Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source.

We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

[1]  Y. Nishizuka,et al.  The protein kinase C family for neuronal signaling. , 1994, Annual review of neuroscience.

[2]  R Gauderon,et al.  Three-dimensional second-harmonic generation imaging with femtosecond laser pulses. , 1998, Optics letters.

[3]  Hall,et al.  Carrier-envelope phase control of femtosecond mode-locked lasers and direct optical frequency synthesis , 2000, Science.

[4]  R. Leonhardt,et al.  Supercontinuum generation by stimulated Raman scattering and parametric four-wave mixing in photonic crystal fibers , 2002 .

[5]  Hiroyuki Yokoyama,et al.  Two-photon bioimaging with picosecond optical pulses from a semiconductor laser. , 2006, Optics express.

[6]  T. Okada,et al.  Novel Er and Ce codoped fluoride fiber amplifier for low-noise and high-efficient operation with 980-nm pumping , 2003, IEEE Photonics Technology Letters.

[7]  S. Davey,et al.  High output power from an efficient praseodymium-doped fluoride fiber amplifier , 1992, IEEE Photonics Technology Letters.

[8]  R. Tyson.  Bit-error rate for free-space adaptive optics laser communications. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[9]  Kazuyoshi Itoh,et al.  Multi-Spectral Two-Photon Excited Fluorescence Microscopy Using Supercontinuum Light Source , 2005 .

[10]  Hiroyuki Yokoyama,et al.  Two-photon fluorescence imaging with a pulse source based on a 980-nm gain-switched laser diode. , 2007, Optics express.

[11]  Knight,et al.  Optical frequency synthesizer for precision spectroscopy , 2000, Physical review letters.

[12]  G Korn,et al.  Experimental evidence for supercontinuum generation by fission of higher-order solitons in photonic fibers. , 2002, Physical review letters.

[13]  Haruo Kasai,et al.  Propagation of γPKC translocation along the dendrites of Purkinje cell in γPKC‐GFP transgenic mice , 2004 .

[14]  William J. Wadsworth,et al.  Soliton effects in photonic crystal fibres at 850 nm , 2000 .

[15]  H. Ito,et al.  High-peak-power picosecond optical-pulse generation with a gain-switched semiconductor laser, and high-efficiency wavelength conversion , 2005, 2005 Pacific Rim Conference on Lasers & Electro-Optics.

[16]  W. Webb,et al.  Nonlinear magic: multiphoton microscopy in the biosciences , 2003, Nature Biotechnology.

[17]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[18]  D. Hanna,et al.  Tunable, continuous-wave neodymium-doped monomode-fiber laser operating at 0.900-0.945 and 1.070-1.135 microm. , 1986, Optics letters.

[19]  Milton Feng,et al.  AlGaN/GaN heterojunction bipolar transistors grown by metal organic chemical vapour deposition , 2000 .

[20]  Shaif-ul Alam,et al.  High-power wavelength-tunable cladding-pumped rare-earth-doped silica fiber lasers , 2004 .

[21]  Toshio Morioka,et al.  Transform-limited, femtosecond WDM pulse generation by spectral filtering of gigahertz supercontinuum , 1994 .

[22]  Toshio Morioka,et al.  Flatly broadened supercontinuum spectrum generated in a dispersion decreasing fibre with convex dispersion profile , 1997 .

[23]  W. Denk,et al.  Two-photon laser scanning fluorescence microscopy. , 1990, Science.

[24]  W. Webb,et al.  Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation , 1997, Science.

[25]  P. A. Tick,et al.  Efficient neodymium-doped glass-ceramic fiber laser and amplifier. , 2001, Optics letters.

[26]  J. Thøgersen,et al.  Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..

[27]  Yaron Silberberg,et al.  Nonlinear scanning laser microscopy by third harmonic generation , 1997 .