Photonic gene circuits by optically addressable siRNA-Au nanoantennas.

The precise perturbation of gene circuits and the direct observation of signaling pathways in living cells are essential for both fundamental biology and translational medicine. Current optogenetic technology offers a new paradigm of optical control for cells; however, this technology relies on permanent genomic modifications with light-responsive genes, thus limiting dynamic reconfiguration of gene circuits. Here, we report precise control of perturbation and reconfiguration of gene circuits in living cells by optically addressable siRNA-Au nanoantennas. The siRNA-Au nanoantennas fulfill dual functions as selectively addressable optical receivers and biomolecular emitters of small interfering RNA (siRNA). Using siRNA-Au nanoantennas as optical inputs to existing circuit connections, photonic gene circuits are constructed in living cells. We show that photonic gene circuits are modular, enabling subcircuits to be combined on-demand. Photonic gene circuits open new avenues for engineering functional gene circuits useful for fundamental bioscience, bioengineering, and medical applications.

[1]  F. Thibaudau.  Ultrafast Photothermal Release of DNA from Gold Nanoparticles. , 2012, The journal of physical chemistry letters.

[2]  B. Dragnea,et al.  Photothermal imaging and measurement of protein shell stoichiometry of single HIV-1 Gag virus-like nanoparticles. , 2011, ACS nano.

[3]  Peter Nordlander,et al.  Light-induced release of DNA from gold nanoparticles: nanoshells and nanorods. , 2011, Journal of the American Chemical Society.

[4]  T. Niidome,et al.  Controlled-release system of single-stranded DNA triggered by the photothermal effect of gold nanorods and its in vivo application. , 2011, Bioorganic & medicinal chemistry.

[5]  Erika Pastrana,et al.  Optogenetics: controlling cell function with light , 2011, Nature Methods.

[6]  Dipankar Sen,et al.  Photothermal release of single-stranded DNA from the surface of gold nanoparticles through controlled denaturating and Au-S bond breaking. , 2010, ACS nano.

[7]  Chen-Yuan Dong,et al.  Multiple release kinetics of targeted drug from gold nanorod embedded polyelectrolyte conjugates induced by near-infrared laser irradiation. , 2010, Journal of the American Chemical Society.

[8]  Luke P. Lee,et al.  Nanoplasmonic gene regulation. , 2010, Current opinion in chemical biology.

[9]  Wei Lu,et al.  Tumor Site–Specific Silencing ofNF-κB p65by Targeted Hollow Gold Nanosphere–Mediated Photothermal Transfection , 2010, Cancer Research.

[10]  W. Cai,et al.  Plasmonics for extreme light concentration and manipulation. , 2010, Nature materials.

[11]  Naomi J. Halas,et al.  Light-induced release of DNA from plasmon-resonant nanoparticles: Towards light-controlled gene therapy , 2009 .

[12]  Kaylie L. Young,et al.  Plasmonically controlled nucleic acid dehybridization with gold nanoprisms. , 2009, Chemphyschem : a European journal of chemical physics and physical chemistry.

[13]  Matthew Tirrell,et al.  Laser-Activated Gene Silencing via Gold Nanoshell-siRNA Conjugates. , 2009, ACS nano.

[14]  Galit Lahav,et al.  The ups and downs of p53: understanding protein dynamics in single cells , 2009, Nature Reviews Cancer.

[15]  K. Hamad-Schifferli,et al.  Selective release of multiple DNA oligonucleotides from gold nanorods. , 2009, ACS nano.

[16]  Luke P. Lee,et al.  Remote optical switch for localized and selective control of gene interference. , 2009, Nano letters.

[17]  Luke P. Lee,et al.  Biologically Functional Cationic Phospholipid-Gold Nanoplasmonic Carriers , 2009 .

[18]  Naomi J Halas,et al.  Nanoshell-enabled photothermal cancer therapy: impending clinical impact. , 2008, Accounts of chemical research.

[19]  Travis L. Jennings,et al.  Enhancing the Toxicity of Cancer Chemotherapeutics with Gold Nanorod Hyperthermia , 2008 .

[20]  R. K. Harrison,et al.  Thermal analysis of gold nanorods heated with femtosecond laser pulses , 2008, Journal of physics D: Applied physics.

[21]  Nanfang Yu,et al.  Plasmonic Laser Antennas and Related Devices , 2008, IEEE Journal of Selected Topics in Quantum Electronics.

[22]  Daniel Day,et al.  Cancer cell imaging and photothermal therapy using gold nanorods , 2008 .

[23]  Mark E. Davis,et al.  Effect of siRNA nuclease stability on the in vitro and in vivo kinetics of siRNA‐mediated gene silencing , 2007, Biotechnology and bioengineering.

[24]  A Paul Alivisatos,et al.  A nanoplasmonic molecular ruler for measuring nuclease activity and DNA footprinting , 2006, Nature nanotechnology.

[25]  Valery V. Tuchin,et al.  Optical amplification of photothermal therapy with gold nanoparticles and nanoclusters , 2006 .

[26]  Uri Alon,et al.  An Introduction to Systems Biology , 2006 .

[27]  Chad A. Mirkin,et al.  Oligonucleotide-Modified Gold Nanoparticles for Intracellular Gene Regulation , 2006, Science.

[28]  Xiaohua Huang.  Gold Nanoparticles Used in Cancer Cell Diagnostics, Selective Photothermal Therapy and Catalysis of NADH Oxidation Reaction , 2006 .

[29]  Yi-Cheng Chen,et al.  DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation. , 2006, Journal of the American Chemical Society.

[30]  Luke P. Lee,et al.  Optofluidic control using photothermal nanoparticles , 2006, Nature materials.

[31]  Dieter Braun,et al.  The role of metal nanoparticles in remote release of encapsulated materials. , 2005, Nano letters.

[32]  Catherine J. Murphy,et al.  Fine-tuning the shape of gold nanorods , 2005 .

[33]  Carsten Sönnichsen,et al.  A molecular ruler based on plasmon coupling of single gold and silver nanoparticles , 2005, Nature Biotechnology.

[34]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[35]  B. Nikoobakht,et al.  種結晶を媒介とした成長法を用いた金ナノロッド(NR)の調製と成長メカニズム , 2003 .

[36]  A. Hoffmann,et al.  The I (cid:1) B –NF-(cid:1) B Signaling Module: Temporal Control and Selective Gene Activation , 2022 .

[37]  Dieter Braun,et al.  Trapping of DNA by thermophoretic depletion and convection. , 2002, Physical review letters.

[38]  T. Libermann,et al.  Adenovirus vector-induced inflammation: capsid-dependent induction of the C-C chemokine RANTES requires NF-kappa B. , 2002, Human gene therapy.

[39]  K. Hamad-Schifferli,et al.  Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna , 2002, Nature.

[40]  Carsten Sönnichsen,et al.  Plasmons in metal nanostructures , 2001 .

[41]  M El Sayed,et al.  SHAPE AND SIZE DEPENDENCE OF RADIATIVE, NON-RADIATIVE AND PHOTOTHERMAL PROPERTIES OF GOLD NANOCRYSTALS , 2000 .

[42]  K. Svoboda,et al.  Biological applications of optical forces. , 1994, Annual review of biophysics and biomolecular structure.