Comparative study using spheres, rods and spindle-shaped nanoplatelets on dispersion stability, dissolution and toxicity of CuO nanomaterials

Abstract Copper oxide nanoparticles with different shapes were used to examine the effect of shape on the various physicochemical properties (reactivity, aggregation, suspension stability) and to examine the behaviour by which CuO nanoparticles exhibit their biological response towards alveolar type-I cells. The different shapes examined in this study include spherical-, rod- and spindle-shaped platelet particles. In vitro dissolution studies (7 days) in 1 mM NaNO3 matrix showed a marked difference in dissolved Cu release between the nanoparticles. However, in serum-free cell-culture media (exposure media to cells), the particles' dissolution was found to be significantly enhanced with close to complete dissolution reported for all particle types. Biological studies showed both shape and size of the CuO nanoparticles tested to have a significant effect on TT-1 cell viability and release of pro-inflammatory cytokines IL-6 and IL-8. This study shows a complex interplay between particulate and dissolved species triggering the biological response. Upon immediate exposure of CuO nanoparticles of different shapes, the particulate form contributes towards the toxicity. However, for any biological response observed over and beyond a period of 24 h, the dissolved fraction becomes significant.

[1]  Xujie Yang,et al.  Highly dispersed CuO nanoparticles prepared by a novel quick-precipitation method , 2004 .

[2]  M. Almukainzi,et al.  Simulated Biological Fluids with Possible Application in Dissolution Testing , 2011 .

[3]  A. Sass-kortsak,et al.  The state of copper in human serum: evidence for an amino acid-bound fraction. , 1967, The Journal of clinical investigation.

[4]  Enrique Navarro,et al.  Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. , 2008, Environmental science & technology.

[5]  Deborah Berhanu,et al.  The complexity of nanoparticle dissolution and its importance in nanotoxicological studies. , 2012, The Science of the total environment.

[6]  A. Ponce,et al.  Imaging interactions of metal oxide nanoparticles with macrophage cells by ultra-high resolution scanning electron microscopy techniques. , 2012, Integrative biology : quantitative biosciences from nano to macro.

[7]  Christofer Leygraf,et al.  Surface characteristics, copper release, and toxicity of nano- and micrometer-sized copper and copper(II) oxide particles: a cross-disciplinary study. , 2009, Small.

[8]  Stephanie E. A. Gratton,et al.  The effect of particle design on cellular internalization pathways , 2008, Proceedings of the National Academy of Sciences.

[9]  R. Hurt,et al.  Controlled release of biologically active silver from nanosilver surfaces. , 2010, ACS nano.

[10]  G. E. Gadd,et al.  Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata): the importance of particle solubility. , 2007, Environmental science & technology.

[11]  H. Karlsson,et al.  Size-dependent toxicity of metal oxide particles--a comparison between nano- and micrometer size. , 2009, Toxicology letters.

[12]  P. Costa,et al.  Modeling and comparison of dissolution profiles. , 2001, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[13]  Wei Wang,et al.  Toxicity of amorphous silica nanoparticles in mouse keratinocytes , 2009 .

[14]  Holger Moch,et al.  Nanoparticle cytotoxicity depends on intracellular solubility: comparison of stabilized copper metal and degradable copper oxide nanoparticles. , 2010, Toxicology letters.

[15]  Youn-Joo An,et al.  Microbial toxicity of metal oxide nanoparticles (CuO, NiO, ZnO, and Sb2O3) to Escherichia coli, Bacillus subtilis, and Streptococcus aureus. , 2011, The Science of the total environment.

[16]  J. Song,et al.  Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli , 2007, Applied and Environmental Microbiology.

[17]  Albert Duschl,et al.  Shape matters: effects of silver nanospheres and wires on human alveolar epithelial cells , 2011, Particle and Fibre Toxicology.

[18]  R. Amal,et al.  Cytotoxic origin of copper(II) oxide nanoparticles: comparative studies with micron-sized particles, leachate, and metal salts. , 2011, ACS nano.

[19]  Kenneth A. Dawson,et al.  Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts , 2008, Proceedings of the National Academy of Sciences.

[20]  R. Hurt,et al.  Ion release kinetics and particle persistence in aqueous nano-silver colloids. , 2010, Environmental science & technology.

[21]  Paula T Hammond,et al.  The effects of polymeric nanostructure shape on drug delivery. , 2011, Advanced drug delivery reviews.

[22]  A. Boccaccini,et al.  Isotopically modified nanoparticles for enhanced detection in bioaccumulation studies. , 2012, Environmental science & technology.

[23]  W. Lee,et al.  Effects of pH variation in aqueous solutions on dissolution of copper oxide , 2010 .

[24]  S. Cormier,et al.  Copper oxide nanoparticles induce oxidative stress and cytotoxicity in airway epithelial cells. , 2009, Toxicology in vitro : an international journal published in association with BIBRA.

[25]  H. Kim,et al.  Size-dependent cellular toxicity of silver nanoparticles. , 2012, Journal of biomedical materials research. Part A.

[26]  Qingxiu Wang,et al.  Integrated metabolomic analysis of the nano-sized copper particle-induced hepatotoxicity and nephrotoxicity in rats: a rapid in vivo screening method for nanotoxicity. , 2008, Toxicology and applied pharmacology.

[27]  A. Mills,et al.  PHOTOMINERALIZATION OF 4-CHLOROPHENOL SENSITIZED BY TITANIUM-DIOXIDE - A STUDY OF THE INITIAL KINETICS OF CARBON-DIOXIDE PHOTOGENERATION , 1993 .

[28]  B. Liao,et al.  Zeta potential of shape-controlled TiO2 nanoparticles with surfactants , 2009 .

[29]  Monika Mortimer,et al.  Toxicity of ZnO and CuO nanoparticles to ciliated protozoa Tetrahymena thermophila. , 2010, Toxicology.

[30]  Dong Chen,et al.  The effect of the shape of mesoporous silica nanoparticles on cellular uptake and cell function. , 2010, Biomaterials.

[31]  Iqbal Ahmad,et al.  Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells. , 2010, Biochemical and biophysical research communications.

[32]  Younan Xia,et al.  The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles. , 2011, Nature nanotechnology.

[33]  Yongsheng Chen,et al.  Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. , 2012, ACS nano.

[34]  Pratim Biswas,et al.  Role of Surface Area, Primary Particle Size, and Crystal Phase on Titanium Dioxide Nanoparticle Dispersion Properties , 2010, Nanoscale research letters.

[35]  H. Karlsson,et al.  Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes. , 2008, Chemical research in toxicology.

[36]  Thilini P. Rupasinghe,et al.  Aggregation and dissolution of 4 nm ZnO nanoparticles in aqueous environments: influence of pH, ionic strength, size, and adsorption of humic acid. , 2011, Langmuir : the ACS journal of surfaces and colloids.

[37]  Kyunghee Choi,et al.  Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. , 2010, Toxicology in vitro : an international journal published in association with BIBRA.

[38]  M. Mortimer,et al.  Ecotoxicity of nanoparticles of CuO and ZnO in natural water. , 2010, Environmental pollution.