From one to ten in a single stroke--resolving the European Eumidasanguinea (Phyllodocidae, Annelida) species complex.

We investigate the genetic structure of European lineages of the polychaete Eumida sanguinea with mitochondrial COI and nuclear ITS, and demonstrate the presence of ten cryptic species. Within the E. sanguinea species complex there are six different white pigmentation patterns but only three of these are unique for a single species. No other consistent morphological differences were observed. We give new species names to seven of the lineages from our study, providing examples of combined morphological and molecular diagnoses, apply the available name E. sanguinea to one species, and leave two lineages with single representatives unnamed. We also include new data and a diagnosis for the poorly known E. notata, which also belongs to the E. sanguinea species complex.

[1]  U. Jondelius,et al.  Phylogenies without roots? A plea for the use of vouchers in molecular phylogenetic studies. , 2008, Molecular phylogenetics and evolution.

[2]  David B. Dunson,et al.  Bayesian Data Analysis , 2010 .

[3]  Jaa Nylander,et al.  MrModeltest 2.2. Program Distributed by the Author , 2004 .

[4]  Molecular genetic analyses of species boundaries in the sea , 2000 .

[5]  G. Rouse,et al.  Five colour morphs and three new species of Gyptis (Hesionidae, Annelida) under a jetty in Edithburgh, South Australia , 2009 .

[6]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[7]  Jerry Nedelman,et al.  Book review: “Bayesian Data Analysis,” Second Edition by A. Gelman, J.B. Carlin, H.S. Stern, and D.B. Rubin Chapman & Hall/CRC, 2004 , 2005, Comput. Stat..

[8]  G. Rouse,et al.  Reproductive biology and phylogeny of Annelida , 2006 .

[9]  John P. Huelsenbeck,et al.  MrBayes 3: Bayesian phylogenetic inference under mixed models , 2003, Bioinform..

[10]  A. Simons,et al.  The challenge of truly cryptic diversity: diagnosis and description of a new madtom catfish (Ictaluridae: Noturus) , 2006 .

[11]  G. Hartmann‐Schröder Annelida, Borstenwürmer, Polychaeta , 1996 .

[12]  R DeSalle,et al.  Alignment-ambiguous nucleotide sites and the exclusion of systematic data. , 1993, Molecular phylogenetics and evolution.

[13]  James C. Wilgenbusch,et al.  AWTY (are we there yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics , 2008, Bioinform..

[14]  Jenny Eklöf,et al.  Cryptic species of Notophyllum (Polychaeta: Phyllodocidae) in Scandinavian waters , 2010, Organisms Diversity & Evolution.

[15]  Chaolun Allen Chen,et al.  Nucleotide Sequences of Ribosomal Internal Transcribed Spacers and Their Utility in Distinguishing Closely Related Perinereis Polychaets (Annelida; Polychaeta; Nereididae) , 2002, Marine Biotechnology.

[16]  O. Hartman Catalogue of the polychaetous annelids of the world : supplement, 1960-1965, and index , 1965 .

[17]  F. Pleijel Phylogeny and classification of the Phyllodocidae (Polychaeta) , 1991 .

[18]  W. Westheide,et al.  Cosmopolitan versus cryptic meiofaunal polychaete species: an approach to a molecular taxonomy , 2003, Helgoland Marine Research.

[19]  N. Knowlton Molecular genetic analyses of species boundaries in the sea , 2000, Hydrobiologia.

[20]  C. Cazaux Recherches sur l'écologie et le développement larvaires des Polychétes de la région d'Arcachon , 1970 .

[21]  Å. Malmgren Nordiska Hafs-Annulater , 1866 .

[22]  Michael P. Cummings,et al.  PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)] , 2004 .

[23]  D. Marshall,et al.  Cryptic failure of partitioned Bayesian phylogenetic analyses: lost in the land of long trees. , 2010, Systematic biology.

[24]  David J. Lohman,et al.  Cryptic species as a window on diversity and conservation. , 2007, Trends in ecology & evolution.