Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by flow cytometry: Methodology and new insights

The need to accurately discriminate dendritic cells (DCs) and macrophages (Mphs) in mouse lungs is critical given important biological differences. However, a validated flow cytometry–based method is still lacking, resulting in much confusion between both cell types.

[1]  M. Pouladi,et al.  Cigarette smoke decreases pulmonary dendritic cells and impacts antiviral immune responsiveness. , 2004, American journal of respiratory cell and molecular biology.

[2]  G. Trinchieri,et al.  Mouse Strain Differences in Plasmacytoid Dendritic Cell Frequency and Function Revealed by a Novel Monoclonal Antibody , 2003, Journal of Immunology.

[3]  R. Pauwels,et al.  Accelerated airway dendritic cell maturation, trafficking, and elimination in a mouse model of asthma. , 2003, American journal of respiratory cell and molecular biology.

[4]  K. Legge,et al.  Accelerated migration of respiratory dendritic cells to the regional lymph nodes is limited to the early phase of pulmonary infection. , 2003, Immunity.

[5]  J. Brogdon,et al.  Resident lung antigen-presenting cells have the capacity to promote Th2 T cell differentiation in situ. , 2002, The Journal of clinical investigation.

[6]  R. Coffman,et al.  A restricted subset of dendritic cells captures airborne antigens and remains able to activate specific T cells long after antigen exposure. , 2002, Immunity.

[7]  Marc Dalod,et al.  Mouse type I IFN-producing cells are immature APCs with plasmacytoid morphology , 2001, Nature Immunology.

[8]  B. Trapnell,et al.  Alveolar macrophage deficiency in osteopetrotic mice deficient in macrophage colony-stimulating factor is spontaneously corrected with age and associated with matrix metalloproteinase expression and emphysema. , 2001, Blood.

[9]  M. Jordana,et al.  Temporal-spatial analysis of the immune response in a murine model of ovalbumin-induced airways inflammation. , 2001, American journal of respiratory cell and molecular biology.

[10]  P. Holt,et al.  Ontogeny of rat pulmonary alveolar macrophage function: evidence for a selective deficiency in il-10 and nitric oxide production by newborn alveolar macrophages. , 2001, Cytokine.

[11]  R. Pauwels,et al.  Specific Migratory Dendritic Cells Rapidly Transport Antigen from the Airways to the Thoracic Lymph Nodes , 2001, The Journal of experimental medicine.

[12]  G. Rosen,et al.  Dendritic cells and macrophages in lung allografts: A role in chronic rejection? , 2000, American journal of respiratory and critical care medicine.

[13]  J. Gauldie,et al.  Transgenic expression of granulocyte-macrophage colony-stimulating factor induces the differentiation and activation of a novel dendritic cell population in the lung. , 2000, Blood.

[14]  R. Pauwels,et al.  Induction of Rapid T Cell Activation, Division, and Recirculation by Intratracheal Injection of Dendritic Cells in a TCR Transgenic Model1 , 2000, The Journal of Immunology.

[15]  Pauwels,et al.  Sensitization to inhaled antigen by intratracheal instillation of dendritic cells , 2000, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[16]  M. Jordana,et al.  Interleukin-10 gene transfer to the airway regulates allergic mucosal sensitization in mice. , 1999, American journal of respiratory cell and molecular biology.

[17]  N. Van Rooijen,et al.  Pulmonary immunity to Listeria is enhanced by elimination of alveolar macrophages. , 1999, American journal of respiratory and critical care medicine.

[18]  R. E. Wiley,et al.  GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice. , 1998, The Journal of clinical investigation.

[19]  S. Shapiro,et al.  Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. , 1997, Science.

[20]  M. Lipscomb,et al.  Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L. , 1997, American journal of respiratory cell and molecular biology.

[21]  T. Ley,et al.  Metalloelastase is required for macrophage-mediated proteolysis and matrix invasion in mice. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[22]  R. Pauwels,et al.  THE DENDRITIC CELL: ITS POTENT ROLE IN THE RESPIRATORY IMMUNE RESPONSE , 1996, Cell biology international.

[23]  R. Kradin,et al.  The antigen-presenting activities of Ia+ dendritic cells shift dynamically from lung to lymph node after an airway challenge with soluble antigen , 1995, The Journal of experimental medicine.

[24]  H. Hoogsteden,et al.  Dendritic cells and their precursors isolated from human bronchoalveolar lavage: immunocytologic and functional properties. , 1994, American journal of respiratory cell and molecular biology.

[25]  R. Beelen,et al.  Migration of dendritic cells into the draining lymph nodes of the lung after intratracheal instillation. , 1993, American journal of respiratory cell and molecular biology.

[26]  A. Pollard,et al.  A role for TGF-beta in the suppression by murine bronchoalveolar cells of lung dendritic cell initiated immune responses. , 1993, Regional immunology.

[27]  R. Beelen,et al.  Separation of alveolar macrophages and dendritic cells via autofluorescence: phenotypical and functional characterizatior , 1993, Journal of leukocyte biology.

[28]  R. Beelen,et al.  T cell priming in situ by intratracheally instilled antigen-pulsed dendritic cells. , 1993, American journal of respiratory cell and molecular biology.

[29]  P. Holt,et al.  Downregulation of the antigen presenting cell function(s) of pulmonary dendritic cells in vivo by resident alveolar macrophages , 1993, The Journal of experimental medicine.

[30]  A. Pollard,et al.  Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells , 1990, The Journal of experimental medicine.

[31]  M. Rep,et al.  Macrophages in T and B Cell Compartments and Other Tissue Macrophages Recognized by Monoclonal Antibody MOMA‐2 , 1987, Scandinavian journal of immunology.

[32]  J. Coggle,et al.  The Proliferation Kinetics of Pulmonary Alveolar Macrophages , 1984, Journal of leukocyte biology.

[33]  R. Steinman,et al.  Dendritic cells are accessory cells for the development of anti- trinitrophenyl cytotoxic T lymphocytes , 1980, The Journal of experimental medicine.

[34]  G. Galfré,et al.  Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody , 1979, European journal of immunology.