In vivo study of nanostructured diopside (CaMgSi2O6) coating on magnesium alloy as biodegradable orthopedic implants

Abstract In order to improve the corrosion resistance and bioactivity of a biodegradable magnesium alloy, we have recently prepared a nanostructured diopside (CaMgSi2O6) coating on AZ91 magnesium alloy through a combined micro-arc oxidation (MAO) and electrophoretic deposition (EPD) method (reported elsewhere). In this work, we performed a detailed biocompatibility analysis of the implants made by this material and compared their performance with those of the uncoated and micro arc oxidized magnesium implants. The biocompatibility evaluation of samples was performed by culturing L-929 cells and in vivo animal study, including implantation of samples in greater trochanter of rabbits, radiography and histological examinations. The results from both the in vitro and in vivo studies indicated that the diopside/MAO coated magnesium implant significantly enhanced cell viability, biodegradation resistance and new bone formation compared with both the uncoated and the micro-arc oxidized magnesium implants. Our data provides an example of how the proper surface treatment of magnesium implants can overcome their drawbacks in terms of high degradation rate and gas bubble formation under physiological conditions.

[1]  A R Boccaccini,et al.  Biomedical coatings on magnesium alloys - a review. , 2012, Acta biomaterialia.

[2]  M. Razavi,et al.  Biodegradation, Bioactivity and In vivo Biocompatibility Analysis of Plasma Electrolytic Oxidized (PEO) Biodegradable Mg Implants , 2014 .

[3]  M. Fathi,et al.  Fabrication and characterization of magnesium-fluorapatite nanocomposite for biomedical applications , 2010 .

[4]  Ke Yang,et al.  Fluoride treatment and in vitro corrosion behavior of an AZ31B magnesium alloy , 2010 .

[5]  M. Fathi,et al.  Preparation and characterization of sol–gel bioactive glass coating for improvement of biocompatibility of human body implant , 2008 .

[6]  D. Vashaee,et al.  Multilayer zirconium titanate thin films prepared by a sol-gel deposition method , 2013 .

[7]  M. Fathi,et al.  A REVIEW OF DEGRADATION PROPERTIES OF Mg BASED BIODEGRADABLE IMPLANTS , 2012 .

[8]  J. Nellesen,et al.  Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling. , 2010, Acta biomaterialia.

[9]  D. Vashaee,et al.  Aqueous sol–gel synthesis of zirconium titanate (ZrTiO4) nanoparticles using chloride precursors , 2012 .

[10]  M. Fathi,et al.  Bio-corrosion behavior of magnesium-fluorapatite nanocomposite for biomedical applications , 2010 .

[11]  Y. Miake,et al.  High-resolution and Analytical Electron Microscopic Studies of New Crystals Induced by a Bioactive Ceramic (Diopside) , 1995, Journal of dental research.

[12]  D. Vashaee,et al.  Micro‐arc oxidation and electrophoretic deposition of nano‐grain merwinite (Ca3MgSi2O8) surface coating on magnesium alloy as biodegradable metallic implant , 2014 .

[13]  J. Voegel,et al.  Influence of magnesium substitution on a collagen-apatite biomaterial on the production of a calcifying matrix by human osteoblasts. , 1998, Journal of biomedical materials research.

[14]  D. Vashaee,et al.  Electroconductive Nanocomposite Scaffolds: A New Strategy Into Tissue Engineering and Regenerative Medicine , 2012 .

[15]  D. Vashaee,et al.  Multilayer bioactive glass/zirconium titanate thin films in bone tissue engineering and regenerative dentistry , 2013, International journal of nanomedicine.

[16]  Yufeng Zheng,et al.  The development of binary Mg-Ca alloys for use as biodegradable materials within bone. , 2008, Biomaterials.

[17]  D. Vashaee,et al.  Controlling the degradation rate of bioactive magnesium implants by electrophoretic deposition of akermanite coating , 2014 .

[18]  Csaba Vermes,et al.  Concentration- and composition-dependent effects of metal ions on human MG-63 osteoblasts. , 2002, Journal of biomedical materials research.

[19]  Larry L. Hench,et al.  An Introduction to Bioceramics , 2013 .

[20]  D. Vashaee,et al.  Surface modification of magnesium alloy implants by nanostructured bredigite coating , 2013 .

[21]  Ralf Rettig,et al.  Composition of corrosion layers on a magnesium rare-earth alloy in simulated body fluids. , 2009, Journal of biomedical materials research. Part A.

[22]  Alexis M Pietak,et al.  Magnesium and its alloys as orthopedic biomaterials: a review. , 2006, Biomaterials.

[23]  J. Weng,et al.  Characterization of titanium surfaces with calcium and phosphate and osteoblast adhesion. , 2004, Biomaterials.

[24]  M. Fathi,et al.  Novel magnesium-nanofluorapatite metal matrix nanocomposite with improved biodegradation behavior. , 2011, Journal of biomedical nanotechnology.

[25]  Mitsuo Niinomi,et al.  Recent metallic materials for biomedical applications , 2002 .

[26]  G. Song,et al.  The anodic dissolution of magnesium in chloride and sulphate solutions , 1997 .

[27]  M. Mozafari,et al.  Enhancement of fracture toughness in bioactive glass-based nanocomposites with nanocrystalline forsterite as advanced biomaterials for bone tissue engineering applications , 2012 .

[28]  Ke Yang,et al.  In vivo corrosion behavior of Mg-Mn-Zn alloy for bone implant application. , 2007, Journal of biomedical materials research. Part A.

[29]  Saied Nouri Khorasani,et al.  Effect of Surface Treatment and Metallic Coating on Corrosion Behavior and Biocompatibility of Surgical 316L Stainless Steel Implant , 2012 .

[30]  Keith D K Luk,et al.  A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants. , 2010, Biomaterials.

[31]  J. Kerstetter,et al.  Nutrition in Bone Health Revisited: A Story Beyond Calcium , 2000, Journal of the American College of Nutrition.

[32]  S. Madihally,et al.  Hybrid macroporous gelatin/bioactive-glass/nanosilver scaffolds with controlled degradation behavior and antimicrobial activity for bone tissue engineering. , 2014, Journal of biomedical nanotechnology.

[33]  S. Madihally,et al.  3D conductive nanocomposite scaffold for bone tissue engineering , 2013, International journal of nanomedicine.

[34]  Guang-Ling Song,et al.  Control of biodegradation of biocompatable magnesium alloys , 2007 .

[35]  M. Fathi,et al.  Novel hydroxyapatite/tantalum surface coating for metallic dental implant , 2007 .

[36]  S. Tsutsumi,et al.  Study of diopside ceramics for biomaterials , 1999, Journal of materials science. Materials in medicine.

[37]  Anil Kumar,et al.  CELL SURFACE INTERACTIONS IN THE STUDY OF BIOCOMPATIBILITY , 2002 .

[38]  Seyed Mohammad Ali Razavi,et al.  Coating of biodegradable magnesium alloy bone implants using nanostructured diopside (CaMgSi2O6) , 2014 .

[39]  M. Fathi,et al.  Microstructure, mechanical properties and bio-corrosion evaluation of biodegradable AZ91-FA nanocomposites for biomedical applications , 2010 .

[40]  D. Vashaee,et al.  Nanostructured zirconium titanate fibers prepared by particulate sol–gel and cellulose templating techniques , 2013 .

[41]  H. Haferkamp,et al.  In vivo corrosion of four magnesium alloys and the associated bone response. , 2005, Biomaterials.

[42]  Frank Witte,et al.  In vitro and in vivo corrosion measurements of magnesium alloys. , 2006, Biomaterials.

[43]  S. Madihally,et al.  In Vitro Electrochemical Corrosion and Cell Viability Studies on Nickel-Free Stainless Steel Orthopedic Implants , 2013, PloS one.

[44]  M. Fathi,et al.  In vitro corrosion behavior of bioceramic, metallic, and bioceramic-metallic coated stainless steel dental implants. , 2003, Dental materials : official publication of the Academy of Dental Materials.