PURPOSE: To compare the effectiveness of pulsed (impedance control mode) and non-pulsed (manual control mode) radiofrequency ablation (RFA) by using an internal cooled-clustered electrode. MATERIALS AND METHODS: Ex vivo RF ablations (n = 93) were performed with a 200 W RF generator (Model-Cooled Tip, Radionics, USA) in 10 bovine livers, using the impedance and manual control mode. In the impedance control mode, the generator automatically adjusted the applied RF power to the tissue impedance measured during RF ablation. In the manual control mode, the application of the RF power was constant. Both applications were investigated in a capacity range between 5 and 200 W. The duration for each RF ablation was between 2 and 60 minutes. After RF ablation, the short axis diameter of the necrosis was determined macroscopically and the peripheral zone of the necrosis histologically. RESULTS: The impedance control mode produced lesions with short axis diameters of 5.3 +/- 0.3 cm at a power of 200 W after 60 minutes of RF ablation and the manual control mode lesions with short axis diameters of 4.1 +/- 0.4 cm at a power of 70 W after 8 +/- 2 minutes of RF ablation. The impedance control mode increased significantly the time of RF ablation with higher power and the size of necrosis (p < 0.01). CONCLUSION: In comparison to non-pulsed RF ablation, pulsed RF ablation with internal cooled-clustered electrodes significantly increases the size of the lesions and represents a methodical optimization in our opinion.