论文信息 - Equine grass sickness is associated with low antibody levels to Clostridium botulinum: a matched case-control study.

Equine grass sickness is associated with low antibody levels to Clostridium botulinum: a matched case-control study.

REASONS FOR PERFORMING STUDY Equine grass sickness is a high mortality disease which, despite many years of investigation, is of unknown aetiology. Recent findings indicating that the disease is associated with Clostridium botulinum require support from an epidemiological study that recognises and controls for potential confounders, e.g. age, time of year and premises. HYPOTHESIS EGS is associated with low antibody levels to C. botulinum antigens. METHODS A matched case-control study was conducted. Data were collected from 66 histologically confirmed cases of EGS and 132 premises-matched control horses. The probability of EGS in horses was modelled using conditional logistic regression. RESULTS EGS was significantly associated (age-adjusted P < 0.005) with low antibody levels to each of 3 clostridial antigens; C. botulinum type C and C. novyi type A surface antigens and a C. botulinum type C toxin complex toxoid. These serological risk factors for EGS remained highly significant when entered into multivariable models. This study also identified new horse-level risk factors for EGS; feeding hay or haylage was associated with a decreased risk of disease, change of feed type or quantity during the 14 days prior to disease was associated with increased risk, and the use of an ivermectin anthelmintic at both the ultimate and penultimate treatments was also associated with a significantly increased risk of EGS. CONCLUSIONS This study provides strong support for the role of C. botulinum in the aetiology of EGS and identifies managemental risk factors for the disease. POTENTIAL RELEVANCE Increasing anticlostridial antibody levels by vaccination and appropriate managemental interventions may decrease the risk of EGS occurring.

[1] J. Miller,et al. Clostridia and equine grass sickness , 1997 .

[2] J L Wood,et al. A case-control study of grass sickness (equine dysautonomia) in the United Kingdom. , 1998, Veterinary journal.

[3] D. Doxey,et al. Mycological studies on the equine intestinal tract with particular reference to equine dysautonomia (grass sickness) , 1990 .

[4] C. Vaillant,et al. Diagnosis of grass sickness by ileal biopsy , 1993, Veterinary Record.

[5] I. Poxton,et al. Systemic antibodies to Clostridium botulinum type C: do they protect horses from grass sickness (dysautonomia)? , 2010, Equine veterinary journal.

[6] D. Clayton,et al. Statistical Models in Epidemiology , 1993 .

[7] H. E. McCarthy,et al. Epidemiology of equine grass sickness: a literature review (1909–1999) , 2001, Veterinary Record.

[8] P. Webbon,et al. Thirteen cases of botulism in horses fed big bale silage. , 1984, Equine veterinary journal.

[9] J. Miller,et al. The association of Clostridium botulinum type C with equine grass sickness: a toxicoinfection? , 1999, Equine veterinary journal.

[10] N. P. French,et al. Tapeworm infection is a significant risk factor for spasmodic colic and ileal impaction colic in the horse. , 1998, Equine veterinary journal.

[11] H. E. McCarthy,et al. Why are certain premises at increased risk of equine grass sickness? A matched case-control study. , 2010, Equine veterinary journal.