A Single Nucleotide Polymorphism (SNP) c.20G>A in somatostatin gene (SST|BsrT) on Madura cattle

Madura cattle is one of the local cattle in Indonesia that have the various phenotype, especially in growth. The objective of this study was to identify SNP of Somatostatin gene on Madura cattle at Village Breeding Centre (VBC), Madura Island. A total of 66 Madura cattle was used in this study and 34 Ongole Grade cattle as a population reference. Blood samples were collected at VBC Waru, Pamekasan Regency. A potential SNP in exon 1 was analyzed using Polymerase Chain Reaction-Restricted Fragment Length Polymorphism (PCR-RFLP). A restriction enzyme of BsrI was used to identify the SNP. The results showed that a 464 bp of DNA fragment was successfully amplified and an SNP c.20G>A was identified in this study. This SNP was categorized as a missense mutation with the change of amino acid from serine (AGT) to asparagine (AAT). An A allele was only found on Madura cattle. The frequency of A allele was 1.00 in this population and was defined as monomorphic or monoallelic. This result probably indicated that Madura cattle at VBC Waru were selected in this locus.

[1]  Muladno,et al.  Investigating new SNPs of CAST, CAPN and SCD genes in 5’UTR of Bali cattle , 2020, Biodiversitas Journal of Biological Diversity.

[2]  Emmanuel Ampofo,et al.  Regulatory Mechanisms of Somatostatin Expression , 2020, International journal of molecular sciences.

[3]  M. Trau,et al.  PrimerROC: accurate condition-independent dimer prediction using ROC analysis , 2019, Scientific Reports.

[4]  F. Guan,et al.  A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification , 2018, Journal of analytical methods in chemistry.

[5]  N. Ulupi,et al.  Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross , 2017 .

[6]  M. F. Johan,et al.  Guidelines for nucleic acid detection and analysis in hematological disorders. , 2015, The Malaysian journal of pathology.

[7]  Ying Yu,et al.  Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk. , 2014, Genetics and molecular research : GMR.

[8]  J. Aleksić,et al.  A Simple and Efficient DNA Isolation Method for Salvia officinalis , 2012, Biochemical Genetics.

[9]  L. Zan,et al.  Polymorphism of somatostatin gene and its association with growth traits in Chinese cattle. , 2011, Genetics and molecular research : GMR.

[10]  Chiwook Park,et al.  Revisiting absorbance at 230nm as a protein unfolding probe. , 2009, Analytical biochemistry.

[11]  J. Holst,et al.  Vagal control of the release of somatostatin, vasoactive intestinal polypeptide, gastrin-releasing peptide, and HCl from porcine non-antral stomach. , 1992, Scandinavian journal of gastroenterology.