Sperm morphological defects related to environment, lifestyle and medical history of 1001 male partners of pregnant women from four European cities.

BACKGROUND Recently, differences in semen quality have been found among the partners of pregnant women from four European cities: Turku, Copenhagen, Edinburgh and Paris. METHODS During this study, slides from the four centres were subjected to a centralized assessment of sperm morphology. The percentages of sperm defects were recorded using a multiple-entry classification enabling the calculation of the multiple anomalies index (MAI), which is the mean number of anomalies per abnormal sperm. The relationships between various sperm abnormalities and self-reported data on medical history, lifestyle and occupational factors were examined. RESULTS Significant differences in the MAI and most of the sperm defects were found between the four cities, and more abnormalities were found in spring than in winter. An increase in some sperm abnormalities was related to medical treatment of the mother during pregnancy, higher birthweight and previous treatment for cryptorchidism. Significant variations of several sperm defects were related to stress, weekly working time, occupational posture and metal welding, suggesting directions for further exposure studies. CONCLUSION The present study indicated that the detailed assessment of sperm abnormalities is a useful biomarker of the effect of various external factors which may qualitatively affect human spermatogenesis.

[1]  T. Nakada,et al.  Effect of immobilization stress on testicular germ cell apoptosis in rats. , 1999, Human reproduction.

[2]  J. Szöllösi,et al.  Impaired spermiogenesis as a common finding among professional drivers. , 1979, Archives of andrology.

[3]  F. Comhaire,et al.  Deterioration of sperm quality in young healthy Belgian men. , 1996, Human reproduction.

[4]  Niels Keiding,et al.  Regional differences in semen quality in Europe , 2001, Human reproduction.

[5]  S. Oehninger,et al.  Value of sperm morphology assessed by strict criteria for prediction of the outcome of artificial (intrauterine) insemination , 2009, Andrologia.

[6]  M. Forest,et al.  Effects of chronic intermittent immobilization stress on rat testicular androgenic function. , 1981, Endocrinology.

[7]  G. Centola,et al.  Seasonal variations and age-related changes in human sperm count, motility, motion parameters, morphology, and white blood cell concentration. , 1999, Fertility and sterility.

[8]  E. Bosmans,et al.  Multicenter study on reproducibility of sperm morphology assessments. , 1998, Archives of andrology.

[9]  A Spira,et al.  Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories. , 2000, Human reproduction.

[10]  R. Frydman,et al.  Sperm factors related to failure of human in-vitro fertilization. , 1986, Journal of reproduction and fertility.

[11]  G. Zielhuis,et al.  Semen quality and frequency of smoking and alcohol consumption--an explorative study. , 1995, International journal of fertility and menopausal studies.

[12]  A. Pangkahila,et al.  Occupational Influences on Male Fertility and Sexuality , 2009, Andrologia.

[13]  Pierre Jouannet,et al.  Decline in Semen Quality among Fertile Men in Paris during the Past 20 Years , 1995 .

[14]  N. Keiding,et al.  Evidence for decreasing quality of semen during past 50 years. , 1992 .

[15]  R. Kapp,et al.  An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. A report of the U.S. Environmental Protection Agency Gene-Tox Program. , 1983, Mutation research.

[16]  E. Nieschlag,et al.  External quality control in the andrology laboratory: an experimental multicenter trial. , 1990, Fertility and sterility.

[17]  E. Nieschlag,et al.  Internal quality control of semen analysis. , 1992, Fertility and sterility.

[18]  W. P. Dixon,et al.  BMPD statistical software manual , 1988 .

[19]  B. Gladen,et al.  Does early exposure to maternal smoking affect future fertility in adult males? , 1992, Reproductive toxicology.

[20]  J. Auger,et al.  Evidence for regional differences of semen quality among fertile French men. Fédération Francaise des Centres d'Etude et de Conservation des Oeufs et du Sperme humains. , 1997, Human reproduction.

[21]  A. Lenzi,et al.  Effects of prolonged autovehicle driving on male reproduction function: a study among taxi drivers. , 1996, American journal of industrial medicine.

[22]  K. Moghissi,et al.  Effects of stress and characteristic adaptability on semen quality in healthy men. , 1988, Fertility and sterility.

[23]  P. J. Rowe,et al.  WHO Manual for the Standardized Investigation and Diagnosis of the Infertile Couple , 1993 .

[24]  W. Göhde,et al.  Mammalian spermatogenesis as a biologic dosimeter for radiation. , 1981, Acta radiologica. Oncology.

[25]  P. Matson.  External quality assessment for semen analysis and sperm antibody detection: results of a pilot scheme. , 1995, Human reproduction.

[26]  D. Katz,et al.  Effects of Psychological Stress on Human Semen Quality , 1997 .

[27]  T. Demirakca,et al.  Sperm morphology assessment using strict criteria and male fertility under in-vivo conditions of conception. , 1996, Human reproduction.

[28]  A. Lenzi,et al.  Fertility and semen quality of workers exposed to high temperatures in the ceramics industry. , 1992, Reproductive toxicology.

[29]  D. Lamb,et al.  World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm‐Cervical Mucus Interaction, 4th ed. , 2000, Journal of Andrology.

[30]  S. Oehninger,et al.  Predictive value of abnormal sperm morphology in in vitro fertilization. , 1988, Fertility and sterility.

[31]  M. Bibbo,et al.  Association of diethylstilbestrol exposure in utero with cryptorchidism, testicular hypoplasia and semen abnormalities. , 1979, The Journal of urology.

[32]  Tina Kold Jensen,et al.  Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners , 1998, The Lancet.

[33]  J. Bonde.  Semen quality in welders exposed to radiant heat. , 1992, British journal of industrial medicine.

[34]  P. Jouannet,et al.  Male factors and the likelihood of pregnancy in infertile couples. I. Study of sperm characteristics. , 1988, International journal of andrology.

[35]  N. Keiding,et al.  Do environmental estrogens contribute to the decline in male reproductive health? , 1995, Clinical chemistry.

[36]  R. Kapp,et al.  An evaluation of human sperm as indicators of chemically induced alterations of spermatogenic function. A report of the U.S. Environmental Protection Agency Gene-Tox Program. , 1983, Mutation research.

[37]  J. Olsen,et al.  Birthweight and semen characteristics. , 2000, International journal of andrology.

[38]  M. Criqui,et al.  Does diet or alcohol explain the French paradox? , 1994, The Lancet.

[39]  E. Love,et al.  Association of semen quality and occupational factors: comparison of case-control analysis and analysis of continuous variables. , 1998, Fertility and sterility.

[40]  M. Vine.  Smoking and male reproduction: a review. , 1996, International journal of andrology.

[41]  R. Mieusset,et al.  Sperm Morphology in Fertile Men and Its Age Related Variation , 2009, Andrologia.

[42]  Sharpe,et al.  Male reproductive health and environmental xenoestrogens. , 1996, Environmental health perspectives.

[43]  D. Schwartz,et al.  Study of Sperm Morphologic Characteristics in a Group of 833 Fertile Men , 2009, Andrologia.

[44]  E. Bosmans,et al.  Chronobiological fluctuations in semen parameters with a constant abstinence period. , 1996, Archives of andrology.