane of resting and activated B cells , 1978, Nature.

[7]  C. Kozak,et al.  Genetic mapping of xenotropic leukemia virus-inducing loci in two mouse strains. , 1978, Science.

[8]  C. Moroni,et al.  Mitogen induction of murine C-type viruses. IV. Effects of lipoprotein E. coli, pokeweed mitogen and dextran sulphate. , 1978, The Journal of general virology.

[9]  J. Ihle,et al.  Expression of AKR murine leukemia virus gp71-like and BALB(X) gp-71- like antigens in normal mouse tissues in the absence of overt virus expression , 1977, The Journal of experimental medicine.

[10]  C. Moroni,et al.  Mitogen induction of murine C-type viruses. III. Effect of culture conditions, age, and genotype. , 1977, Virology.

[11]  A. Coutinho,et al.  Frequencies of mitogen-reactive B cells in the mouse. I. Distribution in different lymphoid organs from different inbred strains of mice at different ages , 1977, The Journal of experimental medicine.

[12]  A. Coutinho,et al.  Frequencies of mitogen-reactive B cells in the mouse. II. Frequencies of B cells producing antibodies which lyse sheep or horse erythrocytes, and trinitrophenylated or nitrodophenylated sheep erythrocytes , 1977, The Journal of experimental medicine.

[13]  J. Stephenson,et al.  Genetic factors infuencing mouse type-C RNA virus induction by naturally occurring B cell mitogens. , 1977, Journal of immunology.

[14]  H. Fan,et al.  Monospecific immunoprecipitation of murine leukemia virus polyribosomes: Identification of p30 protein-specific menssenger RNA , 1976, Cell.

[15]  R. Schwartz,et al.  Genetics of expression of xenotropic virus and autoimmunity in NZB mice , 1976, Nature.

[16]  C. Moroni,et al.  Mitogen induction of murine C-type viruses. II. Effect of B-lymphocyte mitogens. , 1976, Virology.

[17]  C. Moroni,et al.  Mitogen induction of murine C-type viruses. I. Analysis of lymphoid cell subpopulations. , 1976, Journal of immunology.

[18]  J. Stephenson,et al.  Induction of mouse type-C RNA virus by lipopolysaccharide. , 1975, Journal of immunology.

[19]  D. Lowy,et al.  Definitive evidence that the murine C-type virus inducing locus Akv-1 is viral genetic material. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[20]  M. Robert-Guroff,et al.  Induction of endogenous murine C-type virus in spleen cell cultures treated with mitogens and 5-bromo-2'-deoxyuridine. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[21]  E. Vitetta,et al.  Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus , 1975, The Journal of experimental medicine.

[22]  V. Moennig,et al.  Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. , 1974, Virology.

[23]  J. Stephenson,et al.  Demonstration of a genetic factor influencing spontaneous release of a xenotropic virus of mouse cells. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[24]  J. August,et al.  Host control of endogenous murine leukemia virus gene expression: concentrations of viral proteins in high and low leukemia mouse strains. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[25]  D. Lowy,et al.  AKR murine leukemia virus genome: frequency of sequences in DNA of high-, low-, and non-virus-yielding mouse strains. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[26]  J. Hartley,et al.  STUDIES OF GENETIC TRANSMISSION OF MURINE LEUKEMIA VIRUS BY AKR MICE , 1972, The Journal of experimental medicine.

[27]  R. Nowinski,et al.  Detection of avian and mammalian oncogenic RNA viruses (oncornaviruses) by immunofluorescence. , 1972, Cancer Research.

[28]  L. Old,et al.  THE GIX SYSTEM , 1971, The Journal of experimental medicine.

[29]  A. Cunningham,et al.  Further improvements in the plaque technique for detecting single antibody-forming cells. , 1968, Immunology.