Occurrence of Beet black scorch virus Infecting Sugar Beet in Europe.

In a survey of soilborne viruses infecting sugar beet in central Spain, Beet black scorch virus (BBSV) was detected in field grown sugar beets with symptoms of rhizomania disease. BBSV was found in all analyzed sugar beet producing regions from central Spain, as well as in bait plants grown in soils with a history of rhizomania from several Western European countries, thereby constituting the first report of BBSV in Europe. BBSV was transferred to Chenopodium quinoa, where it caused chlorotic local lesions from which virus particles were purified. The nucleotide sequence of the 3'-untranslated region of the genomic RNA was determined for 13 European isolates, and sequences were highly similar to those reported for Chinese and U.S. isolates. Sequence comparisons revealed three clusters of sequences, one including most European isolates, one including one European and two Chinese isolates, and the third including the U.S. isolate. BBSV was detected in a number of samples with rhizomania symptoms in which Beet necrotic yellow vein virus went undetected. However, its role in rhizomania disease in Europe, if any, remains to be established.

[1]  M. C. Edwards,et al.  Characterization of a U.S. Isolate of Beet black scorch virus. , 2007, Phytopathology.

[2]  R. Lewellen,et al.  Distribution and Molecular Characterization of Resistance-Breaking Isolates of Beet necrotic yellow vein virus in the United States. , 2007, Plant disease.

[3]  C. Rush,et al.  Mutations Associated with Resistance-Breaking Isolates of Beet necrotic yellow vein virus and Their Allelic Discrimination Using TaqMan Technology. , 2007, Phytopathology.

[4]  M. C. Edwards,et al.  First Report of Beet black scorch virus in the United States. , 2006, Plant disease.

[5]  C. Ratti,et al.  First Report of Beet virus Q in Spain. , 2006, Plant disease.

[6]  D. Gilmer,et al.  Phylogenetic analysis of isolates of Beet necrotic yellow vein virus collected worldwide. , 2005, The Journal of general virology.

[7]  J. Sears,et al.  Occurrence of Resistance-Breaking Beet necrotic yellow vein virus of Sugar Beet. , 2005, Plant disease.

[8]  Jialin Yu,et al.  Analysis of Nucleotide Sequences and Multimeric Forms of a Novel Satellite RNA Associated with Beet Black Scorch Virus , 2005, Journal of Virology.

[9]  M. Kimura.  A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences , 1980, Journal of Molecular Evolution.

[10]  J. Valkonen,et al.  Sequence Divergence of Four Soilborne Sugarbeet-Infecting Viruses , 2005, Virus Genes.

[11]  A. Schoone,et al.  Variation in Pathogenicity and Multiplication of Beet Necrotic Yellow Vein Virus (BNYVV) in Relation to the Resistance of Sugar-beet Cultivars , 1999, European Journal of Plant Pathology.

[12]  C. Rush.  Ecology and epidemiology of benyviruses and plasmodiophorid vectors. , 2003, Annual review of phytopathology.

[13]  C. Bragard,et al.  Multiplex Reverse Transcription-PCR for Simultaneous Detection of Beet Necrotic Yellow Vein Virus, Beet Soilborne Virus, and Beet Virus Q and Their Vector Polymyxa betae KESKIN on Sugar Beet , 2003, Applied and Environmental Microbiology.

[14]  N. Boonham,et al.  Occurrence in the United Kingdom of Beet necrotic yellow vein virus isolates which contain RNA 5 , 2002 .

[15]  C. Han,et al.  The complete nucleotide sequence of Beet black scorch virus (BBSV), a new member of the genus Necrovirus , 2002, Archives of Virology.

[16]  R. Koenig,et al.  Molecular analyses of European A, B and P type sources of Beet necrotic yellow vein virus and detection of the rare P type in Kazakhstan , 2000, Archives of Virology.

[17]  C. Pleij,et al.  Genome properties of beet virus Q, a new furo-like virus from sugarbeet, determined from unpurified virus. , 1998, The Journal of general virology.

[18]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[19]  U. Commandeur,et al.  Detection and Characterization of a Distinct Type of Beet Necrotic Yellow Vein Virus RNA 5 in a Sugarbeet Growing Area in Europe , 1997, Archives of Virology.

[20]  X. Cui,et al.  Study on the symptoms of rhizomania disease in sugarbeet. , 1991 .

[21]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.