论文信息 - Time-gated biological imaging by use of colloidal quantum dots.

Time-gated biological imaging by use of colloidal quantum dots.

The long (but not too long) fluorescence lifetime of CdSe semiconductor quantum dots was exploited to enhance fluorescence biological imaging contrast and sensitivity by time-gated detection. Significant and selective reduction of the autofluorescence contribution to the overall image was achieved, and enhancement of the signal-to-background ratio by more than an order of magnitude was demonstrated.

[1] T M Jovin,et al. Time resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging. , 1991, Biophysical journal.

[2] H Szmacinski,et al. Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics. , 1995, Biophysical journal.

[3] P. Selvin,et al. Luminescent Polyaminocarboxylate Chelates of Terbium and Europium: The Effect of Chelate Structure , 1995 .

[4] P. Selvin,et al. Crystal Structure and Spectroscopic Characterization of a Luminescent Europium Chelate , 1996 .

[5] D. Balding,et al. HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[6] S. Nie,et al. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. , 1998, Science.

[7] M. Sauer,et al. Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) at the Single Molecule Level , 2000 .