Curium(III) Speciation Studies with Cells of a Groundwater Strain of Pseudomonas fluorescens

Ubiquitous Pseudomonads have great potential to influence the speciation and mobility of actinides in the environment. This study explores the unknown interaction between curium(III) and cell-suspensions of Pseudomonas fluorescens (CCUG 32456) isolated from the Äspö site, Sweden. The interaction between curium(III) and P. fluorescens cells was studied at trace curium(III) concentrations (0.3 μM) using time-resolved laser-induced fluorescence spectroscopy. Extraction studies have shown that the biosorption of curium(III) is a reversible process. Two Cm3+−P. fluorescens (CCUG 32456) species were identified, R−O−PO3H−Cm2+ and R−COO−Cm2+, having emission maxima at 599.6 and 601.9 nm, respectively. The corresponding surface complexation constants were determined to be log β111 = 12.7 ± 0.6 and log β110 = 6.1 ± 0.5, respectively.

[1]  G. Bernhard,et al.  A TRLFS study of curium(III) naphthalene and hydroxyquinoline complexes in aqueous solution , 2012 .

[2]  C. Anderson,et al.  Radionuclide Geomicrobiology of the Deep Biosphere , 2011 .

[3]  J. Lloyd,et al.  The Geomicrobiology of Radionuclides , 2011 .

[4]  G. Bernhard,et al.  Complexation study of europium(III) and curium(III) with urea in aqueous solution investigated by time-resolved laser-induced fluorescence spectroscopy , 2009 .

[5]  G. Bernhard,et al.  Complexation of curium(III) with hydroxamic acids investigated by time-resolved laser-induced fluorescence spectroscopy , 2008 .

[6]  M. Maedera,et al.  The Investigation of Equilibria in Solution , 2008 .

[7]  M. Schäfer,et al.  Curium(III) complexation with pyoverdins secreted by a groundwater strain of Pseudomonas fluorescens , 2008, BioMetals.

[8]  A. Francis,et al.  Effects of ionic strength on the coordination of Eu(III) and Cm(III) to a Gram-negative bacterium, Paracoccus denitrificans , 2006 .

[9]  N. Edelstein,et al.  Optical properties of Cm(III) in crystals and solutions and their application to Cm(III) speciation , 2006 .

[10]  G. Choppin,et al.  Complexation thermodynamics and structural aspects of actinide–aminopolycarboxylates , 2006 .

[11]  B. Ahmed,et al.  Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens , 2005 .

[12]  G. Bernhard,et al.  Complexation of curium(III) by adenosine 5′-triphosphate (ATP): A time-resolved laser-induced fluorescence spectroscopy (TRLFS) study , 2005 .

[13]  A. Roßberg,et al.  Time-resolved laser fluorescence spectroscopy study on the interaction of curium(III) with Desulfovibrio äspöensis DSM 10631T. , 2004, Environmental science & technology.

[14]  A. Francis,et al.  Sorption behavior of europium(III) and curium(III) on the cell surfaces of microorganisms , 2004 .

[15]  A. Francis,et al.  Interactions of trivalent and tetravalent heavy metal-siderophore complexes with Pseudomonas fluorescens , 2004 .

[16]  H. Budzikiewicz.  Siderophores of the Pseudomonadaceae sensu stricto (fluorescent and non-fluorescent Pseudomonas spp.). , 2004, Fortschritte der Chemie organischer Naturstoffe = Progress in the chemistry of organic natural products. Progres dans la chimie des substances organiques naturelles.

[17]  A. Francis,et al.  Association mechanisms of europium(III) and curium(III) with Chlorella vulgaris , 2003, Environmental toxicology and chemistry.

[18]  Y. Andres,et al.  Study of the interaction between europium (III) and Bacillus subtilis: fixation sites, biosorption modeling and reversibility. , 2003, Journal of colloid and interface science.

[19]  R. Guillaumont,et al.  Update on the chemical thermodynamics of uranium, neptunium, plutonium, americium and technetium , 2003 .

[20]  P. Panak,et al.  Interaction of aerobic soil bacteria with plutonium(VI) , 2001 .

[21]  D. S. Smith,et al.  Characterizing Heterogeneous Bacterial Surface Functional Groups Using Discrete Affinity Spectra for Proton Binding , 1999 .

[22]  K. Pedersen.  Microbial life in deep granitic rock , 1997 .

[23]  P. Gans,et al.  Investigation of equilibria in solution. Determination of equilibrium constants with the HYPERQUAD suite of programs. , 1996, Talanta.

[24]  A. Albrecht-Gary,et al.  BACTERIAL IRON TRANSPORT : COORDINATION PROPERTIES OF PYOVERDIN PAA, A PEPTIDIC SIDEROPHORE OF PSEUDOMONAS AERUGINOSA , 1994 .

[25]  G. Choppin,et al.  Luminescence study on determination of the hydration number of Cm(III) , 1994 .

[26]  J. I. Kim,et al.  Complexation of Trivalent Actinide Ions (Am3+, Cm3+) with Humic Acid: A Comparison of Different Experimental Methods , 1993 .

[27]  C. Reid,et al.  Occurrence of hydroxamate siderophore iron chelators in soils , 1980, Nature.