Studies on anti-component 5 antibodies in animals infected with Trypanosoma cruzi.

A competitive antibody enzyme immunoassay, using a monoclonal antibody against the species-specific Trypanosoma cruzi antigen 5, was used to investigate the presence o f anti-component 5 antibodies in sera of opossums, dogs, rabbits and rats infected with this parasite. The sera from 51 Venezuelan patients with Chagas’disease were also tested. About 90% of the infected subjects showed significant levels o f anti-component 5 antibodies. Nevertheless, these antibodies were not detected in the sera o f dogs, rats and opossums infected with T. cruzi. Some sera from infected rabbits presented significant results but close to the limit o f positivity o f the test. Thesefindings suggest that the immune response in animals naturally or experimentally infected with T. cruzi is different from that observed in human Chagas’disease.

[1]  M. Loyens,et al.  Specific and sensitive immunological diagnosis of Chagas' disease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi-specific monoclonal antibody. , 1986, The American journal of tropical medicine and hygiene.

[2]  A. Capron,et al.  Subspecies-specific surface antigens of promastigotes of the Leishmania donovani complex , 1985, Infection and immunity.

[3]  A. Capron,et al.  Different monoclonal antibodies against the component 5 specific for Trypanosoma cruzi. , 1984, The American journal of tropical medicine and hygiene.

[4]  S. G. Andrade,et al.  Damage and healing in the conducting tissue of the heart (an experimental study in dogs infected with Trypanosoma cruzi) , 1984, The Journal of pathology.

[5]  A. Sher,et al.  Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody. , 1984, Molecular and biochemical parasitology.

[6]  M. Miles,et al.  PURIFIED TRYPANOSOMA CRUZI SPECIFIC GLYCOPROTEIN FOR DISCRIMINATIVE SEROLOGICAL DIAGNOSIS OF SOUTH AMERICAN TRYPANOSOMIASIS (CHAGAS' DISEASE) , 1983, The Lancet.

[7]  J. Scharfstein,et al.  Trypanosoma cruzi: description of a highly purified surface antigen defined by human antibodies. , 1983, Journal of immunology.

[8]  P. Desjeux,et al.  Human visceral leishmaniasis in Bolivia: first proven autochthonous case from 'Los Yungas'. , 1983, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[9]  D. Modai,et al.  Iatrogenic arterial aneurysm associated with ergotamine therapy. , 1981, Lancet.

[10]  M. C. Guimarães,et al.  Immunoenzymatic assy (ELISA) in mucocutaneous leishmaniasis, kala-azar, and Chagas' disease: an epimastigote Trypanosoma cruzi antigen able to distinguish between anti-Trypanosoma and anti-Leishmania antibodies. , 1981, The American journal of tropical medicine and hygiene.

[11]  A. Capron,et al.  Trypanosoma cruzi infection in B-cell-deficient rats , 1981, Infection and immunity.

[12]  E. L. Segura,et al.  Diferencias y similitudes antigénicas entre T. rangeli y T. cruzi , 1980 .

[13]  A. Capron,et al.  Antigenic make-up of Trypanosoma cruzi culture forms: identification of a specific component. , 1979, The Journal of parasitology.

[14]  A. Capron,et al.  Purification of a specific antigen of Trypanosoma cruzi from culture forms. , 1978, The American journal of tropical medicine and hygiene.

[15]  P. Desjeux,et al.  [On 113 cases of cutaneous leishmaniasis and mucocutaneous leishmaniasis observed in Bolivia. Sero-immunologic study of 71 cases]. , 1974, Bulletin de la Societe de pathologie exotique et de ses filiales.

[16]  D. Wegner,et al.  A comparison of a new antigen from amastigotes of Trypanosoma cruzi and an antigen from epimastigotes for the diagnosis of Chagas' disease by the indirect immunofluorescence test. , 1971, Revista do Instituto de Medicina Tropical de Sao Paulo.

[17]  C. M. Johnson,et al.  Frequency and distribution of Trypanosoma cruzi and Trypanosoma rangeli in the Republic of Panamá. , 1971, The American journal of tropical medicine and hygiene.

[18]  M. Camargo,et al.  Cross-reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies. A simple inhibition procedure to ensure specific results. , 1969, The American journal of tropical medicine and hygiene.

[19]  S. Avrameas,et al.  Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. , 1969, Immunochemistry.