Edinburgh Research Explorer Characterization of a Cellulomonas fimi exoglucanase/xylanase-endoglucanase gene fusion which improves microbial degradation of cellulosic biomass

[1]  Sahreena S. Lakhundi,et al.  Citrobacter freundii as a test platform for recombinant cellulose degradation systems , 2017, Letters in applied microbiology.

[2]  Alistair Elfick,et al.  Fusion of pyruvate decarboxylase and alcohol dehydrogenase increases ethanol production in Escherichia coli. , 2014, ACS synthetic biology.

[3]  Song Fan,et al.  Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum β-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose , 2013, BMC Biotechnology.

[4]  K. Masaki,et al.  Fusion of cellulose binding domain from Trichoderma reesei CBHI to Cryptococcus sp. S-2 cellulase enhances its binding affinity and its cellulolytic activity to insoluble cellulosic substrates. , 2013, Enzyme and microbial technology.

[5]  G. Suen,et al.  The Genome Sequences of Cellulomonas fimi and “Cellvibrio gilvus” Reveal the Cellulolytic Strategies of Two Facultative Anaerobes, Transfer of “Cellvibrio gilvus” to the Genus Cellulomonas, and Proposal of Cellulomonas gilvus sp. nov , 2013, PloS one.

[6]  B. Pletschke,et al.  A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes--factors affecting enzymes, conversion and synergy. , 2012, Biotechnology advances.

[7]  S. Lakhundi.  Synthetic biology approach to cellulose degradation , 2012 .

[8]  A. Bommarius,et al.  The cellulose-binding domain of cellobiohydrolase Cel7A from Trichoderma reesei is also a thermostabilizing domain. , 2011, Journal of biotechnology.

[9]  L. Gomez,et al.  Sustainable liquid biofuels from biomass: the writing's on the walls. , 2008, The New phytologist.

[10]  M. Himmel,et al.  The potential of cellulases and cellulosomes for cellulosic waste management. , 2007, Current opinion in biotechnology.

[11]  S. Withers,et al.  Direct Demonstration of the Flexibility of the Glycosylated Proline-Threonine Linker in the Cellulomonas fimi Xylanase Cex through NMR Spectroscopic Analysis* , 2007, Journal of Biological Chemistry.

[12]  K. Cho,et al.  Evaluation of a novel bifunctional xylanase–cellulase constructed by gene fusion , 2005 .

[13]  E. Bayer,et al.  The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. , 2004, Annual review of microbiology.

[14]  G. Bertani,et al.  Lysogeny at Mid-Twentieth Century: P1, P2, and Other Experimental Systems , 2004, Journal of bacteriology.

[15]  A. Kosugi,et al.  Solubilization of cellulosomal cellulases by fusion with cellulose‐binding domain of noncellulosomal cellulase engd from Clostridium cellulovorans , 2003, Proteins.

[16]  Stephen C. Fry,et al.  The Growing Plant Cell Wall: Chemical and Metabolic Analysis , 2001 .

[17]  C. Haynes,et al.  Analysis of binding of the family 2a carbohydrate-binding module from Cellulomonas fimi xylanase 10A to cellulose: specificity and identification of functionally important amino acid residues. , 2000, Protein engineering.

[18]  David R. Rose,et al.  Detailed structural analysis of glycosidase/inhibitor interactions: complexes of Cex from Cellulomonas fimi with xylobiose-derived aza-sugars. , 2000, Biochemistry.

[19]  Y. Koo,et al.  Characterization of a Bifunctional Enzyme Fusion of Trehalose-6-Phosphate Synthetase and Trehalose-6-Phosphate Phosphatase of Escherichia coli , 2000, Applied and Environmental Microbiology.

[20]  E. Bayer,et al.  Cellulosomes-structure and ultrastructure. , 1998, Journal of structural biology.

[21]  E. Bayer,et al.  Cellulose, cellulases and cellulosomes. , 1998, Current opinion in structural biology.

[22]  S. Withers,et al.  Exploring the cellulose/xylan specificity of the beta-1,4-glycanase cex from Cellulomonas fimi through crystallography and mutation. , 1998, Biochemistry.

[23]  C. Haynes,et al.  Surface Diffusion of Cellulases and Their Isolated Binding Domains on Cellulose* , 1997, The Journal of Biological Chemistry.

[24]  T. Teeri,et al.  The cellulose-binding domain of the major cellobiohydrolase of Trichoderma reesei exhibits true reversibility and a high exchange rate on crystalline cellulose. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[25]  C. Haynes,et al.  Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[26]  D. Kilburn,et al.  C1-Cx revisited: intramolecular synergism in a cellulase. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[27]  P. Frey,et al.  Preparation and characterization of a bifunctional fusion enzyme composed of UDP-galactose 4-epimerase and galactose-1-P uridylyltransferase. , 1994, Bioconjugate chemistry.

[28]  S. Withers,et al.  Crystal structure of the catalytic domain of the beta-1,4-glycanase cex from Cellulomonas fimi. , 1994, Biochemistry.

[29]  N. Gilkes,et al.  Changes in the molecular-size distribution of insoluble celluloses by the action of recombinant Cellulomonas fimi cellulases. , 1994, The Biochemical journal.

[30]  S. Withers,et al.  The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants. , 1994, Biochemistry.

[31]  D. Kilburn,et al.  The cellulose‐binding domain (CBDCex) of an exoglucanase from Cellulomonas fimi: Production in Escherichia coli and characterization of the polypeptide , 1993, Biotechnology and bioengineering.

[32]  T. Reinikainen,et al.  Domain function in Trichoderma reesei cellobiohydrolases , 1992 .

[33]  K. Mosbach,et al.  Construction of an artificial bifunctional enzyme, beta-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling. , 1989, Biochemistry.

[34]  D. Kilburn,et al.  Leakage of Cellulomonas fimi cellulases from Escherichia coli , 1988 .

[35]  Tuula T. Teeri,et al.  Cellulase families and their genes , 1987 .

[36]  L. Bülow.  Characterization of an artificial bifunctional enzyme, beta-galactosidase/galactokinase, prepared by gene fusion. , 1987, European journal of biochemistry.

[37]  P. Wood,et al.  Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen , 1982, Applied and environmental microbiology.

[38]  W. D. Dotterrer,et al.  THE NUMBER OF COLONIES ALLOWABLE ON SATISFACTORY AGAR PLATES , 1916, Journal of bacteriology.

[39]  Alejandro Salinas,et al.  Beyond Genetic Engineering: Technical Capabilities in the Application Fields of Biocatalysis and Biosensors , 2015 .

[40]  Chris Somerville,et al.  Cellulosic biofuels. , 2009, Annual review of plant biology.

[41]  P. Lu,et al.  Construction and characterization of a bifunctional fusion enzyme of Bacillus-sourced beta-glucanase and xylanase expressed in Escherichia coli. , 2006, FEMS microbiology letters.

[42]  D. Gregg,et al.  Effects of sugar inhibition on cellulases and beta-glucosidase during enzymatic hydrolysis of softwood substrates. , 2004, Applied biochemistry and biotechnology.

[43]  Thomas F. Knight,et al.  Idempotent Vector Design for Standard Assembly of Biobricks , 2003 .

[44]  M. Penttilä,et al.  Structure, function, and genetics of cellulases. , 1992, Biotechnology.

[45]  K Mosbach,et al.  Multienzyme systems obtained by gene fusion. , 1991, Trends in biotechnology.

[46]  B. Henrissat,et al.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. , 1991, Microbiological reviews.

[47]  D. Kilburn,et al.  A bifunctional exoglucanase-endoglucanase fusion protein. , 1987, Gene.